Cucumeropsis mannii (African White Melon) Seed Oil Mitigates Dysregulation of Redox Homeostasis, Inflammatory Response, and Apoptosis in Testis of Bisphenol A Exposed Male Rats (original) (raw)

2022, Nigerian Journal of Biochemistry and Molecular Biology

Testicular toxicity is a prime threat to male reproductive health. Humans are constantly exposed to Bisphenol A (BPA) via oral route, thus inducing reproductive toxicity. Cucumeropsis mannii seed oil (CMSO) has nutraceuticals with antioxidant potential. This present study investigated the potential of CMSO to mitigate testicular oxidative damages induced by exposure to BPA. Thirty-six (36) male Wistar albino rats, 2-3 months old, were randomly assigned into six (6) study groups; 3 control groups (CG1, CG2, and CG3) and 3 test groups (TG1, TG2, and TG3), respectively. CG1 rats received 1 ml of olive oil. CG2 rats received 100 mg/kg body weight of BPA. CG3 rats received 7.5 ml/Kg body weight of CMSO. TG1 rats were co-administered 100 mg/kg body weight of BPA and 7.5 ml/kg bodyweight of CMSO. TG2 rats were co-administered with 100 mg/kg body weight of BPA and 5 ml/kg body weight of CMSO. TG3 rats were co-administered with 100 mg/kg body weight of BPA and 2.5 ml/kg body weight of CMSO. After the trial, the rats' testes were extracted and taken for biochemical analysis. The result showed that the exposure of rats to BPA significantly (p < 0.05) increased the levels of ROS and MDA but considerably decreased the levels of GSH, CAT, SOD, GPx, IL-1β, IL-6, TNF-α, NF-ĸB, and Caspase-3 activity in the testicular homogenate. However, co-administration of BPA with CMSO significantly (p < 0.05) reversed all the dysregulations. This study reported that CMSO is a potential nutraceutical for mitigation of BPA-induced testicular oxidative damages.