The antioxidant and anti-inflammatory effects of Eremina desertorum snail mucin on experimentally induced intestinal inflammation and testicular damage (original) (raw)
Related papers
Egyptian Journal of Chemistry, 2021
Mucin is known by its biological and pharmacological activities. The aim of current study is to evaluate the hepatoprotective and the antioxidant activities of mucin extracted from Eremina desertorum snail as well as chemical profiling using GC-MS/MS analysis. This investigation confirmed the hepatoprotective and the antioxidant effects of mucin either alone or in combination with Silymarin on carbon tetrachloride (CCl4) liver damage. Male albino mice were injected intraperitoneally with 0.5ml /kg b.wt of 40% CCl4, twice/week for 8 weeks. The treated groups treated orally with Mucin (after 8 weeks of CCl4 intoxication, twice a week for four weeks) or Silymarin (from the first day of the experiment with a dose of 140 mg/kg, three times/week until the sacrifice day). After 12 weeks, all animals were sacrificed by cervical dislocation. Results showed that CCl4 induced a hepatotoxic effect represented in increasing liver enzymes, MDA and IL-2 while decreasing total protein, albumin, CAT, SOD and GSH. Amelioration in these parameters occurred after treatment of CCl4 intoxicated mice with either Silymarin or E. desertorum mucin where they caused increases in the activity of the antioxidant parameters while, reduced the level of MDA and liver enzymes activity. Also, the histopathological alterations that caused by CCl4 were improved after the treatment with this extract. GC-MS/MS analyses led to the identification of ten compounds were categorized as monoterpenes, sesquiterpenes, quinolines, and fatty acid esters. Conclusively, E. desertorum mucin enhanced antioxidant activity and ameliorated the CCl4-induced liver damage and it could be used as a hepatoprotective agent.
Cytoprotective and antioxidant effects of the methanol extract of Eremomastax Speciosa in rats
African Journal of Traditional, Complementary and Alternative Medicines, 2013
Background: Ethno-botanical information shows that Eremomastax speciosa is used in the traditional management of various stomach complaints including gastro-duodenal ulcers. Materials and Methods: In this study, we tested the cytoprotective potential of the whole plant methanol extract (100-200 mg/kg, p.o), against HCl/ethanol, absolute ethanol, cold/restraint stress rats, and pylorus legated rats pre-treated with indomethacin. The effects of the extract on gastric lesion inhibition, the volume of gastric juice, gastric pH, gastric acid output, mucus production and gastric peptic activity were recorded. Oxidative stress parameters were measured in blood and gastric tissue samples obtained from the animals in all the models tested. Results: The extract significantly (p<0.05), reduced the formation of cold/restraint ulcers by (31-60%, inhibition), completely inhibited (100%) the formation of lesions induced by HCl/ethanol at the highest dose, but was less effective against absolute ethanol (22-46% inhibition). The extract (200 mg/kg), significantly reduced lesion formation (P<0.01), gastric acidity (P<0.01), and volume of gastric secretions (P<0.05), in the indomethacin/pylorus ligation model, and did not affect the activity of pepsin in gastric juice. Blood concentrations of antioxidant enzymes (catalase, SOD and GSH), increased significantly and MDA concentrations decreased in all models tested. Conclusion: Cytoprotection by E. speciosa methanol extract was attributed to its ability to reduce acid secretion, and to enhance mucosal defence and in vivo antioxidant status.
International Journal of Life Science and Pharma Research
Mukta Bhasma (MB) is a traditional Ayurvedic preparation for upper respiratory and lower respiratory conditions, eye disorders, powerful cardiac tonic, immune-regulator, mood elevator and known to promote strength, intellect and semen production. Mukta bhasma is prepared using direct heat as a media of transformation. Mukta Bhasma was evaluated for its hepatoprotective and antioxidant activities against Carbon tetrachloride (CCl4) induced liver damage in wistar albino rats. Mukta Bhasma (100mg/kg, 200mg/kg and 300mg/kg) was administered to experimental rats for 10 days. Silymarin (25mg/kg) was given as the standard drug. The hepatoprotective activity was assessed using various serum biochemical parameters like (SGOT), (SGPT), Total and Direct bilirubin (TB and DB), Alkaline phosphatase (ALP), Total Triglycerides (TG) and Total Cholesterol (TC). Lipid peroxidation (LPO), Reduced Glutathione (GSH) and Catalase (CAT) were determined to explain the antioxidant activity of Mukta Bhasma. ...
