Isolation and Molecular Identification of Keratinophilic Fungi from Public Parks Soil in Shiraz, Iran (original) (raw)
Related papers
2013
Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Introduction. Keratinophilic fungi are an important group of fungi that live in soil. The aim of this study was to isolate and identify keratinophilic fungi from the soil of different parks in Shiraz. Materials and Methods. A total of 196 soil samples from 43 parks were collected. Isolation of the fungi was performed by hair bait technique. The isolated colonies were identified by morphologic feature of macro- and microconidia and molecular method, using DNA sequence analysis. ITS region of ribosomal DNA was amplified and the PCR products were sequenced. Results. 411 isolates from 22 genera were identified. Fusarium (23.8%),
Isolation of keratinophilic fungi from soil in Isfahan province, Iran
Journal De Mycologie Medicale, 2012
Objective.-To evaluate the epidemiology of keratophilic fungi in Isfahan province, Iran. Material and methods.-The present research has been conducted on soil samples collected from 16 townships of Isfahan province. For isolate geophilic dermatophytes and keratinophilic fungi, the keratin baiting technique has been applied. Results.-Of 800 soil samples examined, 588 (73.5%) keratinophilic fungi were isolated. The present studied recognized 727 isolates including 16 species of 11 genus, as follows: Chrysosporium keratinophilum (31.4%), C. pannicola (16.9%), C. tropicum (15.4%), Microsporum gypseum (12.4%), Chrysosporium spp. (9.9%), C. indicum (7%), Sepedonium spp. (3.3%), Malbranchia spp. (1%), Trichophyton terrestre (0.8%), T. ajelloi and Paecilomyces lilacinus (0.4%), Engyodontium album and Acremonium spp. (0.3%), Curvularia spp., Fusarium spp. and Ulocladium spp. (0.1%). In this study, E. album was isolated for the first time in this country (Iran). The frequency these keratinophilic fungi are discussed in relation to different agents such as soil pH. Conclusion.-This study contributes to the knowledge of keratophilic fungi in Iran.
Isolation of keratinophilic fungi from selected soils of Sanjay Gandhi National Park, Mumbai (India)
Journal de Mycologie Médicale / Journal of Medical Mycology, 2014
Eighty samples were collected from six different sites in the vicinity of Gir Forest National Park and screened for the presence of keratinophilic fungi using hair baiting technique for isolation. Seventy three isolates were recovered and identified. The cultures were identified using macro-and micro morphological features. Their identification was also confirmed by the BLAST search of sequences of the ITS1-5.8S-ITS2 rDNA region against the NCBI/Genbank data and compared with deposited sequences for identification purpose. Ten species of seven genera were isolated, viz. Aphanoascus durus (2.50 %), Aphanoascus fulvuscence (5.00 %), Arthrographis kalrae (2.50 %), Auxarthron conjugatum (1.25 %), Chrysosporium indicum (16.25 %), Chrysosporium tropicum (6.25 %), Chrysosporium zonatum (3.75 %), Chrysosporium state of Ctenomyces serratus (7.50 %), Microsporum gypseum (12.50 %), and Trichophyton mentagrophytes (3.75 %). This study indicates that the soils of Gir Forest National Park may be significant reservoirs of certain keratinophilic fungi.
Keratinophilic fungi from selected soils of Bahrain
Mycopathologia, 2007
Eighty soil samples were collected from various sites of Bahrain and screened for presence of keratinophilic fungi using hair baiting techniques for isolation. Thirty-six isolates were recovered and identified. The cultures were identified using macroand micromorphological features. Their identification was also confirmed by the BLAST search of sequences of ITS1-5.8S-ITS2 rDNA region against the NCBI/Gene bank data and compared with deposited sequences for confirmation. Eight species of five genera were isolated viz. Aphanoascus fulvuscence (8.75%), Aphanoascus punsolae (20.00%), Chrysosporium indicum (2.50%), Chrysosporium tropicum (2.50%), Chrysosporium zonatum (3.75%), Spiromastix warcupii (1.25%), Microsporum gypseum (3.75%), and Trichophyton mentagrophytes (2.50%). In conclusion, our study indicates that keratinophilic fungi do occur in the various soils of Bahrain. Moreover, the narrow diversity and low density of keratinophilic fungi in the investigated soils is expected and is emblematic to other hot arid environments.
Prevalence of keratinophilic fungi in public park soils of Mumbai, India
Microbiology Research, 2012
The parks of Mumbai are frequently visited by local residents every morning and evening. However, there are no reports on the occurrence of keratinophilic fungi in these areas. The purpose of this research was to study the occurrence of keratinophilic fungi in the public parks of Mumbai. One hundred soil samples were collected from five public parks: Kamla Nehru Park, Powai Garden, CD Deshmukh Garden, Five Gardens and Chota Kashmir. Keratinophilic fungi were isolated by the hair baiting technique using human hair as keratin bait. The cultures were identified using macroand micro-morphological features. Identification was also confirmed by the BLAST search of sequences of the ITS1-5.8S-ITS2 rDNA region against the NCBI/Genbank data and compared with deposited sequences. The ability of these fungi to use human hair was also evaluated by release of protein in liquid media. A total of 75 strains of keratinophilic fungi were recovered from 100 (75.0%) soil samples. The isolated fungi wer...
