Oxidative and Epigenetic Changes and Gut Permeability Response in Early-Treated Chickens with Antibiotic or Probiotic (original) (raw)
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2011
The objective of the present study was to evaluate the effect of two probiotic strains Lactobacillus fermentum CCM 7158 and Enterococcus faecium M 74 given to the drinking water on the some parameters of energy profile (cholesterol, triglycerides, total protein, glucose) and antioxidant status (total antioxidant status – TAS, albumins, bilirubin) of female broiler chickens. The experiment was conducted on hybrid Hybro (n=180). The feeding period lasted 42 days. Experimental chickens of E1 group received a probiotic preparation in drinking water with concentration of 1.10 colony forming units (CFU) of L. fermentum CCM 7158 in 1 g of nutrient medium and experimental chickens of E2 group concentration of 2.10 CFU of E. faecium M-74 in 1 g of nutrient medium. The control group of animals received water without any additives. TAS in both probiotic groups was significantly increased. No significant differences were found in other parameters.
Immune Parameters in Chickens Treated with Antibiotics and Probiotics during Early Life
Animals
The aim of the study was to compare the effect of the administration of antibiotics or probiotics on chickens in their first week of life, on selected parameters of humoral and cellular immunity, and on the bursa of Fabricius and spleen indices. The experiment was carried out on 90 one-day-old male broilers. The control group received no additive in the drinking water; the group GP received a probiotic providing Enterococcus faecium and Bacillus amyloliquefaciens; and the group GA received 10% enrofloxacin in the drinking water on the first five days of life. Administration of the antibiotic enrofloxacin or a probiotic containing E. faecium and B. amyloliquefaciens strains to chickens in their first week of life exerts pronounced immunomodulatory effects on humoral and cellular defense mechanisms in these birds. The changes in the subpopulations of B and T cells immediately following early administration of enrofloxacin or the probiotic were not observed at the age of 35 days. Early...
Poultry Science, 2016
The current study investigated the effects of Lactobacillus acidophilus and Bacillus subtilis, used as probiotics, on the microflora, morphology, and morphometry of the gut in organic laying hens. The birds (180 Hy-Line laying hens) were divided into 3 homogenous groups and received a pre-deposition diet from 16 to 20 wk of age and a deposition diet for the remaining 7 months of the experiment. The control group (CTR) was fed a corn-soybean cake-based diet, the second group (L) received the same diet supplemented with 0.1% of L. acidophilus while in the third group (B) the basal diet was supplemented with 0.05% of B. subtilis. At 18 wk of age (T1) and at 5 (T2) and 7 months (T3) from the beginning of deposition, 9 subjects per group were humanely killed for microbiological, morphological and morphometric analyses of the intestinal tract. The 2 probiotic-supplemented diets increased Lactobacillus spp. and Bifidobacterium spp. counts compared with the CTR diet. The lowest viable counts of E. coli, coliforms and staphylococci were observed in the L group (P < 0.001). Clostridium spp. decreased (P < 0.001) in both L and B subjects. The probiotic supplementation appeared to affect the intestinal microbial population, promoting the presence of beneficial bacteria such as Lactobacillus spp. and Bifidobacterium spp. and reducing potential harmful bacteria such as E. coli, clostridia and staphylococci. Morphological and morphometric analyses did not reveal substantial differences among groups. At T3, the plasma cell infiltrate in the villi of the CTR hens was more severe than that observed in the L and B groups (P = 0.009).
Journal of Animal Physiology and Animal Nutrition, 2018
The purpose of this study was to verify the ability of a probiotic in the feed to maintain the stability of the gut microbiota in chickens after antibiotic therapy and its association with growth performance. One thousand six hundred twenty 1-day-old Cobb male were housed in floor pens (36 pens, 45 birds/pen) and were fed corn-/ soya bean meal-based diets supplemented with or without probiotic (Bacillus subtilis) during the entire rearing phase. From 21 to 24 days of age (three consecutive days), the chickens were submitted to antibiotic therapy via drinking water (bacitracin and neomycin) in order to mimic a field treatment and induce dysbiosis. Growth performance was monitored until 42 days of age. At 2, 4 and 6 days after antibiotic therapy, three chickens from each pen were euthanized and the contents of the small intestine and caeca were collected and pooled. The trial was conducted with four treatments and nine replicates in a 2 × 2 factorial arrangement for performance characteristics (with and without probiotic × with and without antibiotic therapy); for the intestinal microbiota, it was in a 2 × 2 × 3 factorial arrangement (with and without probiotic × with and without antibiotic therapy × 2, 4 and 6 days after the antibiotic therapy) with three replicates per treatment. Terminal restriction length polymorphism (T-RFLP) analysis showed that the structure of gut bacterial community was shaped by the intestinal segment and by the time after the antibiotic therapy. The number of 16S rDNAs copies in caecum contents decreased with time after the therapeutic treatment. The antibiotic therapy and dietary probiotic supplementation decreased richness and diversity indexes in the caecal contents. The improved performance observed in birds supplemented with probiotic may be related to changes promoted by the feed additive in the structure of the intestinal bacterial communities and phylogenetic groups. Antibiotic therapy modified the bacterial structure, but did not cause loss of broiler performance.
