In vivo and in vitro studies on Thevetia Species Growing in Egypt I: Isolation, Identification, and Quantification of cardiac glycosides in in vivo and in vitro cultures of immature seeds (original) (raw)

2010

Abstract

In vivo and in vitro extracted cardiac glycosides of immature seeds (IS) cultures of Thevetia neriifolia Jussieu. and T. thevetioides Kunth. were chemically identified. Calli were grown on modified Murashige & Skoog (MS) medium supplemented with 1mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) +3mg/l kinetin (Kin). The content of cardiac glycosides in IS cultures of T. neriifolia and T. thevetioides were monitored by HPLC. Two major compounds were detected and isolated from IS extracts i.e. digitoxigenin and thevetin B. The different structures of the in vivo and in vitro isolated compounds were verified by means of MS and NMR spectral analysis, as well as those compounds were identified and determined using HPLC technique. (Journal of American Science 2010;6(11):390-395). (ISSN: 1545-1003).

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