Genetic assessment of Mangifera indica Linn. (Mango) from selected locations in Oyo State, Nigeria (original) (raw)

Characterization of Mangifera indica cultivars in Thailand based on macroscopic, microscopic, and genetic characters

Journal of Advanced Pharmaceutical Technology & Research, 2016

Thai mango cultivars are classified into six groups plus one miscellaneous group according to germplasm database for mango. Characterization is important for conservation and the development of Thai mango cultivars. This study investigated macroscopic, microscopic leaf characteristics, and genetic relationship among 17 cultivars selected from six groups of mango in Thailand. Selected mango samples were obtained from three different locations in Thailand (n = 57). They were observed for their leaf and fruit macroscopic characteristics. Leaf measurement for the stomatal number, veinlet termination number, and palisade ratio was evaluated under a microscope attached with digital camera. DNA fingerprint was performed using CTAB extraction of DNA and inter-simple sequence repeat (ISSR) amplification. Forty-five primers were screened; then, seven primers that amplified the reproducible band patterns were selected to amplified and generate dendrogram by Unweighted Pair-Group Method with Arithmetic Average. These selected 17 Thai mango cultivars had individually macroscopic characteristics based on fruits and leaves. For microscopic characteristics, the stomatal number, veinlet termination number, and palisade ratio were slightly differentiable. For genetic identification, 78 bands of 190-2660 bps were amplified, of which 82.05% were polymorphic. The genetic relationship among these cultivars was demonstrated and categorized into two main clusters. It was shown that ISSR markers could be useful for Thai mango cultivar identification.

DNA POLYMORPHISM AND GENETIC DIVERSITY OF MANGO (Mangifera sp.) GERMPLASM IN TROPICAL ISLAND

International Journal of Biosciences and Biotechnology

Creation of new superior varieties of mango through the empowerment of local genetic resources requires information about the potential properties of mango germplasm, including DNA polymorphism and its genetic diversity. This research aimed to obtain basic data on DNA polymorphism, genetic relationship, genetic similarity level, and molecular accession of Bali’s unique local mango. Sampling was conducted in four regencies in the Province of Bali. DNA preparation, PCR, and microsatellite analysis were carried out at the Laboratory of Genetics and Plant Breeding, Faculty of Agriculture, Gadjah Mada University. It found 44accessions of mangoes in Bali. The test results with 10 pairs of SSR markers showed that all primers produced polymorphic loci. There were 825 amplified DNA bands. Primer of AY31 produced the highest number of loci, which were 14 loci, while AY21 produced the fewest loci, i.e., 3 loci. Forty-four mango accessions showed a genetic similarity coefficient of 0.27 to 0.97...

Morphological characterization of mango (Mangifera indica L.) cultivars from south-west Nigeria

Ife Journal of Science, 2019

Mango (Mangifera indica L.) is an economically important tropical fruit consumed all over the world. So far, little attention has been paid towards the documentation and characterization of its cultivars. Occupying a unique position among edible fruit crops in Nigeria, information on the exact number of cultivars has not been recorded, leading to a lack of accurately named germplasms and cultivars. To gain phenomenal insight on cultivars' diversity, a morphological technique employing both cluster and principal component analysis were adopted. Morphological characters of seven cultivars collected from National Horticultural Research Institute (NIHORT), Ibadan Nigeria, which includes: leaf length and width, leaf apex, petiole length, fruit length and width, fruit colour and shape were assessed. The results of principal component analysis based on similarity matrix revealed a correlation between leaf length and petiole length, fruit length, width and shape whereas fruit colour exhibited no correlation with any of the other parameters. Cluster and dendrogram analysis based on farthest neighbour, mean character difference and constrained clustering strategy, revealed that mango cultivars are distinctively divided into two groups based on their fruit features: Cluster A comprises Julie, Edward, Palmer, and Kent whereas cluster B comprises Saigon, Madoe and Lipen. This study provides a solid baseline for the further characterization of the different mango cultivars in Nigeria, and will be useful for germplasm management and crop breeding.

Phylogenetic relationship among Mango (Mangifera indica L.) Landraces of Saurashtra based on DNA fingerprinting

… of Horticulture and …, 2011

Twenty mango cultivars (Mangifera indica Linn) collected from Gir region of Saurashtra were examined by ISSR markers. Of the 50 primers screened, 21 primers gave reproducible, polymorphic DNA amplification patterns, and were selected to construct a DNA fingerprinting map to distinguish the genotypes of mango. According to the banding patterns obtained with 21 selected primers, all cultivars tested in this study except Jamadar and Kesar were distinguished from each other and showed ample genetic diversity, indicating that ISSR-PCR was an effective method for cultivar identification of mangoes. Based on 125 selected bands, all Gir mango landraces tested were clustered into a three big groups with ‛Kaju' and ‛Khodi' in first group, Dudh Pendo, Sopari, Jamadar, Kesar and Ashadhiya in second group, while the third cluster was composed of Agargato, Amir Pasand, Pethal, Gajariyo, Chhappaniyo, Alphanso, Neelum, Jamrukhiyo, Kavasji Patel, Giriraj, Amrutiyo, Dasheri and Deshi based on UPGMA analysis, indicating that some Gir landraces had a close relationship with each other, while some were drastically dissimilar from other landraces.

