Amaranth as a natural food colorant source: Survey of germplasm and optimization of extraction methods for betalain pigments (original) (raw)
Related papers
Amaranthus Betacyanin Pigments Applied in Model Food Systems
Journal of Food Science, 1999
Amaranthus betacyanin pigments and commercial colorants were evaluated to determine color characteristics and stability at different temperatures in jelly, ice cream, and a model beverage. The betacyanin exhibited brighter color characteristics than red radish anthocyanin, with similar color stability during 20-wk storage (≤ 14 °C) or during the initial 4-wk storage (≤ 25 °C), but was less stable than red radish anthocyanin at 37 °C. The betacyanin was not as stable as synthetic Food, Drug, and Cosmetics Red No. 3 under most storage conditions. Ascorbic acid had a protective effect on Amaranthus pigments. Excessive sucrose (≥ 16%) decreased stability of the pigments. The betacyanin tested may be a feasible natural colorant for jelly, higher pH beverages, and ice cream under selected conditions.
Characterization and application of betalain pigments from plants of the Amaranthaceae
Trends in Food Science & Technology, 2005
The colored vegetative tissues of plants in the family Amaranthaceae contain various betalain pigments and are often produced in high biomass, and therefore, attract interest as potential alternatives to the well-known betalains from beet roots. We attempted a comprehensive systematic study, including selection of betalain-producing genotypes and species, extraction, identification and quantification of betalains, determination and evaluation of physicochemical properties and antioxidant activity; and pigment utilization in certain food systems. The results demonstrated high potential for Amaranthus pigments for use as natural food colorants.
Identification and Distribution of Simple and Acylated Betacyanins in the Amaranthaceae
Journal of agricultural and food chemistry, 2001
Red-colored plants in the family Amaranthaceae are recognized as a rich source of diverse and unique betacyanins. The distribution of betacyanins in 37 species of 8 genera in the Amaranthaceae was investigated. A total of 16 kinds of betacyanins were isolated and characterized by HPLC, spectral analyses, and MS. They consisted of 6 simple (nonacylated) betacyanins and 10 acylated betacyanins, including 8 amaranthine-type pigments, 6 gomphrenin-type pigments, and 2 betanintype pigments. Acylated betacyanins were identified as betanidin 5-O--glucuronosylglucoside or betanidin 6-O--glucoside acylated with ferulic, p-coumaric, or 3-hydroxy-3-methylglutaric acids. Total betacyanin content in the 37 species ranged from 0.08 to 1.36 mg/g of fresh weight. Simple betacyanins (such as amaranthine, which averaged 91.5% of total peak area) were widespread among all species of 8 genera. Acylated betacyanins were distributed among 11 species of 6 genera, with the highest proportion occurring in Iresine herbstii (79.6%) and Gomphrena globosa (68.4%). Some cultivated species contained many more acylated betacyanins than wild species, representing a potential new source of these pigments as natural colorants.
Betalains: Natural plant pigments with potential application in functional foods
LWT - Food Science and Technology, 2015
Betalains are plant derived natural pigments that are presently gaining popularity for use as natural colorants in the food industry. The growing interest of consumers in the aesthetic, nutritional and safety aspects of food has increased the demand for natural pigments such as betalains to be used as alternative colorants in food products. Although betalains from red beetroot are one of the most widely used food colorant, betalains are not as well studied as compared to other natural pigments such as anthocyanins, carotenoids or chlorophylls. This paper reviews the pharmacological properties, such as antioxidant, anti-cancer, anti-lipidemic and antimicrobial activity of betalains derived from sources such as red beetroot, amaranth, prickly pear and red pitahaya, for potential application as functional foods.
Journal of Food Science and Technology, 2018
Natural colorants are important alternatives to synthetic colorants. They are considered harmless and positively affect biological activities owing to their antioxidant potential. The present study deals with the assessment of the extraction processes and the effects of pH (1.0, 3.0, and 5.0), extraction media (water and 50% ethanol) and storage condition (ambient and refrigeration) on betacyanin content, color values, as well as degradation kinetics of total betacyanins in red amaranth. Betacyanin content was more stable at higher than at lower pH. The degradation rate constant (K) was higher and the half-life (t 1/2) was lower at ambient temperature compared to refrigeration temperature. Betacyanin degradation was higher at ambient temperature (30 ± 2°C) than at refrigeration temperature (4°C). The pH, storage time, and temperature affected the stability of the color attributes. Therefore, this work suggests that water and lower temperature (4°C) could be applied to extract more betacyanins from red amaranth and betacyanins might be used as an alternative to synthetic color.
