The Isolation and Identification of Active Compound of Dendrophthoe praelonga (Blume) Miq. Extract Against Breast Cancer Cells (MCF-7) (original) (raw)
Related papers
Veterinary World, 2022
Background and Aim: The flavonoids from mistletoe are thought to have antimicrobial action. This encouraging finding supports the benefits of medicinal plants as a substitute for synthetic antimicrobials, thus promoting healthy lifestyles. In contrast, it is known that the use of topical drug formulations made from flavonoids of mistletoe (Dendrophthoe pentandra (L.) Miq. Loranthaceae) with Indonesian name, Benalu duku (BD) is required in skin cell irritation. This study aimed to assess the toxic effects of the flavonoid substances of BD, as an initial screening. Materials and Methods: A myeloma cell line was cultured in Roswell Park Memorial Institute medium, and the Baby Hamster Kidney clone 12 (BHK21) cell line was cultured in Dulbecco's Modified Eagle's Medium from stock (±9 × 107 cells/mL), and 1.2 mL of culture were distributed into each well of a microtiter plate. Subsequently, 0.2 mL of serially diluted flavonoid compounds (0.5–3 μg/mL) were added to 12 wells for each concentration, as trial groups (including control groups), followed by a 2-day incubation. Observations were performed based on the cytopathic effect (CPE) using an inverted microscope at a magnification of 100×. Results: Cytopathic effect was detected on the microtiter plate wells for the groups of myeloma and BHK21 cells at a flavonoid concentration of 0.5 μg/mL–3 μg/mL. Conclusion: Flavonoid compounds from BD were safely used for topical treatment of cancer cells at a concentration <2.491 μg/mL, whereas for non-cancerous cells, a concentration <2.582 μg/mL was sufficient (p < 0.05).
Molecules
A new, rapid, sensitive and selective liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) method was developed to determine the content of flavonoid aglycones and phenolic acids in mistletoe berries (Viscum album L.) harvested from six different Polish host trees. Additionally, the total phenolic content (TPC) and total flavonoid content (TFC) as well as an antioxidant and antiproliferative activity were evaluated for the first time. The plant material was selectively extracted using ultrasound assisted maceration with methanol/water (8:2) solution. The obtained TPC and TFC results varied from 7.146 to 9.345 mg GA g −1 and from 1.888 to 2.888 mg Q g −1 of dry extracts, respectively. The LC-ESI-MS/MS analysis demonstrated the highest content of phenolic acids in mistletoe berries from Populus nigra 'Italica' L. and flavonoid aglycones in mistletoe berries from Tilia cordata Mill. (354.45 µg and 5.955 µg per g dry extract, respectively). The moderate antioxidant activity of investigated extracts was obtained. The studies revealed that the examined extracts decreased the proliferation of human colon adenocarcinoma cells line LS180 in a dose-dependent manner without cytotoxicity in the human colon epithelial cell line CCD 841 CoTr. Moreover, the obtained results suggest considerable impact of polyphenols on the anticancer activity of these extracts.
Breast cancer is the most common cancer among women. It is estimated that one of eight women will be diagnosed with breast cancer in women. The betel leaves (Piper betle L.), madagascar prewinkle (Catharanthus roseus [L] G.Don), mango parasite (Dendropthoe petandra L.) and white saffron (Curcuma mangga Val.) have been reported to exhibit antioxidant, and antimutation that suggested the chemopreventive potential against various cancer including breast cancer. This research was conducted to investigate anticancer activity of P. betle, C. roseus, D. petandra and C. mangga extracts on breast cancer cell line T47D, and antioxidant activity. The anticancer activity was determined with MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay. The antioxidant activity was determined by using in vitro assay of 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity. P. betle , C. roseus extracts were able to inhibit T47D cell proliferation with IC 50 55.2 µg/ml, 26.22 µg/ml and D. petandra, C. mangga extracts with IC 50 728.05 µg/ml, 404.76 µg/ml, while DPPH scavenging activity (IC 50 ) on P. betle, C. roseus, D. petandra, C. mangga extracts were respectively 5.49 µg/ml, 102.96 µg/ml, 4.74 µg/ml, 277.79 µg/ml. P. betle and D. petandra extracts are more active antioxidant compared to C. roseus and C. mangga.
