BMP Signaling Is Crucial for Regulation Vascular Progenitor Development in Human Embryonic Stem Cells (original) (raw)

Blood, 2009

Abstract

3037 Poster Board II-1013 The generation of vascular cells from pluripotent stem cells, including human embryonic stem cells (hESCs) and induced pluripotent stem (iPS) cells, may facilitate tissue transplantation, reperfusion of ischemic tissues, and treatment of pathologies in which endothelial cell dysfunction exists. The signals that direct pluripotent stem cell differentiation into lineage-specific cells remain largely unknown. To identify vascular progenitor cells during hESC differentiation, we characterized various subpopulations that may differentiate into endothelial cells (ECs) and smooth muscle cells (SMCs). In our newly established serum-free medium, hESCs sequentially differentiated into CD34+CD31-, CD34+CD31+, and then CD34-CD31+ cells. Real-time PCR analysis indicated that EC- and SMC-specific genes, including VEGFR2, Tie2, VE-Cad, vWF, SMA, SM22-alpha, calponin, and caldesmon, were expressed significantly high in CD34+CD31+ cells. Furthermore, the CD34+CD31+ cells th...

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