Food and Nutrition Sciences, 2014
Background: Green tea has been used as a daily beverage for several years. Anti-inflammatory effect of tea has also been depicted in different papers. Therefore we had set forward this study to examine the potential antiinflammatory activity of green tea in small intestine mucositis experimental models. Aims: Evaluation of antiinflammatory effects of green tea on mice. Materials and Methods: Green tea decoction (20%) was prepared by soaking 20 g of green tea in 100 ml boiled water separately, soaked for 2 minutes and thereafter filtered. Inflammatory activity was induced using methotrexate (2.75 g/kg/24 h sc), and a protecting effect of mucositis condition was investigated by vitamin E and Camellia sinensis decoction. Study Design: An experimental study was approved by an Animal Ethical Commitee. Results: Green tea decoction (20%) has shown significant anti-inflammatory effects (65% and 70%) on methotrexate-induced acute mucositis model. In villous atrophy Green tea decoction (10% and 20%) has shown no protecting action at different intestinal segments. But at intestinal crypt hyperplasia, green tea decoction has shown 65.74%, as compared to mucositis group. An increase of apoptotic bodies were acchieved at MTX group, CS reduced this occurrence. Conclusion: Taken together, our data indicate that green tea (20%) has a potential anti-inflammatory compared vitamin E antioxidant action and corroborates with the current trend of tea being promoted as "health drink". However more pharmacological and biochemical assays is necessary to elucidate mechanisms.
Journal of research in pharmacy, 2020
Despite widespread traditional usage of Eryngium species in Anatolia (Turkey), only a limited number of scientific studies exists on E. kotschyi, an endemic species. Previously, extracts from E. campestre and E. kotschyi were reported to have significant anti-inflammatory effects in vivo. This study aimed to investigate the in vitro antiinflammatory, antioxidant, urease inhibitory activities of ethanol extracts of E. kotschyi and E. campestre var. virens roots as well as distilled water and ethanol extracts of E. kotschyi aerial parts. The NO and cytokine inhibitory effects were evaluated by Griess and ELISA assays. The antioxidant activities were tested on DPPH • , ABTS •+ and CUPRAC assays. The EtOH extract of E. kotschyi roots (EKr EtOH) and aerial parts (EKae EtOH) inhibited 50.08% and 41.52% of NO production at 100 µg/ml, respectively. The EtOH extract of the roots of E. campestre var. virens (ECr EtOH) and EKr EtOH provided 36.22% and 65.23% IL-6 inhibition and 44.24% and 56.84% IL-1α inhibition at 100 µg/ml. EKae EtOH exerted highest antioxidant activity on ABTS •+ (2.4±0.0005 µM trolox/mg extract) and CUPRAC (0.97±0.07 mM trolox/mg extract). This extract was also found the richest among all in terms of phenolics content (6.1±0.001 mg/GAE/g extract). EKr EtOH and ECr EtOH exhibited strongest DPPH • (IC50 = 1.132±0.057 mg/ml) radical scavenging and ferric reducing/antioxidant power (0.36±0.005 mM Fe2+/mg extract) activity respectively. The extracts exerted low urease inhibitory activity. Consequently, the results of this study might contribute to the elucidation of the mechanism of in vivo anti-inflammatory activity of the extracts.
Journal of Medicinal Plants Studies, 2017
The study evaluated antioxidant potentials of Laportea aestuans and chemopreventive potentials on urinary inflammatory markers using albino rats. The rats were fed with 10%, 30% and 50% Laportea aestuans. Results indicates a reduction in nitric oxide levels compared to control (P<0.05) while concentration of polyphenols in urine were significant with increased percentage of Laportea aestuans supplemented diet. This study also showed reductive potencies of Laportea aestuans in the concentration of protein in urine. Results also showed an increase in the activities of CyP1A1 microsomal enzymes in the test groups compared to control (P<0.05). The activities of Glutathion Stransferase, Quinone oxidoreductase and Thioredoxin reductase increased significantly in all the test samples with increasing percentage of Laportea aestuans compared to control. This is indicative of the potential health benefit of Laportea aestuans in chemoprevention. On this basis, Laportea aestuans have show...
Cyrtocarpa procera is a plant used in traditional Mexican medicine to treat different gastrointestinal problems. Here, we investigated the effects of a C. procera methanolic extract in DSS-induced colitis mice. Ulcerative colitis (UC) was induced by administering 4% DSS in drinking water to female BALB/c mice. Compared to untreated mice with UC, the treatment group receiving the C. procera extract presented less severe UC symptoms of diarrhea, bleeding, and weight loss. Additionally, colon shortening was significantly reduced, and at the microscopic level, only minor damage was observed. Levels of proinflammatory cytokines such as TNF-α, IL-1β, and IFNγ in serum as well as the MPO activity in the colon were significantly reduced in the C. procera methanolic extract-treated group. Moreover, the extract of C. procera reduced oxidative stress during UC, preventing the deterioration of the activity of antioxidant enzymes such as SOD, CAT, and GPx. Additionally, the extract decreased lipid peroxidation damage and its final products, such as malondialdehyde (MDA). In agreement with this, in vitro assays with the C. procera extract displayed good antioxidant capacity, probably due to the presence of polyphenolic compounds, in particular the flavonoids that were identified, such as chrysin, naringenin, kaempferol, and catechin, which have been reported to have antiinflammatory and antioxidant activities. Therefore, the improvement of UC by the C. procera methanolic extract may be related to the action mechanisms of these compounds.