Point-of-Care Testing Devices for Heart Failure Analyzing Blood and Saliva Samples (original) (raw)
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2010
A microsystem allowing direct and simultaneous analysis of multiple cardiac biomarkers in blood using an integrated filter chip and silicon nanowire (SiNW) sensor chip is described. The integrated microsystem is composed of the filter chip for plasma separation from blood and the SiNW sensor chip for protein detection. These two chips were fabricated into one via back-to-back integration. The SiNW sensor, spotted with three different antibodies, enabled us to detect three cardiac biomarkers, cTnT, CK-MM and CK-MB, simultaneously. The system is able to attain a low detection limit of 1 pg/ml for the three cardiac biomarkers from 2 µl blood in 45 minutes.
Clinical Chemistry, 2009
Background: For adults with chest pain, the electrocardiogram (ECG) and measures of serum biomarkers are used to screen and diagnose myocardial necrosis. These measurements require time that can delay therapy and affect prognosis. Our objective was to investigate the feasibility and utility of saliva as an alternative diagnostic fluid for identifying biomarkers of acute myocardial infarction (AMI). Methods: We used Luminex and lab-on-a-chip methods to assay 21 proteins in serum and unstimulated whole saliva procured from 41 AMI patients within 48 h of chest pain onset and from 43 apparently healthy controls. Data were analyzed by use of logistic regression and area under curve (AUC) for ROC analysis to evaluate the diagnostic utility of each biomarker, or combinations of biomarkers, in screening for AMI. Results: Both established and novel cardiac biomarkers demonstrated significant differences in concentrations between patients with AMI and controls without AMI. The saliva-based bi...
Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference, 2018
The aim of this work is to present KardiaTool platform, an integrated Point of Care (POC) solution for noninvasive diagnosis and therapy monitoring of Heart Failure (HF) patients. The KardiaTool platform consists of two components, KardiaPOC and KardiaSoft. KardiaPOC is an easy to use portable device with a disposable Lab-on-Chip (LOC) for the rapid, accurate, non-invasive and simultaneous quantitative assessment of four HF related biomarkers, from saliva samples. KardiaSoft is a decision support software based on predictive modeling techniques that analyzes the POC data and other patient's data, and delivers information related to HF diagnosis and therapy monitoring. It is expected that identifying a source comparable to blood, for biomarker information extraction, such as saliva, that is cost-effective, less invasive, more convenient and acceptable for both patients and healthcare professionals would be beneficial for the healthcare community. In this work the architecture and...
Point-of-Care Testing for Multiple Cardiac Markers Based on a Snail-Shaped Microfluidic Chip
Frontiers in Chemistry, 2021
Existing methods for detecting cardiac markers are difficult to be applied in point-of-care testing (POCT) due to complex operation, long time consumption, and low sensitivity. Here, we report a snail-shaped microfluidic chip (SMC) for the multiplex detection of cTnI, CK-MB, and Myo with high sensitivity and a short detection time. The SMC consists of a sandwich structure: a channel layer with a mixer and reaction zone, a reaction layer coated with capture antibodies, and a base layer. The opening or closing of the microchannels is realized by controlling the downward movement of the press-type mechanical valve. The chemiluminescence method was used as a signal readout, and the experimental conditions were optimized. SMC could detect cTnI, CK-MB, and Myo at concentrations as low as 1.02, 1.37, and 4.15. The SMC will be a promising platform for a simultaneous determination of multianalytes and shows a potential application in POCT.
Microfluidic‐integrated biosensors: Prospects for point‐of‐care diagnostics
There is a growing demand to integrate biosensors with microfluidics to provide miniaturized platforms with many favorable properties, such as reduced sample volume, decreased processing time, low cost analysis and low reagent consumption. These microfluidics-integrated biosensors would also have numerous advantages such as laminar flow, minimal handling of hazardous materials, multiple sample detection in parallel, portability and versatility in design. Microfluidics involves the science and technology of manipulation of fluids at the micro-to nano-liter level. It is predicted that combining biosensors with microfluidic chips will yield enhanced analytical capability, and widen the possibilities for applications in clinical diagnostics. The recent developments in microfluidics have helped researchers working in industries and educational institutes to adopt some of these platforms for point-of-care (POC) diagnostics. This review focuses on the latest advancements in the fields of microfluidic biosensing technologies, and on the challenges and possible solutions for translation of this technology for POC diagnostic applications. We also discuss the fabrication techniques required for developing microfluidic-integrated biosensors, recently reported biomarkers, and the prospects of POC diagnostics in the medical industry.
Micromachines
Point-of-Care (POC) diagnostics have gained increasing attention in recent years due to its numerous advantages over conventional diagnostic approaches. As proven during the recent COVID-19 pandemic, the rapidity and portability of POC testing improves the efficiency of healthcare services and reduces the burden on healthcare providers. There are hundreds of thousands of different applications for POC diagnostics, however, the ultimate requirement for the test is the same: sample-in and result-out. Many technologies have been implemented, such as microfluidics, semiconductors, and nanostructure, to achieve this end. The development of even more powerful POC systems was also enabled by merging multiple technologies into the same system. One successful example is the integration of microfluidics and electronics in POC diagnostics, which has simplified the sample handling process, reduced sample usage, and reduced the cost of the test. This review will analyze the current development o...
Biosciences, Biotechnology Research Asia, 2015
The aim of the study was the development of a biosensor for prediction and early detection of diseases, including myocardial infarction, based on the analysis of human saliva composition with implanted biosensor devices. For the implementation of the stated objective a device enabling real-time detection of small concentrations of proteins in specific liquid mediums was developed. An evaluation of biosensor sensitivity was performed experimentally with cardiac marker-C-reactive protein, which demonstrates an increase of concentration in all non-specific inflammatory reactions, including myocardial infarction and other acute cardiovascular accidents. A receptor layer was developed based on monoclonal antibodies, enabling specific binding of C-reactive protein, generating lateral strain in the layer. The feasibility of C-reactive protein detection in fluid flow using microcantilever sensors was demonstrated. To allow the possibility of sensor device implantation into oral cavity for continuous monitoring and early detection of diseases based on human saliva composition, a concept and prototype of microcapsule transducer was developed. The transducer constitutes a microaspirator pump with the feature of operator chamber rinsing, which provides circulation of biological fluid samples in the implanted device.