Apparent dissociation constant (K_Dapp) for the WT and mutant MC1 proteins obtained from EMSA experiments (original) (raw)

2014

Abstract

<p>Binding curves (Supplementary <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0088809#pone.0088809.s003" target="_blank">Figure S3</a>) obtained from EMSA experiments were fitted with the equation: Y = [MC1] / ([MC1] + K_Dapp), where Y is the fraction of the bound 26 bp DNA, and [MC1] is the free protein concentration approximated by the total protein concentration in our experimental conditions. Each K_Dapp value is the mean ± standard deviation of three independent experiments.</p

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