Tomato (Lycopersicon esculentum Mill.) juice restored the number of Leydig cells, and the diameter of the seminiferous tubules of mice (Mus musculus) exposed to lead acetate (original) (raw)
Related papers
Tomato ( Lycopersicon esculentum ) prevents lead-induced testicular toxicity
Journal of Human Reproductive Sciences, 2009
The animals were acclimatized over a period of 2 weeks. Preparation of tomato paste Tomato paste (TP) was prepared by grinding tomatoes and heating in a water bath for 45 min at 80 o C. Grouping of animals and treatment The rats were grouped into three groups (Groups A, B, and C, n = 5). Animals in Group A served as the control group and were drinking distilled water. Animals in Groups B and C were drinking 1% Pb (II) acetate (LA). [12] Group C animals were, in addition to drinking LA, treated with 1.5 ml of TP/day. All treatments were for 8 weeks. Animal sacrifi ce and collection of samples Twenty-four hours aft er the last treatment, each animal was sacrifi ced by cervical dislocation and blood samples were collected via heart puncture. Blood sample obtained from each rat was divided into two: One half in a plain bott le and the other half in an ethylenediamminetetraacetic acid
Protective Effects of Wheat Sprout on Testicular Toxicity in Male Rats Exposed to Lead
Reproductive System & Sexual Disorders, 2015
Objective: Negative effects of lead on the male reproductive system and sperm fertility parameters have been shown broadly. In recent years, use of medicinal herbs in reducing heavy metal toxicities has increased worldwide. One of these herbals, wheat sprout, contains high amount of vitamins (especially vitamin E), antioxidants and phytoestrogen compounds. This study investigated the effects of wheat sprout extract (WSE) and vitamin E on testicular oxidative stress in rats exposed to lead acetate. Methods: Thirty-five rats were divided randomly into seven groups: G1 (control group) received 1 ml/kg/day of normal saline, G2 received 20 mg/kg/day of lead acetate, G3 and G4 received 100 mg/kg/day and 200 mg/kg/day of WSE respectively, G5 and G6 received 100 mg/kg/day and 200 mg/kg/day of WSE respectively with 20 mg/kg/day of lead acetate, and G7 received 100 mg/kg/day of vitamin E with 20 mg/kg/day of lead acetate. After 35 days, rats were sacrificed and blood, sperm, liver and testicle tissue samples were collected for histomorphological and histochemical studies. Results: Results showed that count, motility and viability of sperms decreased following the administration of lead acetate (P<0.01). Histomorphological studies showed a significant decrease in tubular differentiation index (TDI), spermiogenesis index (SI), repopulation index (RI), number of Leydig and Sertoli cells, and epithelium height and diameter of seminiferous tubules in groups receiving lead acetate (P<0.05). Conclusion: Summary, results of the current study show that dose dependent WSE significantly prevents testicular toxicity and oxidative stress effects of lead acetate.
Toxicology Letters, 2000
Chlordane, is one of many environmental pollutants that reach human or animal body through food and water consumption, its presence may frequently be associated with other metals such as lead. In this work, the toxicity of chlordane and lead to reproductive tissues in Swiss mice was studied. Oral daily doses of 75 and 275 mg/kg b.w. were administered to male mice, for 35 days, animals were sacrificed at the 2nd, 3rd, 4th, and 5th week. The results show that chloradane had a damaging effect on testicular tissues, the effect was obvious through reduction in diameter of the seminiferous tubules, number of spermatogonia, and primary and secondary spermatocytes and spermatids. The effect was remarkably increased by the presence of lead, which needs more investigation in order to determine whether it's a synergistic or additive effect.
Archives of Biological Sciences, 2013
Lead is an industrial pollutant that may exert specific toxic effects on male mammals. The aim of this study was to investigate further the protective effects of vitamin E on lead acetate (Pb)-induced reproductive toxicities and genotoxic effects on male rats. Sexually mature male Wistar rats (weighing 120-160 g) were given Pb (20 mg/Kg) and vitamin E (600 mg/kg/rat) orally for 20 days. The sperm count, sperm motility, sperm morphology, chromosomal aberrations, FSH, LH and testosterone levels, and histopathological changes in the testes of the rats were investigated after 20 days. Results revealed a statistically significant (p<0.01) increase in the number of abnormal sperm in treated animals. Lead acetate increased the percentage of chromosomal abnormalities. A significant decrease in LH, FSH and testosterone were observed in the treated group compared to the control. Pathological examination of testicular tissues showed degenerative changes of spermatogonia and spermatocytes to advanced degeneration and vacuolation. Lead acetate can be considered to have an environmental genotoxic and cytotoxic effect in the male rat and may contribute to a reduction of fertility. Vitamin E administration could reduce the genotoxic effect of lead in somatic and germ cells.