2017
Indonesia has so many kind of benalu (mistletoe), one of them wich has potential anticancer activity is benalu mangga (Dendrophthoe petandra). This research aims to determine the profile of anticancer activity of benalu mangga leaves extract from several locations in Indonesia against T47D cell line using invitro technique. The profile of anticancer activity can be used as one of the Requirement developed to be fitofarmaka. The separation of the active compound from leaves mistletoe mango do with ultrasonic maseration extraction method using a solvent of ethanol 96%. Each extract derived from 5 locations i.e. Kediri East Java, Pekalongan Central Java, Denpasar Bali, Lampung Sumatra and Bulungan Kalimantan at test level of toxicity against the cell line T47D breast cancer by MTT method Assay. Test statistic one way analysis of variance (Anova) with software SPSS version 16.0 used assess whether there is a difference significantly anticancer activity (IC50) leaf extract mistletoe mang...
Bioactivities Evaluation of Indonesian Mistletoes (Dendrophthoe pentandra (L.) iq.) Leaves Extracts
2012
Mistletoes or benalu in bahasa Indonesia is a semi-parasitic plant that also known as medicinal plant. It used in traditional/alternative medicine such as for for cough, diabetes, hypertension, cancer, diuretic, smallpox, ulcer, skin infection and after childbirth treatment. There are many species of mistletoes in Indonesia. Dendrophthoe pentandra (L.) Miq. is one of the Indonesian mistletoes species that commonly found grew on many different species of host plant. In this paper we reported in vitro toxicity, antioxidant and antidiabetes activities of MeOH and water extracts of D. pentandra grew on four different host plants (Stelechocarpus burahol, Spondias dulcis, Annona squamosa and Camellia sinensis). Toxicity was measured using brine shrimp lethality test (BSLT). Antioxidant activity was measured using DPPH free radical scavenging assay. Antidiabetes activity was measured using -glucosidase inhibitor assay. The results show that all mistletoes extracts tested (MeOH and water extracts) were non-toxic and show significant antidiabetes activity, whereas for antioxidant activity, only MeOH extracts show significant activity. Therefore, it is suggest that D. pentandra extracts are potential source for natural antioxidant and antidiabetes compounds.
PROCEEDINGS OF THE 4TH INTERNATIONAL SYMPOSIUM ON CURRENT PROGRESS IN MATHEMATICS AND SCIENCES (ISCPMS2018), 2019
Mistletoe, Dendrophthoe pentandra (L.) Miq., was reported to be the most frequently obligate hemiparasite species found in Kampus UI, Depok. Mistletoe has been reported containing bioactive compound metabolites. This research investigated the antioxidant activity of three mistletoe individuals live on different host plants in Kampus UI. The host plants are Mangifera indica, Bauhinia pupurea and Stelechocarpus burahol. Samples extracted with methanol to yield crude extracts and DPPH (1,1-Diphenyl-2-Picrylhydrazyl) method was used to test the antioxidant activity. The results showed that all extract of mistletoe has antioxidant activities with a range IC50 value of 10-25 μg/mL. The HPLC analysis showed that each extract has quite similar chromatogram profile. Meanwhile, the total flavonoid and phenolic compound from all the extract range are 37.64-52.92 g QE/100 g and 349.6-431.6 mg GAE/100 g. These results suggested that mistletoe extracts might have interesting bioactive metabolites as an antioxidant and therefore should be further investigated for isolation and structure elucidation. This research also gives a conclusion that different host plants seem to have almost the same metabolites composition.
SSR Institute of International Journal of Life Sciences, 2020
Background: Viscum articulatum Burm.f. is a hemiparasitic plant belongs to family Viscaceae, which having various traditional medicinal uses. The present work was attempted to determine phytoconstituents and in vitro antioxidant activity of V. articulatum by using DPPH scavenging assay to evaluate their potential as to elucidate pharmacological actions. Methods: The dried plant material powdered administered to soxhlet extraction with petroleum ether, benzene, chloroform, acetone, ethanol and distilled water respectively for 18 h. The condensed extracts were tested for qualitative assessment of phytochemicals. In vitro, antioxidant activity was conducted via DPPH radical scavenging assay. Antioxidant activity was assessed with acetone and ethanolic crude extract to evaluate the free radical scavenging activity (Inhibition (%)/ Scavenging) of V. articulatum at different concentrations of stock solution likes 50, 100, 150, 200, and 250 µg/ml. Results: Phytochemical investigation prominently reveal the presence of phytoconstituents like carbohydrates, cardiac glycosides, proteins, alkaloids, fats, saponin, coumarins, flavonoids, tannins, phenolics, steroids and quinone, which have been responsible for various biological activities. The IC50 value of acetone and ethanol extract of the studied plant was found to be 9.4 and 8.9 respectively, which was comparable with standard ascorbic acid (IC50 = 5.4). Conclusion: The results suggest that V. articulatum exhibits the excellent potential of antioxidant activity that may be beneficial for its medicinal values.
