Morphology and molecular phylogeny of Heterobothrium lamothei (Monogenea: Diclidophoridae), a gill parasite infecting the tiger puffer fish Lagocephalus sceleratus (Tetraodontidae) (original) (raw)
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Journal of the Egyptian Society of Parasitology, 2013
Heterobothrium lineatus (Monogenea: Diclidophoridae) is described from the gills of Tetraodon lineatus collected from the River Nile at Helwan governorate, Egypt as a new locality record. The morphology and morphometric characterization of the recovered worms were described by means of light and scanning electron microscopy. Twenty two out 35 with a percentage of 62.9% of the examined fish were infected with Heterobothrium sp. (the intensity of infection was about ten worms per fish in general). Most of the infected fish had very pale gills and showed symptoms of anemia. Morphologically, the adult worms were elongated with anterior pointed and posterior broad ends, it measured 1.15-1.76 (1.52±0.02) mm in length x 0.28-0.39 (0.29±0.02) mm in width. Two buccal organs situated anteriorly around mouth opening were shown by light and scanning electron microscopy. Haptor subdivided into four pairs of clamps without isthmus separating it from body. The recovered worm differed from the previously species in the same genus by small dimensions of the measurements and presence of a copulatory organ armed with 7-11 genital hooks. Also, it is distinguished from H. tetrodonis and H. okamotoi by absence of a distinct isthmus, and resembled H. lamothei from gills of Sphoeroides testodineus in Mexico and H. lineatus from T. lineatus in Egypt in general appearance and presence of rectangular haptor with the fourth pair of clamps smaller than the previous ones.
Journal of Veterinary Research
Introduction Monogenea is a class of ectoparasitic flatworms on the skin, gills, or fins of fish. Microcotylidae is a family of polyopisthocotylean monogeneans parasitising only marine fishes. This work describes and taxonomically determines a microcotylid polyopisthocotylean monogenean in an important fish in Saudi aquaculture. Material and Methods Thirty gilt-head sea bream captured alive from the Red Sea of Saudi Arabia were examined for monogenean infection. Worms were described morphologically and morphometrically by light microscopy and multiple sequence alignments and phylogenetic trees were also constructed after maximum likelihood analysis of the 28S rRNA sequences. Results Seventeen fish were infected by a monogenean parasite in the gill lamellae. It showed a bilobed anterior extremity, two rows of numerous unequal clamps of microcotylid type, and paired muscular vaginae crowned by differently sized spines. The vaginal number and its relative armature suggested the species...
Veterinary Research Forum, 2021
During a recent survey on digenean parasites infecting marine fish captured from water located along the Red Sea off Gizan Coasts, Saudi Arabia, thirty-two (29.09%) out of 110 fish were naturally infected with three different species of digenean parasites. The taxonomical status of the recovered worms was confirmed by light microscopy and molecular analysis. Transversotrema licinum infecting the Haffara seabream Rhabdosargus haffara possessed a transversely elongated body with eyespots and the anterior body margin with delineated velum along with its entire breadth. Oral sucker was absent, and eyespots were paired anterior to or at the level of ventral sucker. Phyllodistomum hoggettae from the urinary bladder of the Arabian Nagil Plectropomus areolatus was differentiated by its elongated body, narrow anterior end widened as going posteriorly with prominent marginal undulations in the hindbody. Gyliauchen volubilis infecting the marbled spinefoot Siganus rivulatus, was with fleshy bo...
ZooKeys, 2018
During the examination of 913 fish specimens belonging to four families in the Campeche Bank (Gulf of Mexico), 23 gill ectoparasitic monogenean species were found, which belong to three families: Dactylogyridae, Microcotylidae and Diclidophoridae. The speciesEuryhaliotremaamydrum,E.carbuncularium,E.dunlapae,E.fajeravilae,E.fastigatum,E.longibaculum,E.paracanthi,E.tubocirrus,Haliotrematoidescornigerum,H.gracilihamus,H.heteracantha,H.longihamus,H.magnigastrohamus,H.striatohamus,Hamatopedunculariabagre,Neotetraonchusbravohollisae, andN.felis(all Dactylogyridae) were found on the hostsLutjanussynagris,L.griseus,Ariopsisfelis,Bagremarinus,Archosargusrhomboidalis, andHaemulonplumieri. Additionally,Microcotylearchosargi,Microcotylesp., andMicrocotyloidesincisa(all Microcotylidae) were found onL.griseusandA.rhomboidalis; finally,Choricotylesp. 1,Choricotylesp. 2, andChoricotylesp. 3 (all Diclidophoridae) were found onH.plumieri. The prevalence, abundance, mean intensity of infection, and su...