The occurrence of keratinophilic fungi in selected soils of Ladakh (India)
Natural Science, 2010
138 soil samples were collected from various locations in Ladakh, a cold desert in the Himalayan region, India and the samples were screened for the presence of keratinophilic fungi using the hair baiting techniques. 58 isolates were recovered and identified. The cultures were identified based on their macro-and micro-morphological features. A total of six genera and fourteen species were isolated namely Amauroascus kuehnii (0.72%), Aphanoascus keratinophilus (4.34%), Aphanoascus terreus (2.17%), Auxarthron alboluteum (0.72%), Auxarthron conjugatum (0.72%), Chrysosporium articulatum (0.72%), Chrysosporium mephiticum (0.72%), Chrysosporium minutisporosum (2.17%), Chrysosporium siglerae (0.72%), Chrysosporium sp. (1.44%), Chrysosporium tropicum (15.94 %), Chrysosporium submersum (3.62%), Chrysosporium state of Ctenomyces serratus (6.52%) and Geomyces pannorum (1.45%). The present study shows that keratinophilic fungi exist in the cold desert of Ladakh.
Journal of Animal and Plant Sciences, 2012
In order to determine the incidence of dermatophytes and related keratinophilic fungi present in Riyadh soil, eighty soil samples were collected .These samples were screened using hair baiting techniques for isolation. Out of a total 80 samples, 69 (86%) were positive for growth of keratinophilic fungi. Eleven genera and 19 species were isolated and identified, of which Chrysosporium indicum (33.75%) was the most predominant species isolated followed by C.tropicum (26.25%), Aspergillus flavus (17.50%), Microsporum gypseum (13.75%) and Trichophyton terrestre (11.25%). Garden soils, followed by playground soils were found to be the most suitable for fungal growth. Some of the other fungi isolated were C.zonatum , Alternaria alternata, Aspergillus niger, Aspergillus terreus, Fusarium moniliforme,F.solani ,Aphanoascus fulvescens etc. To our knowledge, this appears to be the first report concerning the isolation of keratinolytic fungi M. canis and Trichophyton terrestre from soils of Riy...
DNA sequence analysis of keratinophilic fungi isolated from livestock stables in the East of Iran
Current Medical Mycology, 2020
Background and Purpose: Keratinophilic fungi play an important role in the decomposition of keratinous substances in nature. This capacity induces dermatomycosis in both humans and livestock. The soil of livestock stables can be a reservoir of keratinophilic fungi. Therefore, the present study was conducted to isolate and identify keratinophilic fungi in the soil of the livestock stables located in Qayen, South Khorasan Province, Iran. Materials and Methods: This study was conducted on 62 soil samples collected from livestock stables. The samples were cultured by means of hair bait technique (HBT). The identification of the isolates was performed based on their morphological characteristics and then confirmed by polymerase chain reaction and sequencing of the ITS regions of ribosomal DNA. Results: A total of 118 isolates of 7 species from 5 genera were identified. Aphanoascus verrucosu (n=70, 59.36%) was detected as the dominant keratinophilic fungus, followed by Arthroderma quadrif...
Incidence of Keratinophilic Fungi from Selected Soils of Vidarbha Region of Maharashtra State, India
Journal of Mycology, 2014
One hundred and fifty samples were collected from eleven districts of Vidarbha region of Maharashtra state and screened for the presence of keratinophilic fungi using hair baiting technique for isolation. Seventy-one isolates were recovered and identified. The cultures were identified using macro- and micromorphological features. Their identification was also confirmed by the BLAST search of sequences of the ITS1-5.8S-ITS2 rDNA region against the NCBI/Genbank data and compared with deposited sequences for identification purpose. Thirteen species of eight genera were isolated, namely,Auxarthron conjugatum(2.00%),Chrysosporium indicum(14.00%),Chrysosporium evolceanui(2.66%),Chrysosporium tropicum(4.66%),Chrysosporium zonatum(1.33%),Chrysosporiumstate ofCtenomyces serratus(3.33%),Gymnascella dankaliensis(1.33%),Gymnascella hyalinospora(0.66%),Gymnoascoideus petalosporus(0.66%),Microsporum gypseumcomplex (9.33%),Trichophyton mentagrophytes(2.00%),T. terrestre(3.33%), andUncinocarpus que...
Survey of keratinophilic fungi isolated from city park soils of Pisa, Italy
Mycopathologia, 1998
A survey of geophilic dermatophytes and related keratinophilic fungi isolated from city park soils of Pisa is reported. Twenty-three (48%) soil samples out of 48 were positive by hair baiting. The following species were isolated: Microsporum gypseum (39%), Trichophyton ajelloi (31%), Chrysosporium keratinophilum (14%), T. terrestre (8%), M. fulvum, Ch. luteum, Ch. indicum (5% each) and M. cookei (2%). The presence of the different species is discussed in relation to the risk of fungal skin infections.