MITOCHONDRIAL PROTEIN SYNTHESIS IN HeLa CELLS (original) (raw)

Acta Endocrinologica, 1973

Abstract

HeLa cell mitochondrial polysomes, estimated to consist of two to seven 60S monomers, exhibit an unusual resistance to RNase and EDTA, possibly related to the strongly hydrophobic nature of their polypeptide products which makes the nascent chains particularly "sticky". Mitochondria-specific ribosomal and 4S RNA are coded for by mitochondrial DNA (mit-DNA): there is one gene for each ribosomal RNA (rRNA) species and nine genes for 4S RNA on the heavy (H) mit-DNA strand, and three 4S RNA genes on the light (L) strand. The existence of messenger RNA (mRNA) transcribed from mit-DNA is strongly suggested by the occurrence in mitochondria of poly(A) stretches, 60 to 80 residues long, most of which are covalently linked to RNA molecules coded for by mit-DNA. Mitochondrial poly(A) is not a transcription product of mit-DNA. The in vivo and in vitro products of mitochondrial protein synthesis exhibit by polyacrylamide gel electrophoresis a group of not well resolved components in t...

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