Estimation of Alosetron Hydrochloride in Tablet Dosage Form by RP-HPLC (original) (raw)

2014

Abstract

A simple, precise, rapid and accurate RPHPLC method was developed for the estimation of Alosetron Hydrochloride (AST) in tablet dosage forms. Waters Spherisorb ® 5μm CN, 250x4.6 mm, column with 5 μm particle size and the Mobile Phase consisting of 0.01M Ammonium Acetate in water adjusting the pH-3.2 with Methanol & Tetra Hydro Furan in ratio of 700:240:60 v/v & mobile phase used as a diluent in the gradient mode. The flow rate was 1.0 ml/min and the effluents were monitored at 295 nm. The retention time was 11.570 min and the detector response was linear in the concentration range of 56.1673.2 μg/mL for AST successively. The respective linear regression equation being Y= 27228.755x + 221036.4041 for AST. The Limit of Detection (LOD) & The Limit of Quantification (LOQ) was found to be 0.1 & 0.3 μg/mL for AST. The percentage assay of AST was 99.93%. The method was validated by determining its accuracy, precision and system suitability. The results of the study showed that the proposed...

Ravi Pulla hasn't uploaded this paper.

Let Ravi know you want this paper to be uploaded.

Ask for this paper to be uploaded.