Evaluation of Moulds Contamination of Cereals and Legumes Sold in Dutsinma Metropolis and Their Aflatoxin Production Potential (original) (raw)
2018
Abstract
Contamination of food materials by aflatoxigenic moulds put the health and well-being of consumers of such food materials at risk of hepatocellular carcinoma. The aim of this study was to isolate and identify moulds capable of producing mycotoxins from maize millet, guinea corn, groundnut and groundnut cake sold to consumers within Dutsinma metropolis, Katsina State of Nigeria. Fungal isolation was done using the direct plating of serially diluted samples of the cereals and legumes, and direct plating technique of surface-sterilized samples of same on potato dextrose agar. Fungi colonies were purified by sub-culturing on fresh potato dextrose agar. The Isolates were identified by comparison of colony morphology and microscopic characteristics with standard reference guide for identification. Determination of aflatoxin producing potential of fungal isolates was carried out using fluorescence under UV-light at 365nm. A total of 124 fungi were isolated and identified. Fifty five (55) o...
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