Effects Of Locally-Made Probiotics on Drinking Water on the Performance of Chickens
Biosaintifika: Journal of Biology & Biology Education
This study investigated the effects of locally-made probiotics on the performance of Kabir and Starbro broilers. The birds were randomly assigned to four treatments, and their body weight, weight development, feed consumption, feed conversion efficiency, dressing percentage, livability rates, and income over feed and chick cost were measured. The results showed a significant interaction between the breed of chicken and the type of probiotics after 21 days of feeding in terms of body weight, body weight gain, feed consumption, and feed conversion efficiency. However, no significant interaction was observed at 35 days of feeding. The use of locally produced probiotics in drinking water improved the dressing percentage of both breeds of chicken, overall body weight gain, and overall feed consumption compared to the control group. Livability rates were not significantly affected by the inclusion of different locally-made probiotics in drinking water. Net income per chicken was marginall...
Effects of prebiotics on intestinal histo-morphometry and gut microflora status of broiler chickens
Indian Journal of Animal Research, 2018
A feeding trial was conducted to assess the effect of prebiotics on intestinal histo-morphometry and gut health status of broiler chickens. A total number of 240 day-old broiler chicks were randomly assigned to six dietary treatment groups having five replicates each with eight birds per replicate for six weeks. A basal corn-soybean diet was formulated that served as control (T1). The rest five test dietary treatments were prepared by adding antibiotic (bacitracin methylene di-salicylate-BMD at 0.002% of diet (T2), prebiotics i.e., mannan oligosaccharides (MOS) at 0.1% or 0.2% (T3 and T4) and fructo oligosaccharides (FOS) at 0.1% or 0.2% (T5, T6 respectively) with basal diet. Total anaerobes counts were significantly (P LESS THAN 0.05) reduced at 21 and 42 d at ileum in T2, T3 and T4 groups whereas, coliforms count were reduced (P LESS THAN 0.05) in T2 and T3 groups. However, lactic acid bacteria (LAB) count were significantly (P Less than 0.05) increased in T3 and T4 groups but lac...
Frontiers in Veterinary Science
In commercial poultry production, chickens are reared under intensive conditions, which may allow infections to spread quickly. Antibiotics are used at sub-therapeutic doses in livestock and poultry feed to prevent diseases and improve productivity. However, restrictions on the use of antibiotics at sub-therapeutic concentrations in livestock feed due to growing concerns of antimicrobial resistance (AMR), together with antibiotic residues in meat and eggs has prompted poultry researchers and feed producers to look for viable alternatives. Thus, there is increasing interest in developing natural alternatives to in-feed antibiotics to improve chicken productivity and health. Probiotics, specifically from the genusBacillushave proven to be effective due to their spore-forming capabilities. Furthermore, their ability to withstand heat during feed processing and be stored for a long time without losing viability as well as their potential to function in the acidic medium of the chicken g...
Journal of Applied Pharmaceutical Science, 2019
The aim of this work was to investigate the efficacy of a couple of probiotic lactic acid bacterial isolates, Lactococcus lactis ssp. lactis and Lactobacillus plantarum added separately or in combination to broiler diets. The experimental treatments received a basal diet with 22.4% protein and 3,160 kcal/kg. Two hundred and ten 1-day-old Hubbard broilers were allocated in seven experimental groups as follows: Control group and six groups treated by both probiotic strains in drinking water with intended final concentration of 10 9 cfu/ml and/or 10 12 cfu/ml separately or in combination for a period of 42 days and tested on scheduled intervals. Treatment effects on performance of broilers (organs weights) as well as certain serum constituents were determined. The composition of cecal microflora was also evaluated. Probiotic supplementation had no significant effect but some organs had relative weights slightly higher compared to control. However, the relative weight of the thyroid, spleen, and pancreas was significantly increased. Broilers that received both types of probiotic strains separately or in combination had significant decreases (p < 0.05) in both serum alanine aminotransaminase level and malondialdehyde along with a significant increase (p < 0.05) in total antioxidant capacity compared to control. The microbiological analysis indicated that the lactic acid bacterial population boosted predominantly. The total coliform and Salmonella counts were significantly reduced and/or totally eliminated in broiler groups supplemented with probiotics. In conclusion, this study showed that both probiotic lactic acid bacterial strains can be considered as a nutritional source for broiler chickens.