Production and Properties of Spray-dried Amaranthus Betacyanin Pigments
Journal of Food Science, 2000
ABSTRACT: Amaranthus betacyanin extracts were spray-dried using a range of maltodextrins [10-25 dextrose equivalent (DE)] and starches (native/modified) as carrier and coating agents at 5 inlet/outlet air temperatures and 4 feed solid contents. Higher inlet/outlet air temperatures caused greater betacyanin loss during spray drying, and affected slightly the pigment stability during storage. Adding maltodextrins and starches significantly reduced the hygroscopicity of the betacyanin extracts and enhanced storage stability. The 25 DE/10 DE mixed powders provided a longer predicted half-life (63.6 wk) compared to the 25 DE and the 10 DE powders separately. The best dried pigment-containing powder made was superior to commercial red beet powder in physical properties.
Critical Reviews in Food Science and Nutrition, 2000
Pigments are present in all living matter and provide attractive colors and play basic roles in the development of organisms. Human beings, like most animals, come in contact with their surroundings through color, and things can or cannot be acceptable based on their color characteristics. This review presents the basic information about pigments focusing attention on the natural ones; it emphasizes the principal plant pigments: carotenoids, anthocyanins, and betalains. Special considerations are given to their salient characteristics; to their biosynthesis, taking into account the biochemical and molecular biology information generated in their elucidation; and to the processing and stability properties of these compounds as food colorants.
HPLC Characterization of Betalains from Plants in the Amaranthaceae
Journal of Chromatographic Science, 2005
HPLC characterization of reversed-phase (RP) high-performance liquid chromatography (HPLC) has been widely used in separation and identification of plant pigments. An effective RP-HPLC-based method is established to systematically isolate, identify, and quantitate the betalain pigments in the plants of 37 species of eight genera in the Amaranthaceae. A total of 16 betacyanins and three betaxanthins are characterized mainly using the RP-HPLC method and also with the aid of mass spectroscopy. The identified betacyanins include eight amaranthine-types, six gomphrenintypes, and two betanin-types. They are also divided into six simple (nonacylated) betacyanins and 10 acylated betacyanins. Acylated betacyanins are identified as betanidin 5-O-bglucuronosylglucoside or betanidin 6-O-b-glucoside acylated with ferulic, p-coumaric, or 3-hydroxy-3-methylglutaric acids. Three betaxanthins were separated from Celosia species in the Amaranthaceae and identified to be immonium conjugates of betalamic acid with dopamine, 3-methoxytyramine, and (S)tryptophan; the latter two are found to be new betaxanthins from plants.
Spectroscopic screening of assorted pigmented vegetables and fruits for anthocyanins
Anthocyanins is a ubiquitous water-soluble, vacuolar class of pigments that depending on their pH confers red, blue, purple and black color to tissues of higher plants such as leaves, stems, roots, flowers, fruits and bracts [32,33]. They accumulate in the vacuoles of epidermal or sub epidermal cells as an aqueous solution. More so, these pigments have gained wide acceptance because humans and animals have consumed pigmented plants (fruits and vegetables) from the beginning of time without any reported detrimental results and thus their widely adopted use as substitutes for synthetic colors which obviously cannot be subjected to such extensive consumption. This study screened for the presence of anthocyanins in five selected pigmented vegetables and fruits: beetroot (Beta vulgaris), red onions (Allium cepa), red cabbage (Brassica oleracea var. capitata f. rubra), eggplant (Solanum melongena) and red dodo (Amaranthaceae). Beet root, red dodo, red cabbage, egg plants, red onions were ...
Betalains in the era of global agri-food science, technology and nutritional health
Phytochemistry Reviews, 2008
Natural pigments from plants are of growing interest as substitutes for synthetic dyes in the food and pharmaceutical industry and they increase their added value if they possess positive effects on health. These pigments can be added as such if they are in the legal authorized lists of additives or can be added as phytochemical-enriched plant extract achieving the original product, which has received it, the new nomenclature of functional food. In this way, we comprise on this review a wide point of view of a group of natural pigments known as betalains. From a chemical point of view, betalains are ammonium conjugates of betalamic acid with cyclo-DOPA (betacyanins, violet) and aminoacids or amines (betaxanthins, orange or yellow), which are compounds present in our diet. Besides and taking into account that one type of betalain, betanin is approved as food colorant (E-162) by the European Union and that enlarges the specific weight of these compounds in the diet, we have evolved an overview from the biosynthesis, technology and promoting production, industrial uses as pigments up to physiological and nutritional biovailability or biological and health-promoting properties of betalains for accessible information to industrials, researchers and consumers.