Blend of tetracycline and AgNPs synthesized from Alternaria have potentiality as antibacterial drug * (original) (raw)

Fungus- (Alternaria sp.) Mediated Silver Nanoparticles Synthesis, Characterization, and Screening of Antifungal Activity against Some Phytopathogens

Journal of Nanotechnology

The scientific consensus is now on developing a biocontrol agent that can cause cellular metabolic reprogramming against agricultural pathogens. Biosynthesis of silver nanoparticles was performed by using phytopathogenic fungi (Alternaria sp.) isolated from banana cultivated soil. Alternaria sp. can grow very fast and produce high enough bioactive compounds. This study aims to biosynthesize silver nanoparticles (AgNPs) using fungal Alternaria sp.’s metabolites as a safe antifungal agent against plant pathogenic fungi (Fusarium spp. and Alternaria sp.). To visualize the formation of AgNPs, analytical instruments were used, such as ultraviolet-visible (UV-Vis) spectroscopy, Fourier-transform infrared (FTIR) spectroscopy, scanning transmission electron microscopy (STEM), energy dispersive X-ray (EDX), and elemental mapping. The UV-visible spectra showed a peak at 435 nm. Analysis of scanning transmission electron microscopy (STEM) micrographs evidenced that the size of synthesized silv...

Potentially Bioactive Fungus Mediated Silver Nanoparticles

Nanomaterials

Fungal metabolites, proteins, and enzymes have been rich sources of therapeutics so far. Therefore, in this study, the hypha extract of a newly identified noble fungus (Alternaria sp. with NCBI Accession number: MT982648) was used to synthesize silver nanoparticles (F-AgNPs) to utilize against bacteria, fungi, and lung cancer. F-AgNPs were characterized by using physical techniques, including UV–visible spectroscopy, zeta potential, DLS, XRD, TEM, and HR-TEM. The particles were found to be polydispersed and quasi-spherical in shape under TEM. They had an average size of ~15 nm. The well dispersed particles were found to have consistent crystallinity with cubic phase geometry under XRD and HR-TEM. The presence of different functional groups on the surfaces of biosynthesized F-AgNPs was confirmed by FTIR. The particle distribution index was found to be 0.447 with a hydrodynamic diameter of ~47 d.nm, and the high value of zeta potential (−20.3 mV) revealed the stability of the nanoemul...

Fungal mediated synthesis of silver nanoparticles and evaluation of antibacterial activity

Microscopy Research and Technique, 2019

Nanoparticles as biomedicine has made a crucial role in health biotechnology. Different transition metals in various forms playing role in nanotechnological advances and biological applications. Silver as one of the nontoxic, safe inorganic antibacterial agents and can serve as replacement of antibiotics. Present research is based on biogenic synthesis of silver nanoparticles (Ag-NPs) as potential antibiotics from fungal metabolites of Penicillium oxalicum. We used different analytical techniques X-ray diffraction (XRD) and scanning electron microscopy (SEM) for characterization of biosynthesized silver nanoparticles. Furthermore, the antibacterial activity of biosynthesized silver nanoparticles was checked against Staphylococcus aureus, S. dysenteriae, and Salmonella typhi by using well diffusion method and UV visible spectrophotometer. Maximum zone of inhibition recorded against S. aureus, Shigella dysenteriae was 17.5 ± 0.5 mm (mm) for both species and 18.3 ± 0.60 mm for Salmonella typhi. The biosynthesized silver nanoparticles of P. oxalicum showed excellent antibacterial activity. It was concluded from our results that biosynthesized silver nanoparticles have significant potential and might be useful for a wide range of biological applications such as bactericidal agent against resistant bacteria, preventing infections, healing wounds, and antiinflammation.

Fungus-mediated biosynthesis of silver nanoparticles and its antibacterial activity

2014

Silver is an active area of academic and more importantly in nanotechnology for application research. This source of production of nanoparticles can be exploited further commercially. In the present study, we have reported biological synthesis of silver nanoparticles by using cell free filtrate of Helminthosporium tetramera. Synthesis of Silver nanoparticles was carried out using fungal cell free filtrate and 1mMaqueous solution of silver nitrate (Ag No3). The synthesis of silver nanoparticles was investigated by UV-Vis spectroscopy. Absorbance was recorded from 300-600 nm and strong absorbance peak of AgNPs was observed at 400 nm. The obtained Silver nanoparticles were characterized by Transmission electron microscopy (TEM). The synthesized silver nanoparticles were polydisperse spherical in shape and size in the range between 17-33 nm. The antibacterial activity of AgNPs have been evaluated against the Bacillus subtilis and Staphylococcus sp. AgNPs were found to have significant antibacterial activity, thus product could be used as an effective antibacterial agent.

Biosynthesis and characterization of silver nanoparticles using Agrocybe cylindracea, and their antibacterial activity

Scientific Journal for Damietta Faculty of Science

Mushrooms are of great interest in nanotechnology. Antibiotics resistance is a serious condition that threatens the treatment of many diseases caused by bacteria. So, it becomes persistent to find new antibacterial agents. Silver nanoparticles (AgNPs) showed a good antibacterial activity and the green synthesis of silver nanoparticles is considered a safe method. In this study AgNPs were biologically synthesized from mycelial free filtrate of an Egyptian mushroom Agrocybe cylindracea. The shift in reaction color from pale yellow to dark brown served as a primary indication for the creation of nanoparticles. The production of AgNPs was further confirmed using UV-visible spectrophotometer and the synthesis process was optimized at different parameters. The best concentration of silver nitrate, pH and temperature values were 4 mM, 7 and 40 °C, respectively. Transmission electron microscopy (TEM), Fourier transform infrared (FTIR), and Zetasizer analyses were used to investigate the characteristics of the produced nanoparticles. TEM studies showed that the size of synthesized AgNPs was 3.47-13.99 nm. FTIR studies showed the presence of some functional groups (O-H, C-H and C=C) which might be involved in the reduction of silver nitrate to silver ion and stabilization of nanoparticles. Zeta potential of the nanoparticles was-3.57. Antibacterial activity of synthesized AgNPs was demonstrated against different harmful microorganisms; Staphylococcus aureus, Pseudomonas aeruginosa and Klebsiella pneumoniae and the best antibacterial activity was recorded against P. aeruginosa.

