Anti-pseudomonal and anti-endotoxic effects of surfactin-stabilized biogenic silver nanocubes ameliorated wound repair in streptozotocin-induced diabetic mice (original) (raw)
Related papers
International Journal of Nanoparticles, 2015
Impaired wound healing is a common complication of diabetes mellitus. Nanofibre can be served as powerful tools in advanced wound care management. The aim of this work is to evaluate the role of antibacterial nanofibres by incorporating silver nanoparticles into celluose acetate nanofibres as wound dressing on excisional wound healing in diabetic mice. Celluose acetate nanofibres were prepared by electrospinning technique. The prepared nanofibres were characterised using scanning electron microscope (SEM), and Fourier-transform infrared (FTIR) spectrophotometer. The antimicrobial activity was tested against Gram-positive and Gram-negative bacteria. A full thickness of the excision wound of circular area 95 mm 2 and 2 mm depth was created. Wound dressed by silver containing nanofibres, the wound closure rate were assessed, skin wounds were processed for histopathological examination Preparation and characterisation of antibacterial 83 and comparing the mechanical properties of healed skin. It was found that, wounds dressed with silver containing nanofibres showed marked increase in collagen production which improve the skin mechanical properties.
Frontiers in Bioengineering and Biotechnology, 2020
Bacterial infections are the key cause of death in patients suffering from burns and diabetic wounds while the use of traditional antibiotics has been growing steadily. Thus, in the present study, we are trying to introduce a paradigm shift strategy to improve chronic wound healing of bacterial infection. To that end, we have biologically synthesized silver nanoparticles (AgNPs) using Arthrospira sp polysaccharides, and evaluated their antibacterial efficacy with their safety pattern. Scanning electron micrographs showed spherical AgNPs coated with algal polysaccharides with an approximate size of 9.7 nm. Treatment of Pseudomonas aeruginosa with the AgNPs (0.5-1 µg/mL) resulted in a significant disruption in P. aeruginosa outer membrane, reduction in biofilm formation, and a significant decrease of production of alginate and pyocyanin along with a concentration-dependent reduction in β-lactamase activity. In addition, at the in vivo level, AgNPs displayed substantial activity to control P. aeruginosa infections in rat skin wounds with significant reduction in in COX-2 enzyme in both rat skin homogenate and serum samples. Furthermore, AgNPs facilitated wound curative in the P. aeruginosa infected model by reducing the hemorrhagic areas number and the infiltrated inflammatory cells. Taken all together, these biogenic nanoparticles showed unique properties in controlling bacterial wound infections and improving the healing process of damaged tissues via its direct and indirect effects.
Silver Nanoparticles for the Rapid Healing of Diabetic Foot Ulcers
International Journal of Medical Nano Research, 2017
Diabetic foot ulcers are one of the major complications of patients with diabetes mellitus. And due to their high susceptibility to microbial infections, are the leading cause of hospitalization and amputation of lower limbs. It has been well studied the antimicrobial properties of silver nanoparticles (AgNPs), therefore their use in biomedicine is a trend. Herein we present for the first time the use of AgNPs for the treatment of diabetic foot ulcers of grade II and III of Wagner classification. Ulcers were treated by topical administration of AgNPs (at 1.8 mg/mL of metallic silver) in addition to conventional antibiotics. In all the cases presented in this study, a significant improvement in the evolution of ulcers was observed upon AgNPs administration. The edges of the lesion reached the point of closure. These results constituted the basis for further studies on the use of AgNPs for the treatment of chronic ulcers from different origins.
Metal nanoparticles and biomaterials: The multipronged approach for potential diabetic wound therapy
Nanotechnology Reviews
Metal nanoparticles have been widely used in the treatment of diabetic wounds owing to their proven antibacterial activity and enhanced wound healing effects. Therefore, in this review, we discuss the use of metal nanoparticles in managing diabetic wounds, mainly silver nanoparticles (AgNPs), gold nanoparticles (AuNPs), and zinc nanoparticles (ZnO nanoparticles), as well as their combination with biomaterials such as chitosan, bacterial cellulose, growth factors, etc. The combination of metal nanoparticles and biomaterials reportedly halts the growth and multiplication of bacterial strains commonly involved in diabetic wounds, including gram-positive (Staphylococcus aureus and Acinetobacter calcoaceticus) and gram-negative bacteria (Pseudomonas aeruginosa, Escherichia coli, and Klebsiella pneumoniae). Furthermore, these combinations have demonstrated enhanced wound healing of diabetic wounds during in vitro and in vivo studies. Additionally, we highlighted the barriers and challenge...
