Plant mediated green synthesis, characterization and biological study of silver nanoparticles from Ocimum gratissimum aqueous leaf extract (original) (raw)
Related papers
2019
Ocimum gratissimum leaves were used in the synthesis of silver nanoparticles (AgNps) that were non-toxic and also possessed antimicrobial properties against clinically isolated pathogenic strains. Some bioactive agents in the aqueous extract of Ocimum gratissimum leaves (AEOgL) were identified. AgNp synthesis was carried out by incubating the AEOgL and 1 mM AgNO3. The brownish colour obtained upon reduction of silver by the AEOgL was observed. The phytochemicals present are tannins, glycosides, sterols, phenols, alkaloid and terpenoids. There was the presence of a surface plasmon resonance from the UV-visible scan. The synthesized silver nanoparticles were characterized by UV-visible spectrophotometry, Fourier Transform Infrared spectroscopy (FTIR), Scanning Electron Microscopy/Energy Dispersive Analysis (SEM/EDX) and X-ray diffraction analysis. The SEM/EDX analysis indicated that the morphology of the nanoparticles is of a uniform structure and the size of the AgNp was 20 nm. The A...
Green synthesis of silver nanoparticles using Ocimum leaf extract and their characterization
Digest J Nanomat …, 2011
The metallic nanoparticles have great attention of Chemists, Physists, Biologists and Engineers who wish to use them for development of new generation nanodevices. In this present study silver nanoparticles were synthesized from aqueous silver nitrate (1mM) through a simple and ecofriendly route using leaf broth of Ocimum sanctum as reductant and stabilizer. The aqueous silver ions when exposed to leaf broth were reduced and resulted in the green synthesis of silver nanoparticles ranges from 3-20 nm. The bioreduced silver nanoparticles were characterized by UV-Vis spectrophotometer, X-ray diffraction (XRD), Transmission electron microscopy (TEM) and Fourier transform infra-red (FTIR) spectroscopy. The observed peaks in XRD pattern corresponding to , and planes. The FTIR measurement was carried out to identify the possible biomolecules responsible for efficient stabilization of silver nanoparticles (
Shanlax International Journal of Arts, Science and Humanities, 2020
Nanoparticles (NPs) are being widely used in different fields; therefore, there is growing interest in the development of a biological and environmental safety method for their production. Now a day’s chemical and physical methods are being used for the development of nanoparticle that is costly, time-consuming, and harmful for the environment. Plant-mediated synthesis of nanoparticles is a “Green chemistry” approach that connects different types of plants with nanotechnology. It has gained much more attention as a reliable, sustainable, and eco-friendly method for synthesizing a wide range of materials/nanomaterials. Plants are called nature’s “chemical factories” therefore, plants and plant extracts are the best options to produce different types of nanoparticles. In this present study, silver nanoparticles were synthesized by using leaf extract of Ocimum sanctum and aqueous silver nitrate solution through a simple and eco-friendly method. Then the developed silver nanoparticles w...
IAEME PUBLICATION, 2019
Plant extract from Ocimum sanctum was used for the green synthesis of silver nanoparticles (AgNPs) from silver nitrate solution. Antimicrobial activities of the silver nanoparticles were tested by Agar well dif usion method against Salmonella Typhi and Proteus Vulgari. The greatest antimicrobial activity for silver nanoparticles synthesized by Ocimum sanctum extract was found against Salmonella Typhi (21 mm) and Proteus vulgari (24 mm) respectively. The green synthesis of silver nanoparticles has efficient antimicrobial activity against pathogenic bacteria. Hence, silver nanoparticles are playing a significant role in nano-medicine.
Scientific reports, 2017
The rewards of using plants and plant metabolites over other biological methods for nanoparticle synthesis have fascinated researchers to investigate mechanisms of metal ions uptake and bio-reduction by plants. Here, green chemistry were employed for the synthesis of silver nanoparticles (AgNPs) using leaf extracts of Ocimum Sanctum (Tulsi) and its derivative quercetin (flavonoid present in Tulsi) separately as precursors to investigate the role of biomolecules present in Tulsi in the formation of AgNPs from cationic silver under different physicochemical conditions such as pH, temperature, reaction time and reactants concentration. The size, shape, morphology, and stability of resultant AgNPs were investigated by optical spectroscopy (absorption, photoluminescence (PL), PL-lifetime and Fourier transform infrared), X-ray diffraction (XRD) analysis, and transmission electron microscopy (TEM). The enhanced antibacterial activity of AgNPs against E-Coli gram-negative bacterial strains ...