Parasites & Vectors, 2013
Background: The Sultanate of Oman's aquaculture industry is expanding with an ongoing assessment of potential new fish species for culture. The king soldier bream, Argyrops spinifer (Forsskål) (Sparidae), is one such species that is under consideration. During a routine health assessment of specimens caught in the Sea of Oman throughout the period November 2009 to March 2011, a number of gill polyopisthocotylean monogeneans were recovered. Methods: A subsequent study of the monogeneans using a range of morphology-based approaches indicated that these were Bivagina heterospina Mamaev et Parukhin, 1974. In the absence of pre-existing molecular data, an expanded description of this species is provided, including a differential diagnosis with other species and genera belonging to the subfamily Microcotylinae Monticelli, 1892 with the subsequent movement of this species to a new genus to accommodate it. Results: The polyopisthocotyleans collected from the gills of A. spinifer appear to be unique within the family Microcotylidae Taschenberg, 1879 in that, morphologically, they possess a pair of large, muscular vaginae each armed with a full crown of 16-18 robust spines and a unique dorsal region of folded tegument, which permits their discrimination from species of Bivagina Yamaguti, 1963. Sequencing of the SSU rDNA (complete 1968 bp) and LSU rDNA (partial 949 bp) places the specimens collected during this study within the subfamily Microcotylinae, but the LSU rDNA sequence differs from Bivagina and also from other microcotylid genera. Morphological features of B. heterospina sensu Mamaev et Parukhin, 1974 and the specimens collected from the current study are consistent with one another and represent a single species. The vaginal armature of these worms is unique and differs from all other genera within the Microcotylinae, including Bivagina, and its movement to Omanicotyle n. gen. to accommodate this species is proposed. Conclusions: A new genus, Omanicotyle n. gen., is erected to accommodate Omanicotyle [Bivagina] heterospina n. comb. which represents the first monogenean to be described from Omani marine waters. Given the pathogenic potential of microcotylids on captive held fish stocks, a full assessment of Omanicotyle heterospina n. gen. et n. comb. is now required before large-scale production commences.
Kafkas Universitesi Veteriner Fakultesi Dergisi, 2014
Parasites imported with dwarf gourami fish may present risks, both to native fish populations and to the aquaculture industry. Heteronchocleidus buschkieli Bychowsky, 1957 is an important Anabantidae fishes parasite. A large number of imported anabantid fish originate from Southeast Asian countries. The objective of this study was to investigate the occurrence of infection with monogenean parasite in dwarf gourami fish imported from Southeast Asia into Iran. In this study, a total of 400 dwarf gouramies were randomly selected from aquaria containing this fish imported from Southeast Asia into Iran. After euthanizing, the gills were removed and arches and skin were examined microscopically for monogenean parasites. Heteronchocleidus buschkieli (Monogenea: Ancyrocephalidae) was the only monogenean parasite identified morphologically in 120 of 400 (30%) imported fish. The infected fish were lethargic and had hyperemic gills with small skin lesions. Prevalence, abundance and mean intensity of infection for four years were 0.3, 30 and 30±1.96, respectively. To the author's knowledge, this is the first report of dwarf gourami infection with H. buschkieli in Iran. In order to prevent the introduction of this parasitic pathogen from imported fishes, we recommend imported fishes should be examined and treated before domestic distribution and quarantine practices should be implemented.
Egyptian Journal of Veterinary Sciences
Egyptian Journal of Veterinary Sciences https://ejvs.journals.ekb.eg/ T HIS STUDY aimed for the description of two monogenean parasites isolated from two marine fish. Worms were classified morphologically as well as by molecular analysis. Monogeneans are ectoparasitic flatworms that live on the gills, fins, and skin of fish. The Diplectanidae are a family of monopisthocotylean monogeneans. They are all parasitic on the gills of marine fish. In the present study, a total of 25 of the white-spotted spine foot, Siganus canaliculatius (Siganidae), and 35 of the cinnabar goatfish, Parupeneus heptacanthus (Mullidae), were captured from the Red Sea near Egypt's Hurghada coast. Ten spinefoot fish and 16 goatfish were infected. Morphological characterization for the isolated parasites according to the light micrographs of the hard parts as well as the copulatory organ revealed that the infected fish parasitized by Tetrancistrum sigani (Dactylogyridae) and Haliotrema banana (Ancyrocephalidae). Also, ribosomal DNA of the isolated parasites was processed and sequenced where the recovered sequences were compared with previously deposited species in the gene bank, it was observed from the constructed phylogenetic tree that the species of Tetrancistrum sequences (present study) isolates were closely related to T. sigani (accession no. MN179335.1) deposited in GenBank, while the sequences of Haliotrema isolates of the present study were closely related to the same species by 96%.
Parasitology International, 2015
The Diclybothriidae (Monogenoidea: Oligonchoinea) includes specific parasites of fishes assigned to the ancient order Acipenseriformes. Phylogeny of the Diclybothriidae is still unclear despite several systematic studies based on morphological characters. Together with the closely related Hexabothriidae represented by parasites of sharks and ray-fishes, the position of Diclybothriidae in different taxonomical systems has been matter of discussion. Here, we present the first molecular data on Diclybothriidae. The SSU rRNA gene was used to investigate the phylogenetic position of Paradiclybothrium pacificum and Diclybothrium armatum among the other Oligonchoinea. Complete nucleotide sequences of P. pacificum and D. armatum demonstrated high identity (98.53%) with no intraspecific sequence variability. Specimens of D. armatum were obtained from different hosts (Acipenser schrenckii and Huso dauricus); however, variation by host was not detected. The sequence divergence and phylogenetic trees data show that Diclybothriidae and Hexabothriidae are more closely related to each other than with other representatives of Oligonchoinea.
Emperor fishes, Lethrinus nebulosus are commercially important fishes in Saudi market. Despite of importance of these fishes, there is not a comprehensive report about parasitic fauna of these fishes. The aim of this research article is to determine the parasitic helminthes infecting these fishes in Saudi Arabia. For this purpose, 28 emperor fishes have been bought and examined by routine parasitological methods to find that 28.6% emperor fishes revealed intestinal trematode infections. Two trematodes were recognized in the fishes, Neolepidapedon (Neolepidapedon) macrum Overstreet, 1969 was found in 7 fishes (25%) and Opisthomonorcheides decapteri Parukhin, 1966 was found infecting one fish only. Scanning electron microscope had shown absence of spines and revealed scale-like structure. Saudi Arabia is considered a new locality of those two trematodes and Emperor fishes as new hosts.