Genotypic variations in the inhibitory potentials of four combined botanicals on mycelia growth of Macrophomina phaseolina of cowpea [Vigna unguiculata (L) Walp.] (original) (raw)

The ethanol extracts of Ficus asperifolia, Mormordica charantia, Anacardium occidentals and Psidium guajava were evaluated sole and in treatment combinations at 25, 50 and 75mg ml concentration levels against the mycelial growth of Macrophomina phaseolina of Cowpea. The pathogen was cultured on plates containing botanicals amended Potato Dextrose Agar (PDA) in three replicates while only ethanol treated PDA tested plates served the control experiment. The radial growths were recorded at 4, 6 and 8 day after inoculation. Data obtained were analysed using the SAS software program version 9.2. The extract of Mormordica charantia was the most effective in the botanical treatments alone. The most significant inhibition of Macrophomina phaseolina were observed from the combined treatments of Ficus asperifolia, Mormordica charantia and Anacardium occidentals (3.11 cm), followed by Mormordica charantia and Psidium guajava (3.29 cm), then combination of four extracts; Ficus asperifolia, Morm...