Development of a high-capacity cell migration/chemotaxis assay for monocytes (original) (raw)

Cancer Research, 2008

Abstract

1544 Cell migration is crucial for embryonic development, the inflammatory immune response, wound repair, and tumor invasion and metastasis. Monocytes circulate in the blood stream for about one to three days and then typically migrate into tissues throughout the body. In immune responses, monocyte migration is critical for providing immunological defense and eliminating infectious agents. Circulating monocytes have the capacity to differentiate into a variety of phagocytes, including macrophages, dendritic cells, osteoclasts, microglia in the central nervous system, and Kupffer cells in the liver. To facilltate the assessment of the migratory capacity of monocytes against various chemotactic stimuli and the effect of inhibitory compounds on monocyte migration, we developed a convenient high-capacity migration/chemotaxis assay for monocytes. The Monocyte Cell Migration Assay Kit is based on the widely accepted Boyden Chamber principle and in a 96-well format. The presented assay pr...

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