PERBANDINGAN KADAR FLAVONOID PADA EKSTRAK ETANOL DAN EKSTRAK ETIL ASETAT DAUN KERSEN (Muntingia calabura L) DENGAN METODE SPEKTROFOTOMETRI UV-VIS (original) (raw)

2018, Jurnal Analis Farmasi

The leaf and fruit kersen have saponin compounds, flavonoids and tannins. which is nutritious as a drug. Flavonoids are polyphenol compounds that have high antioxidant activity. The purpose of this study was to determine the levels of flavonoids in ethanol extract and ethyl acetate extract and to find out whether there were significant differences between the two with UV-Vis spectrophotometry method. In qualitative tests, ethanol and ethyl acetate extracts were carried out using a color reaction. In quantitative tests using quercetin mother liquor analyzed using UV-Vis spectrophotometer obtained a maximum wavelength of 438 nm. The qualitative results of ethanol extract formed orange and ethyl acetate extract was formed in yellow which indicated the sample contained flavonoids. The results of quantitative analysis of the average level of flavonoids of ethanol extract were 5.12% and ethyl acetate extract was 52.56% with maceration time for 2 days. Statistical results using the ttest obtained t count 234.0661 greater than ttable with a value of freedom 4 with a 99% confidence level of 4.60. The conclusion of this study is that there are significant differences in flavonoid levels of ethanol extract and ethyl acetate extract.

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