Axon Specificity (original) (raw)

2018

Abstract

Illustration of absent dendrites at the distal and proximal readout position inside MFC microchannels. Representative example of Ctrl Mock iPSC-derived human spinal motor neurons matured over 3 weeks in MFCs showing IF immunostainings for the dendritic marker MAP2 (in pink) and the motor neuron marker SMI32 (turquoise). Note the absence of any MAP2-positive neurites inside channels at either the distal and proximal site where the movie acquisition was performed. Only a few MAP2-positive ramifications were reaching out from the open proximal field to the vicinity of proximal channel entries but remained outside. Scale bar = 10µm.

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