African Journal of Biomedical Research, 2015
The brain is highly sensitive to lead intoxication. Plant-derived products with antioxidant activity have been useful in reducing lead-induced neurotoxicity. The present study investigated the possible protective effect of tomato pomace powder (TPP) on brain damage induced by lead acetate (LAc) in rats. Thirty rats were divided equally into five groups: control; propylene glycol; TPP (50 mg/kg), LAc (50 mg/kg), and LAc+ TPP. All treatments were administered orally by gavage for 42 days. Rats were euthanized on day 43 of experiment. Behavioural tests, oxidative and blood parameters were done and brain tissue was examined with regard to histological parameters. Results indicated that LAc significantly (p<0.05) induced increased levels of lipid peroxidation and activity of SOD, but reduced GSH level. Similarly LAc caused alteration in the haematological and behavioural parameters, and microscopic anatomy of the cerebellum, dentate gyrus, and Cornu Ammonis3 of rats. These alterations...
The Journal of Basic & Applied Zoology, 2015
Lead is a poison that affects virtually every system in the body. The current study was planned to examine the toxic effects of lead acetate on the histological picture of testes, and the protective roles of DMSA, combined vitamins C and E, and DMSA combined with vitamin C plus vitamin E against the histopathological changes in the testes of albino rats induced by lead acetate. Oral administration of lead acetate caused necrosis of spermatogenic cells in the seminiferous tubules, congestion of interstitial blood vessels, severe interstitial edema and complete necrosis in the seminiferous tubules. Co-administration of DMSA with lead acetate minimized the histopathological changes exhibited by lead acetate in the affected organ compared with lead acetateintoxicated rats. Lead acetate combined with vitamin C plus vitamin E supplemented rats showed mild congestion of the interstitial blood vessels and the seminiferous tubules with its components appeared normal compared to DMSA treated rats. Treatment with DMSA combined with vitamin C plus vitamin E showed more or less normal histological appearance of the testes in lead acetate induced histopathological changes in the affected organ. These results show that DMSA, as a chelating agent for lead, and the combination of vitamins C and E as antioxidants reduced the toxic effects of lead on the histological structure of testes in albino rats but did not provide complete protection. Whereas, the supplementation of DMSA combined with both vitamins C and E provide complete protection against toxicity induced by lead in the testes of albino rats.
IRJPMS, 2022
Background: Solanum aethiopicum (SA), commonly known as garden egg or eggplant can be eaten either fresh or cooked and has a wide acceptance and large history of consumption in West Africa. This study focused on interventions of the hydroethanolic fruit extract of S. aethiopicum (SA) on lead-induced testicular toxicity in adult male Wistar rats. Methods: Twenty-five (25) adult male Wistar rats were randomly divided into five groups (1-5) of five (5) rats per group. Group 1 served as the control and was given 10 ml/kg/day of distilled water only, Group 2 received 10mg/kg body weight (B.W) of lead acetate only, Group 3 received 100 mg/kg B.W SA and 10mg/kg B.W of lead acetate (Pb), Group 4 received 200 mg/kg B.W of SA and 10mg/kg B.W of lead acetate while Group 5 received 300 mg/kg B.W of SA and 10mg/kg B.W of lead acetate. The administration of the extract was done using gastric gavage once a day, for thirty (30) consecutive days. Semen analysis (to assess sperm parameters such as sperm count, sperm motility, sperm viability and normal morphology), Testicular weight and testicular histology were assessed. Testicular oxidative stress markers, such as Malondialdehyde, Superoxide dismutase, Catalase and reduced Glutathione were also assessed. Results: Lead acetate caused a significant decrease in the sperm parameters and testicular antioxidant markers. It also caused a significant distortion of the histology of the testes and an insignificant decrease in testicular weight. The hydroethanolic fruit extract of SA however was found to cause an increase in testicular weight, enhanced sperm parameters, increase in the antioxidant parameters and a decrease in Malondialdehyde in all the lead-treated groups. There was also a marked improvement in the testicular histology after SA administration. Conclusion: It was concluded that S. aethiopicum fruit offers protection against free radical mediated oxidative stress of rats with lead induced testicular toxicity.