Antimicrobial activity of silver nanoparticles

2015

Resistant plant pathogens have emerged against the conventional antibiotics. Alternative to these conventional antibiotics application of eco-friendly nanoparticles is an important strategy to manage plant disease. In the present research antimicrobial activity of silver nanoparticles (AgNPs) i.e. Ag, AgP, AgIB, AgAE and AgBE and antibiotics (Nystatin and Streptomycin) were evaluated against plant pathogens Erwinia carotovora pv. carotovora and Alternaria solani. The experiment was carried out in completely randomized Block Design (CRD) with three replications. The antibiotic Nystatin was used as a standard antibiotic reference in case of antifungal activity while streptomycin in case of antibacterial activity. For the antifungal and antibacterial activity different concentration were prepared as 150ppm, 200ppm and 250ppm and zone of inhibition(mm) for all silver nanoparticles (AgNPs) and antibiotics were prepared and inhibition zone was measured in millimeters(mm). Results revealed that the silver nanopartiles(AgNPs) i.e. AgAE and AgIB showed largest inhibition zone with the tested Erwininacarotovorapv. carotovora where the activity was 14.33mm and 13.13mm respectively followed by AgBE (10.40mm), AgP(10.33) and Ag(7mm) while the reference antibiotic streptomycin produced lowest inhibition zone(5.66mm). In case of Alternaria solani maximum inhibition zones were achieved from silver nanoparticles(AgNPs), AgAE and AgIB where the antifungal activity was 27mm and 24mm followed by AgBE(22.33mm), AgP(21.66mm) and Ag(18.66) while the reference antibiotic nystatin produced minimum inhibition zone(4mm). Further it was noticed that increasing the concentration of silver nanoparticles(AgNPs) significantly (P<0.05) increased the inhibition zones of the test plant pathogen and higher concentration(250ppm) posses strong antimicrobial activity. We can conclude that silver nanoparticles(AgNPs), had maximum inhibitory effect against Erwininacarotovorapv. carotovora and Alternaria solani when compared with the antibiotics

Optimized Synthesis of Small and Stable Silver Nanoparticles Using Intracellular and Extracellular Components of Fungi: An Alternative for Bacterial Inhibition

Antibiotics

Silver nanoparticles (AgNPs) represent an excellent option to solve microbial resistance problems to traditionally used antibiotics. In this work, we report optimized protocols for the production of AgNPs using extracts and supernatants of Trichoderma harzianum and Ganoderma sessile. AgNPs were characterized using UV-Vis spectroscopy and transmission electron microscopy, and the hydrodynamic diameter and Z potential were also determined. The obtained AgNPs were slightly larger using the fungal extract, and in all cases, a quasi-spherical shape was obtained. The mean sizes of AgNPs were 9.6 and 19.1 nm for T. harzianum and 5.4 and 8.9 nm for G. sessile using supernatant and extract, respectively. The AgNPs were evaluated to determine their in vitro antibacterial effect against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. The minimum inhibitory concentration (MIC) was determined, and in all cases the AgNPs showed an antimicrobial effect, with a MIC varying from ...

Comparative antibiogram analysis of AgNPs synthesized from two Alternaria Spp. with amoxicillin antibiotics

2015

From long time ago, the use of silver nanoparticles to control the growth and prevalence of disease causing bacteria is well known. In recent times, bacteria make themselves resistant to varied antibiotics based on their genes present in the plasmids. During the present study, an effective approach was performed to synthesize potent silver nanoparticles (AgNPs) from two mould fungi; Alternaria sp. and Alternaria alternata. The appearance of yellowish brown color in the conical flask suggested the formation of AgNPs. The extract of the fungus culture changed the solution into brownish color during the reaction. The characterization of silver nanoparticles was confirmed by UvVis spectroscopy, Field emission scanning electron microscopy (FESEM). Size of the nanoparticles measured between 20nm to 30nm by FESEM. The synthesized silver nanoparticles were subjected to their characterization by X-ray diffraction (XRD) technique to determine the metallic nature of nanoparticles. The XRD anal...

BIOSYNTHESIS, CHARACTERIZATION AND ANTIMICROBIAL POTENCY OF SILVER NANOPARTICLES FABRICATED FROM

Plants that serve medicinal purposes contain phytoconstituents. These phytoconstituents are responsible for therapeutic uses of them. Recently, nanomedicine have gained global attention in medicine. In this study, a cost effective and eco-friendly technique for biosynthesis of silver nanoparticles using 1mM, 2mM and 5mM AgNO solutions with the extract of a fern described. The silver nanoparticles were characterized using UV Fourier Transform Infra Microscope (SEM), Transmission Electron Mic nanoparticles also were subjected to antimicrobial activities and compared with standard antibiotics. The result showed that the fabricated AgNPs with an avera and polydispersed. The synthesized AgNPs exhibited good antibacterial activity against the bacterial pathogens but showed least activity against the fungus antimicrobial agents.