Iraqi Journal of Science
Diabetic foot is a catastrophic complication of diabetes. This study included isolation and identification of three types of bacteria that cause diabetic foot ulcers, fifty-five isolates of Staphylococcus aureus, thirty-five isolates of Acinetobacter baumannii, and thirty isolates of Serratia marcescens. These isolates were obtained from diabetic foot patients at different private clinics in and around Baghdad and Medical City Hospital. The proportion of male patients was greater than females, and it was noted that the age group (51-68 years) was more ages affected by diabetic foot. These isolates showed high resistance to most of the antibiotics used, Staphylococcus aureus was resistant to Penicillin, Tetracycline, and Ciprofloxacin in the percentage of 100 %, 65 %, and 26 %, respectively. Acinetobacter baumannii, showed high resistance to Penicillin, at a percentage of 80%, and Ciprofloxacin at 60%. Serratia marcescens was resistant to most antibiotics that were used in thi...
Materials science & engineering. C, Materials for biological applications, 2018
Microbial contamination along with over expressions of matrix metalloproteinases 2 and 9 impairs wound healing in diabetic patients. Silver-based antimicrobial agents have been successfully used for treating non-healing chronic wounds associated with infection. However, topical application of silver-ion compounds impairs wound healing process. Thus, usage of biogenic silver nanoparticles appears as a new means to reduce the toxicity of silver compounds in the wound care system. Here, following our previous method, AgNPs was synthesized using the culture filtrate of Brevibacillus brevis KN8(2) then characterized by UV-visible spectrophotometry, TEM, SAED, XRD and DLS measurements. The antibacterial activity of AgNPs was evaluated against the most common wound infecting pathogens Pseudomonas aeruginosa and Staphylococcus aureus by well diffusion assay. Further, the wound healing efficacy of biogenic AgNPs was examined in streptozotocin-induced diabetic mice by measuring wound area clo...
DIABETIC DELAYED WOUND HEALING AND THE ROLE OF SILVER NANOPARTICLES
Diabetes mellitus is most common disease of the altered glucose homeostasis. Diabetics have impaired wound healing and impaired formation of coronary collaterals. The abnormal apoptosis or angiogenesis may cause many of the clinical manifestations of diabetes. Silver has been known to have effective bactericidal properties for centuries. Nowadays, silver-based topical dressings have been widely used as a treatment for infections in burns, open wounds, and chronic ulcers. Silver nanoparticles are novel nanosized and highly crystalline antibacterial agent which carries Ag + ions by ionexchanging. This review is an attempt to illustrate the molecular signaling of the apoptosis involved in delayed wound healing and the role of silver nanoparticles in earlier healing.
Antibiotics
Infected cutaneous ulcers from diabetic rats with Candida albicans and Streptococcus aureus were treated with spray formulations containing green silver nanoparticles (GS), chemical silver nanoparticles (CS), or pomegranate peel extract (PS). After wound development and infection, the treatments were performed twice per day for 14 days. The wound healing was analyzed on days 2, 7, and 14 through the determination of CFUs, inflammatory infiltrate, angiogenesis, fibroplasia, myeloperoxidase, and collagen determination. Expressive improvement in wound healing was noted using both silver nanoparticles for 7 days. All the treatments were superior to controls and promoted significant S. aureus reduction after 14 days. CS presented better anti-inflammatory results, and GS and CS the highest number of fibroblasts. Despite the techniques’ limitations, GS and CS demonstrated considerable potential for managing infected wounds, especially considering no early strategies prior to the drugs, suc...
Scientific Reports
To eliminate the microbial infection from an injury site, various modalities have been developed such as dressings and human skin substitutes. However, the high amount of reactive oxygen species, microbial infection, and damaging extracellular matrix remain as the main challenges for the wound healing process. In this study, for the first time, green synthesized silver nanoparticles (AgNPs) using Teucrium polium extract were embedded in poly lactic acid/poly ethylene glycol (PLA/PEG) film to provide absorbable wound dressing, with antioxidant and antibacterial features. The physicochemical analysis demonstrated, production of AgNPs with size approximately 32.2 nm and confirmed the presence of phytoconstituents on their surface. The antibacterial assessments exhibited a concentration-dependent sensitivity of Staphylococcus aureus and Pseudomonas aeruginosa toward biosynthesized AgNPs, which showed a suitable safety profile in human macrophage cells. Furthermore, oxidant scavenging as...