Diarrhea and Enteric Emerging Viruses in HIV-Infected Patients (original) (raw)

1999, AIDS Research and Human Retroviruses

To evaluate the prevalence of enteric viruses and their possible association with diarrhea, 244 stool samples were collected from HIV-infected and uninfected patients with or without diarrhea (subgroups I-a, Ib, II-a, and II-b, respectively). Subjects were screened by polyacrylam ide gel electrophoresis, latex agglutination , and enzyme immunoassays for rotaviruses, adenoviruses, picobirnaviruses, and astroviruses. Enteric viruses were found significantly more often in specimens from HIV patients (20%) than in specimens from uninfected HIV patients (0%) (p , 0.05). Picobirnavirus was detected in 14.63% of 82 HIV-infected patients with diarrhea, but it was detected neither in those without diarrhea (0%) (p , 0.05) nor in the groups of uninfected HIV subjects (0%) (p , 0.05). Nor could astrovirus (subgroups I-a [4.00% ] versus subgroup I-b [5.26% ], p. 0.05) or enteric adenovirus (subgroup I-a [1.22% ] versus subgroup I-b [0%], p. 0.05) be linked to the diarrhea disorder in HIV-infected patients. Rotaviruses were not detected in any of the clinical subgroups studied. Enteric viruses were detected in 15 of 93 (16.13%) of the HIV-infected patients with CD4 1 T cell count , 200/m l and 3 of 19 (15.79%) of those HIV-infected individuals with a CD4 1 T cell count 200-499/m l, showing no significant difference (p. 0.05). According to our data, unusual enteric viruses such as picobirnavirus, astrovirus, and enteric adenovirus occur in HIV-infected population in Córdoba, Argentina. However, only picobirnaviruses could be significantly associated with diarrhea in these patients.