Comparative study concerning mistletoe viscotoxins antitumor activity
Acta Biologica Hungarica, 2013
Viscum album l. (Santalaceae), VA, -a parasitic plant that grows on various trees -, has proved a significant anticancer effect in both experimental studies and clinical trials. The present study assesses the influence of oxidative stress in mistletoe induced cytotoxicity in tumor cells, in relation to classic cytostatic therapy. VA ethanolic extract was administered alone and combined with doxorubicin (chloride), in Swiss female mice previously intraperitoneally (i.p.) inoculated with Ehrlich tumor cells (1×10 6 /animal), that consequently developed Ehrlich ascites carcinoma (EAC). The administered doses were of 50 mg/kg in the 1 st , 3 rd and 6th day for the VA extract, respectively of 2.5 mg/kg in the 1 st and 6 th day for doxorubicin, after tumor cell implantation. Fourteen days after, all mice were euthanized, ascites of the EAC were collected in order to analyze the tumor proliferation parameters, as well as blood samples, in order to evaluate the antioxidant status in plasma. Tumor development was associated with increased activity of plasma enzymes; classic doxorubicin therapy not only prevents the accumulation of ascitic fluid, but as well, significantly reduces the activity of plasma antioxidant enzymes. Furthermore, in association with VA extract, the protective effect is improved. Oxidative changes in Ehrlich tumor cells consisted in decreased catalase activity and amplified xanthine oxidase and peroxidase activities.
Assessment of Antioxidant and Cytotoxic Activities of Extracts of Dendrobium crepidatum
Biomolecules
Dendrobium crepidatum is an epiphytic orchid found in south Asia including Nepal and China. This orchid species is widely used in traditional Chinese medicine (TCM) for the treatment of cancer, diabetes, cataracts, and fever. The objectives of the present research were to assess the antioxidant and cytotoxic properties of its stem’s extracts with the identification of bioactive secondary metabolites. The antioxidant and cytotoxic activities were evaluated using the DPPH (2,2-diphenyl-1-picrylhydrazyl) and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays, respectively, and compounds were identified using GC–MS (gas chromatography and mass spectrometry). Ethanol and acetone extracts scavenged 94.69 ± 0.10% and 93.41 ± 0.86% of DPPH free radicals, respectively. They showed 50% inhibition of DPPH free radicals (IC50) at concentrations of 73.90 µg/mL and 99.44 µg/mL, which were found to be statistically similar to that of ascorbic acid (control). Chloroform extra...
International Journal of Scientific Research in Science, Engineering and Technology, 2022
Because of their ability to neutralise free radicals, phenolic compounds have a variety of biological effects, including anti-inflammatory, antibacterial, and antioxidant characteristics. Antioxidants are essential nutrients that protect the body from the harmful effects of oxidative stress caused by free radicals. A wide range of free radical scavenging antioxidants can be found in a variety of foods. The study's goal was to find out how much total phenolic content, total flavonoids content, antioxidant activity, and phytochemical screening of herbal drugs. Flavonoids are a type of natural polyphenolic chemicals that humans are unable to produce. These compounds have a variety of biological characteristics and act as antioxidants in biological systems. The Folin-Ciocalteau method was used to determine the total phenolic content. Antioxidant properties were determined using 1,1-diphenyl-2- picrylhydrazyl (DPPH). The total phenolic and flavonoid content was determined spectrophotometrically using gallic acid and quercitin as standards. The Herbal drug for the study is purchased from the local markets of Nashik (Maharashtra). 5 percent, 2.5 percent, 1.25 percent, 0.625 percent, 0.3125 percent, and 0.15625 percent concentration samples in water of the herbal medication were prepared for the assessment of antioxidant potential by DPPH assay. As the concentration % rises, so does the antioxidant activity, as well as the overall phenolic and falvonoid content increases. The antioxidant activity is due to the existence of total phenolic and flavonoid content. Conventional methods were used to determine the presence of specific phytochemicals.