Vancomycin as an Antibacterial Agent Capped with Silver Nanoparticles: An Experimental Potential Analysis (original) (raw)
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Advanced pharmaceutical bulletin, 2017
Purpose: Many antimicrobial medications are available to combat infections. However, the indiscriminate use of antibiotics has produced antibiotic resistance in the case of many bacterial pathogens. This study focuses on the development of nanoparticles (NPs) that enhance the in vitro antibiotic activity of vancomycin against multi-drug resistant (MDR) organisms. Methods: Spherical shaped thioglycolic acid-stabilized silver nanoparticles (TGA-AgNPs) were prepared by using a simple chemical reduction method. Then, vancomycin was conjugated to the terminal carboxyl of TGA in the presence of N-Hydroxysuccinimide (NHS) and N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC). Afterwards, the antibacterial activity of these nanoconjugates was examined by using the minimum inhibitory concentration (MIC) assay against MDR bacteria. Results: The rate of vancomycin bound to the AgNPs was 19.6%. The MIC values of vancomycin (Van)-capped AgNPs against tested pathogens were in...
Asian Journal of Pharmaceutical Research and Development
In Recent year's medication obstruction is a quickly developing issue over the whole world in the treatment of infectious diseases. The widespread use of broad-spectrum antibiotics produced antibiotic resistance for many human bacterial pathogens. Anyway right now, nanotechnology research has been engaging more in restorative businesses with various advantages because of the way that surface area to volume proportion of Nanoparticles is quite large. In this exploration work, Silver Nanoparticles (AgNPs) are prepared by using a reducing agent like sodium borohydride and capping agent polyvinylpyrolidine. The AgNPs were set up by the chemical reduction method. Nanotechnology has its own importance it eventually reduce dose and also have a superior bioavailability than larger particles. The prepared AgNPs were subjected to characterization like particle size, X-Ray diffraction. In addition, the AgNPs drawing much interest in account of their powerful antibacterial movement. The an...
Inhibition of the Vancomycin Resistance in Staphylococcus aureus in Egypt Using Silver Nanoparticles
BioMed Research International
Staphylococcus aureus is a major human pathogen that is sometimes resistant to vancomycin. In this study, the prevalence of vancomycin-resistant Staphylococcus aureus (VRSA) was studied. 100 isolates of S. aureus were identified based on biochemical and molecular evidence. The antibiotic susceptibility of the studied isolates was tested against 13 antibiotics by the disc diffusion method that showed 24 vancomycin-resistant isolates. The minimum inhibitory concentrations (MICs) were estimated by the agar dilution method to determine vancomycin intermediate-resistant S. aureus (VISA) and VRSA. The resistance gene cluster (vanA, vanR, vanH, and vanY) was amplified by PCR and then sequenced. Amplification of vanA and vanR genes showed that they are present in 21.4% and 14.3% of VRSA isolates, respectively, whereas none of the studied genes has been detected in VISA strains. A significant antimicrobial effect toward VRSA isolates using silver nanoparticles (AgNPs) synthesized from S. aur...
Biosynthesis of Silver Nanoparticles (AgNPs) has received great attention due to their numerous advantages like being rapid, simple and eco-friendly protocol. In the present study, weused cultures supernatant of pathogenic bacteria and fungi like Staphylococcus aureus 29737 ATCC, Staphylococcus epidermidis 12228 ATCC and Klebsiella pneumoniae 700603 ATCC,as well as cell free filtrate of Fusarium sp. and Aspergillus flavusfor rapid synthesis of silver Nanoparticles.UV-visible spectrometry study revealed the plasma resonance from420 to 440nm.Transmission electron microscope of AgNPs showed the mean average size of most particles was˜11,29.9, 16.8, 23.4 and 23.4 nm for Staphylococcus aureus, Staphylococcus epidermidis and Klebsiella pneumoniae, Fusarium sp. and A. flavusrespectively. The Fourier Transmittance Infrared spectrum (FT-IR) confirmedthe reduction of the silver ions and forming stable and control shaped silver nanoparticles due to the protein component resulting from the enzyme nitrate reductase.Results of this study revealed that the synthesized silver nanoparticles have potential antibacterial activity and were also capable of enhancing the efficiencies of some commercial antibiotics.
Antibiotics
Bacterial drug resistance to antibiotics is growing globally at unprecedented levels, and strategies to overcome treatment deficiencies are continuously developing. In our approach, we utilized metal nanoparticles, silver nanoparticles (AgNPs), known for their wide spread and significant anti-bacterial actions, and the high-dose regimen of lincosamide antibiotic, lincomycin, to demonstrate the efficacy of the combined delivery concept in combating the bacterial resistance. The anti-bacterial actions of the AgNPs and the lincomycin as single entities and as part of the combined mixture of the AgNPs–lincomycin showed improved anti-bacterial biological activity in the Bacillus cereus and Proteus mirabilis microorganisms in comparison to the AgNPs and lincomycin alone. The comparison of the anti-biofilm formation tendency, minimum bactericidal concentration (MBC), and minimum inhibitory concentration (MIC) suggested additive effects of the AgNPs and lincomycin combination co-delivery. T...
2021
Methicillin resistant Staphylococcus aureus (MRSA) has emerged as a major problem worldwide. Strong synergistic action of antibiotics combined with silver nanoparticles (AgNPs) presents a potential solution for this problem. AgNPs was prepared by Gamma- irradiation method. Eighty staphylococcus spp. were obtained out of 45 different clinical specimens; 21(46.6 %) isolates were identified as MRSA and chosen to test their susceptibility to plain AgNPs, β-lactam antibiotics and mixed solution of AgNPs /antibiotics by disc diffusion method and broth microdilution assay for detection of minimum inhibitory concentration (MIC) as well as minimum bactericidal concentrations. All MRSA isolates were full resistant to amoxicillin, ceftazidime, cefotaxime, ceftriaxone, cefepime, while 3(14.2%) were susceptible to meropenem and imipenem with MIC ≤ 20µg/ml. Plain AgNPs showed potential anti-MRSA activity, the range of inhibition zones of AgNPs ranged from 7.5 to 12 mm. Broth microdilution assay r...