Evidence for a stereospecific [3 H]etorphine binding in human placenta (original) (raw)
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Biochemical Pharmacology, 1980
Biochemical Pharmacology, Vol. 29, pp. 2657-2661. Pergamon Press Ltd. 1980. Printed in Great Britain. 0006-2952/80/1001-2657 $02.00/0 SPECIFIC BINDING FOR OPIATE-LIKE DRUGS IN THE PLACENTA A. VALETTE,* JM REME,* G. PONTONNIER* and J. CROS+ Inserm ...
Cell Journal (Yakhteh), 2012
Objective: In previous studies it has been emphasized that the site of morphine action may be either in the embryo or the placenta. In the present study, we attempt to identify the site of morphine action on the fetal section of Wistar rat placenta by using C14-morphine. Materials and Methods: In this study (experimental), female Wistar rats (weights: 170-200 g) were mated with male rats and their coupling times recorded. Experimental groups received daily doses of 0.05 mg/ml of C14-morphine in their drinking water. On the 9th and14th embryonic days, the pregnant rats were anesthetized and the placenta and uterus surgically removed. Placentas were fixed in 10% formalin for two weeks, then processed, sectioned in 5 µm and 25 µm thicknesses, and fixed on glass slides for further evaluation. The 25 µm sections were delivered to black and white film for three days. Films were processed and evaluated with a digital inverse microscope for possible radiological impression. The 5 µm section...
Iranian journal of reproductive medicine, 2011
Previous studies have shown that morphine consumption during pregnancy may delay embryo development or cause abnormal nervous system function. The present study focused on the effect of maternal morphine consumption on development of placenta and blood corticosteron concentration in addictive pregnant mothers. 24 female rats, 170-200g weight, were used. The experimental groups after pregnancy received an oral dose of 0.05 mg/ml of morphine by tap water while the control group received only tap water. On 10(th) and 14(th) day of pregnancy, rats were anesthetized and placenta removed surgically, 1ml blood was collected from each pregnant mother from retro-orbital sinus, the concentration of blood corticosteron was determined by corticosteron Elisa kit after centrifugation. The fixed tissue was processed, sectioned and stained with hematoxylin and eosin. Placenta was studied microscopically according to the thickness of layers, area of blood cisterns, and the number of cells. Comparing...
Binding of the non-selective opioid [3H]etorphine in the human fetal central nervous system
Developmental Brain Research, 1988
The existence of specific, high-affinity opioid binding sites was demonstrated in 21-week-old human fetuses with the use of tritiated etorphine, a non-selective opioid ligand. Binding capacities measured in the brain, the cerebellum and the spinal cord give values of 2.83, 3.71 and 5.42 pmol/g of tissue. Kd values do not vary from one region to the other and are respectively 0.29, 0.35 and 0.33 nM. These results are compared with the characteristics of opioid binding sites found in adults.
Defects in Wistar Rat’s Embryo and Placenta Development: A [C]14-Morphine Study
Annual Research & Review in Biology, 2014
Objective: In previous studies it has been emphasized that the site of morphine action may be either in the embryo or the placenta. In the present study, we attempt to identify the site of morphine action on the fetal section of Wistar rat placenta by using C 14-morphine. Materials and Methods: In this study (experimental), female Wistar rats (weights: 170-200 g) were mated with male rats and their coupling times recorded. Experimental groups received daily doses of 0.05 mg/ml of C 14-morphine in their drinking water. On the 9 th and14 th embryonic days, the pregnant rats were anesthetized and the placenta and uterus surgically removed. Placentas were fixed in 10% formalin for two weeks, then processed, sectioned in 5 µm and 25 µm thicknesses, and fixed on glass slides for further evaluation. The 25 µm sections were delivered to black and white film for three days. Films were processed and evaluated with a digital inverse microscope for possible radiological impression. The 5 µm sections were processed for hematoxylin and eosin (H&E) staining, and evaluated by light microscope and MOTIC software. Results: Our results indicated that the site of action of C 14-morphine was possibly located on the blood plexus of the fetal portion of the placenta. In addition, oral morphine consumption was shown to inhibit fetal and maternal placental development in the experimental groups. Conclusion: We conclude that morphine's effectiveness on the reduction of embryo growth and development may be via its effects on the blood plexus of the fetal section of the placenta.
Clinical and Developmental Immunology, 2013
Previous studies have shown that morphine abuse during pregnancy cancause a delay in the development of the placenta and embryo and also bring about birth defects. The present study investigates the effect of the duration of maternal morphine consumption during pregnancy, as well as the impacts of morphine abuse on the development of placental layers during the three different periods of pregnancy in Wistar rats.Materials and Methodology. Female Wistar rats have been used in the present study. Experimental groups received morphine (0.05 mg/mL of drinking water) after one night of coupling with male rats for mating. On 9th, 10th, and 14th days of pregnancy, pregnant animals were killed, and placentas were removed and fixed. The cells of the placentas layers were calculated by light microscope and MOTIC and SPSS software.Results. The maternal surface thickness of the placenta was significantly increased, whereasthe fetal surface thickness of placenta was significantly decreased with m...
2015
Copyright © 2013 Leila Dehghani et al.This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Previous studies have shown that morphine abuse during pregnancy cancause a delay in the development of the placenta and embryo and also bring about birth defects. The present study investigates the effect of the duration of maternal morphine consumption during pregnancy, as well as the impacts of morphine abuse on the development of placental layers during the three different periods of pregnancy in Wistar rats.Materials and Methodology. Female Wistar rats have been used in the present study. Experimental groups received morphine (0.05mg/mL of drinking water) after one night of coupling with male rats for mating. On 9th, 10th, and 14th days of pregnancy, pregnant animals were killed, and placentas were removed and fixed.The cells of ...