Mixture Design and Response Surface Methodology for Improving Enzymatic Extraction of Chlorogenic Acid (original) (raw)

Abstract

Introduction. The chlorogenic acid (ClA) has various pharmacological applications such as anti-oxidant, anti-viral, anti-bacterial and anti-fungical 1 . Enzymatic extraction of ClA is selective and harmless to the environment compared with other extraction methods, obtaining a natural label product. The aim of this work was to determine the effect of depolymerizing enzyme activities as pectinase (PecA), xylanase (XylA) and cellulase (CellA) on the extraction of ClA from coffee pulp (CP), using an mixture and response surface methodology. Material and methods. Assays were realized with 5 g of dried coffee pulp (mesh 100), 15 ml of water and 5 ml of phosphate buffer 100 mM at pH 5. The concentrations used (U/g dried CP) were 0 – 200 of each enzyme PecA, XylA and CellA. Enzymatic extraction was performed at 40°C and 100 rpm during 8 h. The mixtures and surface methodology was designed with the software STATISTICA 14 th version. Results. Table 1 shows the results of each enzyme extract ...

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