Brain ischemia-reperfusion induced alteration in levels of oxidative damage products (original) (raw)

Abstract

Oxidative stress is recognized factor of ischemia reperfusion injury. It shares damage of lipids (LPO) and proteins (PPO). Global 15-min ischemia followed by 2, 24 and 48 hour reperfusion was induced by four vessels occlusion in Wistar rats of both sexes. In forebrain homogenates levels of TBARS and conjugated dienes were analyzed as parameters of LPO. Concentrations of total free sulfhydryl (SH) and carbonyl groups were measured to quantify PPO. Our results indicate that lipid peroxidation and protein oxidation occurs mainly during a period of reperfusion. However, signifi- cant increase in conjugated dienes level can be detected already after 15- min ischemia. Attack of proteins by free radicals lead to modification in structure of proteins seen as decrease of free SH groups and increase in concentration of carbonyls. Ischemia/reperfusion induces formation of lipid peroxidation products as well as protein modifications.

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