International Journal of Applied Sciences and Biotechnology, 2018
The green route of metal nanoparticles synthesis has received significant attention in recent years due to it's cost-effective, non-toxic and eco-friendly nature in comparison to other physical and chemical methods. This study reports on the synthesis of silver nanoparticles (Ag-NPs) from bio-reduction of 1mM aqueous silver nitrate (AgNO3) by extracts prepared from three different plants namely, Brassica oleracea L. var. italica Plenck (Broccoli), Capsicum annuum L. (Chili) and Parthenium hysterophorus L. (Carrot grass). The synthesized Ag-NPs were characterized using UV-visible spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM). Ag-NPs synthesized showed the surface plasmon resonance with the appearance of absorption peaks around the range of 410-430 nm. The possible biomolecules involved in the reduction and the stabilization of synthesized Ag-NPs were found to be alcoholic, phenolic, amine and carbonyl groups. SEM study revealed that Ag-NPs were spherical in shape with varied size about 10-40 nm. Besides, the analysis of antioxidant and antibacterial activities of Ag-NPs was carried out. The Ag-NPs synthesized using B. oleracea extract showed the higher antioxidant activity than Ag-NPs synthesized from both C. annuum & P. hysterophorus extracts. Ag-NPs exhibited good antibacterial activity against both Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria. The higher antibacterial activity was shown by Ag-NPs synthesized from P. hysterophorus extract in comparison to Ag-NPs synthesized from both C. annuum & B. oleracea extracts. Hence, it can be concluded that Ag-NPs synthesized following the green route could be the source for potential antioxidant and antibacterial agents.
Journal of Nanoparticle …, 2011
Development of green nanotechnology is generating interest of researchers toward ecofriendly biosynthesis of nanoparticles. In this study, biosynthesis of stable silver nanoparticles was done using Tulsi (Ocimum sanctum) leaf extract. These biosynthesized nanoparticles were characterized with the help of UV-vis spectrophotometer, Atomic Absorption Spectroscopy (AAS), Dynamic light scattering (DLS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and Transmission electron microscopy (TEM). Stability of bioreduced silver nanoparticles was analyzed using UV-vis absorption spectra, and their antimicrobial activity was screened against both gram-negative and grampositive microorganisms. It was observed that O. sanctum leaf extract can reduce silver ions into silver nanoparticles within 8 min of reaction time. Thus, this method can be used for rapid and ecofriendly biosynthesis of stable silver nanoparticles of size range 4-30 nm possessing antimicrobial activity suggesting their possible application in medical industry.
2020
The silver nanoparticles have found prominence in different fields such as medicine, catalysis, nanoelectronics, textile field, pollution and water treatment due to their unique attributes. Applications of silver nanoparticles are increasing rapidly in the medical purpose including drug delivery, treatment, diagnosis, medical device coating. Various chemical and physical methods are use to synthesize the silver nanoparticles (AgNP) conventionally. But these synthesis processes are expensive and also involves side effects. To solve these savior problems by modification in synthesis process for safer and more efficiency, Green Nanotechnology comes to play a very crucial role for synthesis of
2019
To resolve the efficacies of antibacterial, antifungal, and antioxidant activities of aqueous leaf extract of Ocimum basilicum mediated biosynthesis of silver nanoparticles (Ag-NPs). Aqueous and synthesized Ag-NPs were tested by disc diffusion method against the bacterial and fungal pathogens. Antioxidant activity of synthesized Ag-NPs and aqueous plant extract were determined. The scavenging radicals were estimated by DPPH method. The synthesized Ag-NPs were characterized by XRD, FT-IR, FESEM and EDX. XRD peaks at 2θ values of 27.6°, 32.2°, 38.16°, and 46.04° representing the (110), (111), (121), and (200). FT-IR absorption peak located at 2910 cm-1 are due to C–H stretch of alkenes and a peak at 1669 cm-1 due to -C=C- band of alkenes and a peak at 1614 cm-1 is assigned to N-H bend of 1° amines and a peak at 1092 cm-1 due to C–N stretch of aliphatic amines and a peak at 875 cm-1 are due to C–H stretch of aromatics. TEM study reveals that most of the nanocrystals formed were spheric...
Rapid Biological Synthesis of Silver Nanoparticles from Ocimum sanctum and Their Characterization
Journal of Nanoscience, 2017
With development of nanotechnology, the biological synthesis process deals with the synthesis, characterization, and manipulation of materials and further development at nanoscale which is the most cost-effective and eco-friendly and rapid synthesis process as compared to physical and chemical process. In this research silver nanoparticles (AgNPs) were synthesized from silver nitrate (AgNO 3) aqueous solution through eco-friendly plant leaf broth of Ocimum sanctum as reactant as well as capping agent and stabilizer. The formation of AgNPs was monitored by ultraviolet-visible spectrometer (UV-vis) and Fourier transform infrared (FTIR) spectroscopy. X-ray diffraction (XRD) and scanning electronic microscopy (SEM) have been used to characterize the morphology of prepared AgNPs. The peaks in XRD pattern are in good agreement with that of face-centered-cubic (FCC) form of metallic silver. Thermal gravimetric analysis/differential thermal analysis (TGA/DTA) results confirmed the weight loss and the exothermic reaction due to desorption of chemisorbed water. The average grain size of silver nanoparticles is found to be 29 nm. The FTIR results indicated that the leaf broths containing the carboxyl, hydroxyl, and amine groups are mainly involved in fabrication of silver AgNPs and proteins, which have amine groups responsible for stabilizing AgNPs in the solution.