Study of creatine kinase activity in rat brain [Vplyv starnutia a hypoperfúzie na aktivitu kreatínkinázy v mozgu potkanov] (original) (raw)

Abstract

Ischemic stroke results from a transient or permanent reduction in cerebral blood flow. Since nerve cells are exclusively dependent on oxidative phosphorylation, the reduction of substrates, particulary oxygen and glucose, affects all cellular metabolic pathways, especially decrease of ATP production. The present study was focused on the study of creatin kinase activity (CK) - important enzyme for brain energetic metabolism. Activity of this enzyme was study in brain homogenate, mitochondria and synaptosomal fraction in the two groups of Wistar rats (4 months old and 14 months old) using brain hypoperfusion induced by 3-vessel occlusion model without and with reperfusion (15 min of hypoperfusion and 15 min of hypoperfusion following by 24 hrs reperfusion). The significante differences were found in the following fractions: CK activity was significantly increased after hypoperfusion in 4 months old rat brain in homogenate (169.83 ± 12.31 μkat/mg proteins) in comparison with 14 months...

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