Direct coupling of capillary liquid chromatography with conventional high-performance liquid chromatography (original) (raw)

A capillary column liquid chromatograph was combined with a conventional liquid chromatograph and the system was evaluated by using aromatic hydrocarbons as test analytes. A capillary fused-silica column of 0.35 mm I.D. was employed in the former chromatograph. The combination of normal-phase and reversed-phase separations was possible because the first dimension involved separation with the capillary column, which resulted in very small peak volumes. A whole sample band from the former chromatograph could be transferred to the sample loop of the latter. The system was applied to the normal-and reversed-phase separation of aromatic hydrocarbons in a fuel for body warmers (benzine).