A microfabricated silicon platform with 60 microfluidic chips for rapid mass spectrometric analysis (original) (raw)

Many biological processes are controlled by reversible phosphorylation of proteins. Identification of protein phosphorylation sites is crucial for thorough understanding of cell signaling pathways. This study aims at developing tools for phosphoproteomic research. Applicability of an integrated multi-tip photocatalytic micropillar nanoreactor electrospray ionization (µPESI) chip for phosphopeptide analysis has been preliminary studied. Easy and fast switching between individual tips allows rapid mass spectrometry (MS) analysis of large sample series. The titanium dioxide (TiO 2) coating of the chip allows rapid and simple enrichment of phosphopeptides as well as enables photocatalyzed oxidation reactions.