Optimization of Solid-State Culture Media for Production of Enzyme Extract Suitable for Chlorogenic Acid Extraction (original) (raw)

Abstract

Introduction. The chlorogenic acid (ClA) presents anti-oxidant, anti-viral, anti-bacterial and anti-fungical activities 1 . This compound is hydrolyzed by chlorogenate esterase enzyme activity (CEA) 2 . The aim of this work was to determine optimal culture conditions for production of enzymatic extract that presents pectinase (PecA) and xylanase (XylA) activities required for ClA extraction from coffee pulp (CP) and avoiding the production of CEA. Material and methods. Assays were realized in fermentation columns with a mixture of 1/3 of CP, 1/3 of olive cake and 1/3 sugarcane bagasse (mesh 16). The culture medium was (100g SS): maltose, 3.5 g; oligoelements solution 0.55 ml. The support/substrate (SS) was inoculated with 1×10 8 spores/g SS, 60% humidity at 2 VKgM at 35ºC. A central composite design was designed with the software STATISTICA 14 th version. The assays factors were the concentrations of sucrose, (NH 4) 2 SO 4 and (NH 4) 2 HPO 4 . The response variables were PecA, XylA ...

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