The Effect of Antioxidant Gallic acid on the Testes of Lead Acetate Induced Wistar Rat
Toxicol. Environ. Health., 2018
Background: Lead is considered one of the major causes of infertility among occupational workers in manufacturing companies such as electric storage batteries, glass and ceramic wares. This study was carried out to confirm the effect of Gallic acid as an antioxidant on Lead acetate treated male albino rats. Methods: Twenty albino rats weighing between 140 g and 200 g were divided equally into four groups. Group A served as the control, group B was treated with 60 mg/kg body weight of Lead acetate, group C received 50 mg/kg body weight of Gallic acid and group D received both Lead acetate and Gallic acid for 14 days. Results: The result showed slight increase in the body weights and testes of group B and D compared to the control group and there was a slight decrease in group C. Rats in group B showed a significant increase in MDA (3224.63±89.38 unit/mg protein) but a slight increase was observed in the level of MDA (1537.70± 53.92 unit/mg protein) in group D. The level of Lactate Dehydrogenase (LDH) in group B was 338.75±18.87 nmol min mL, which was the highest among the groups and significant. Conclusion: Lead acetate is toxic to the gonad, it causes degeneration and reduction in spermatogenic activity in seminiferous tubules. However, the effect of Lead on the male reproductive organ can be reverse significantly by an antioxidant (gallic acid) due to its ability to scavenge for oxidative stress caused by Lead.
Effect of Ascorbic Acid on Reproductive Function of Male Rats Exposed to Lead Acetate
Background: The anti-oxidant ascorbic acid (AA) is known as a chelating agent in treatment of lead (Pb) toxicity, and has been reported to protect the cells from oxidative stress. Objective(s): This work aims to study the efficiency of ascorbic acid on semen quality, sex hormone, antioxidant parameters and testis histology in rats treated with lead acetate. Methods: A total of 50 male rats were divided into five equal groups; control group (received tap water only), Pb group (received 0.2% lead acetate/kg, BW) and the other three groups (received 500, 1000 and 1500 mg/kg BW AA along with 0.2% lead acetate/kg BW), respectively. Doses (as solutions) were orally administered every day for 8 weeks. Motility, validity, abnormal and dead sperm were assessed. Testosterone, luteinizing (LH) and follicle-stimulating (FSH) hormones were measured. Antioxidant activity [glutathione (GSH), thiobarbituric acid reactive substances (TBARS), total antioxidant capacity (TAC) and the level of nitric oxide (NO)] were determined. Histopathological examination was done for testis. Results: The results showed that Pb caused a significant increase in number of abnormal and dead sperms in Pb group (43.0%, 67.2%) comparing to the control group (6.0%, 18.6%) respectively. Motility and validity of the sperm were significantly decreased in Pb group (16.0%, 32.8%) comparing to the control group (84.2%, 81.4%) respectively. Pb caused a significant increase in FSH (1.99 mIU/L) and LH (1.2 mIU/L) and a decrease in Testosterone hormones (0.86 nmol/L) comparing to the control group (0.64 mIU/L, 1.2 mIU/L, 5.24 nmol/L) respectively. On the other hand, AA caused a significant decrease in numbers of abnormal and dead sperms than in Pb group. AA also caused an increase in motility and viability of the sperms at all levels. Testosterone hormone showed a marked increased with AA and the best effect was found with the high level (1500 mg /kg BW). For antioxidant activity it was found that Pb caused a significant increase in NO and TBARS levels comparing to the control group, while it decreased significantly GSH and TAC levels. The significant effect for AA was found with the high level (1500 mg) on NO (28.5 µmol/ml) and GSH (4.9 µmol/ml). Also, it was found that AA significantly affected TBARS and TAC at all levels. Histopathological examination showed the presence of AA reduced the harmful effect of lead acetate on testis. Conclusion: High daily intake of AA from rich sources or from supplementation can protect reproductive system of male rats from lead toxicity.
Folia Medica Indonesiana
Lead is the free radicals and heavy metals of major pollutants in the environment. Lead is toxic and cumulative. Red dragon fruit (Hylocereus polyrhizus) peel extract is a natural antioxidant. Red dragon fruit peel extract can be used to stabilize free radicals by supplementing electron deficiencies and inhibiting chain reactions. This study aimed to analyze therapy effect of red dragon fruit peel extract to increase the number of Sertoli cells on BALB/c mice (Mus musculus) exposed to lead acetate. Fourty mice were divided into 5 groups (each group consisted of 8 mice). K- group was the control group without lead acetate and dragon fruit peel extract administration, K + group was given 100 mg/KgBW lead acetate orally on the 1st day until day 14th.. P1, P2, and P3 group were continued with red dragon extract orally on the 15th day until 39th day. P1 with dose 250 mg/KgBW, P2 with dose 500 mg/KgBW, and P3 with dose 1000 mg/KgBW. The result showed significant difference (P<0.05) in ...