Analysis of tunisian Mentha pulegium L. oils from Monastir (original) (raw)

Revue Des Regions Arides, 2007

Abstract

The first aim of this study was to describe in detail the chemical composition of Tunisian Mentha pulegium L. essential oils (EOs) from Monastir. The second aim was to test their antibacterial activity. The essential oils obtained from fresh and dried aerial parts were analysed by using gas chromatography and mass spectrometry (GC-MS). The antimicrobial activity was evaluated against reference strains: Gram-positive as Enterococcus faecalis. Staphylococcus aureus and Micrococcus luteus and Gram-negative represented by Escherichia coli and Pseudomonas aeruginosa All the EOs are dissolved in tween 80. The minimum inhibitory concentrations (MIC) and the minimum bactericidal concentrations (MBC) were determinated by sub-culture at TSA agar plates which were incubated at 3 7°C during 18-24h. Thirty four compounds were identified. All oils were found to be rich in oxygen monoterpens hydrocarbons especially menthol, menthone, 1,8-cineole and pulegone. These oils have a good antimicrobial activity against all bacteria tested; Enterocoque feacalis is the most sensitive.

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