STANDARDIZATION OF PROTOCOL FOR IN VITRO MULTIPLICATION OF ORCHIDS (original) (raw)

The investigation was carried out at College of Agriculture, University of Agricultural Sciences, Dharwad during the year 2010-2011 to find out the effect of different treatment combinations for in vitro multiplication of PLB in terms of number of shoots per explants, length of shoot, rooting percentage, number of roots, etc. Eight treatments and their effects on multiplication and preservation of PLBs were measured based on different parameters. Results revealed that all treatments for all the parameters and among the treatments for proliferation, T5 with BAP at 2.0 mg/l exhibit the maximum number of shoots proliferation, shoot length, fresh weight and dry weight. In case of root proliferation T4 with NAA at 1.5 mg/l exhibited the highest rooting percentage, number of roots and length of roots. T4 with 1/ 2 M.S. + BAP 5 mg/l proved to be the most efficient treatment for in vitro preservation (IVP) in terms of number of PLBs developed, number of plantlets per culture respectively. The maximum PLBs development was found to be highest at 150 days of IVP, thereby reducing trend in PLBs development was visible in subsequent days of preservation. Significant results of IVP were observed in both treatments. Highest cost benefit ratio for shoot proliferation and root proliferation were observed in T5 (1:8.10) and T4 (1:8.29) respectively. Therefore, it is concluded that T4 treatment may be recommended for in vitro multiplication, preservation and regeneration in future use.