Morphological and molecular analysis of indigenous Myanmar mango (Mangifera indica L.) landraces around Kyaukse district

Journal of Plant Biotechnology

There is vast genetic diversity of Myanmar Mangoes. This study mainly focused on indigenous thirteen different mango landraces cultivated in central area of Myanmar, Kyauk-se District and their fruit characteristics by 18 descriptors together with genetic relationship among them by 12 SSR markers. Based on the morpho-physical characters, a wide variation among accessions was found. Genetic characterization of thirteen mango genotypes resulted in the detection of 302 scorable polymorphic bands with an average of 4.33 alleles per locus and an average polymorphism information content (PIC) of 0.7. All the genotypes were grouped into two major clusters by UPGMA cluster analysis and a genetic similarity was observed in a range of 61~85%. This study may somehow contribute insights into the identification of regional mango diversity in Myanmar and would be useful for future mango breeding program.

Genetic Analysis of Mango (Mangifera indica. L) Genotypes for Year-round Flowering and Yield Characters

Madras Agricultural Journal, 2022

Mango (Mangifera indica. L) is characterized by a high level of genetic variation in seedling population. Estimation of genetic advance is required for the expected genotypic progress of a particular character. Keeping this in view, genetic advances of year-round flowering mango (Mangifera indica. L) genotypes was carried out during 2019-2021 in farmer’s field at Annur, Coimbatore. High Genotypic Coefficient of Variation (GCV) was found in fruit yield per tree was 30.63 per cent. The low GCV values were found for the traits viz., panicle length (6.61 %), days taken for flowering to fruit set (1.75%), days taken for flowering to fruit maturation (1.76 %), percentage of hermaphrodite flowers (5.38 %), sex ratio (5.18%). Whereas high values of heritability estimates were obtained for the traits viz., fruit yield per tree (99.43 %), number of panicles/tree (98.91 %), number of fruits per tree (97.74 %), panicle per sq.m of canopy (97.40 %). The high heritability along with high genetic ...

Genetic Diversity In Mango (Mangifera Indica L.) Through Multivariate Analysis

Bangladesh Journal of Agricultural Research, 2013

The genetic divergence was assessed in 60 mango genotypes through D2- statistics and principal component analysis. The genotypes under study were grouped into eight clusters and the diversity was influenced by the morphological characters, not by the geographical distribution of the genotypes. The clustering pattern revealed that the genotypes collected from the same region did not fall in the single cluster. The maximum inter cluster distance was noticed between cluster II and cluster VIII, and the lowest between clusters VII and cluster VIII. From the cluster means, cluster I was high yielding and ranked first in terms of number of secondary branches per inflorescence, percent fruit set per inflorescence, and yield per plant. Cluster VIII had only one genotype which produced the highest percentage of flowering shoots, % perfect flowers, number of fruits per plant, and %TSS. The genotypes of cluster VII produced the biggest sized fruits. The first nine characters of the principal c...

Study of Different Variants of Cv. Langra (Mangifera indica L.) Based on Morphological and Yield Characters

Current Journal of Applied Science and Technology, 2020

Study of morphological and yield characters of different genotypes of mango are easy and convenient methods to identify promising and desired genotype during breeding programme. So, keeping in view, the trial was conducted under the experimental area of BAU, Sabour with objective of morphological and yields characterization of the variants of cv. Langra during the year 2012-13. The variants of cv. Langra significantly varied in respect of tree height(6.20 m to 11.40 m), trunk girth (95.00 cm to 280.00 cm), tree spread (East-West: 8.45 m to 17.40 m, North-South: 9.15 m to 17.05 m), duration of flowering (16.00 days to 20.00 days), fruit maturity (101.00 days to 123.00 days), fruit size(length: 8.58 to 13.66 cm, breadth: 6.39 to 10.87 cm), fruit weight (182.20 g to 843.90 g), fruit volume (171.40 ml to 699.40 ml), numbers of fruit per tree (32.50 to 1127.00 fruits) and fruit yield per tree (10.95 to 358.83 kg). These results shows the significant variations in genetic level of diffe...

Morphological, Molecular Characterization and Breeding for Biotic and Abiotic Stress in Mango (Mangifera Indica L.)

Acta Horticulturae, 2015

Mango (Mangifera indica L.) is the most important fruit crop in India having socioeconomic significance. Morphological characterization involves the study of development of form, structure with attempts being made to interpret these on the basis of similarity and origin. Owing to the high heterozygosity and seedling propagation, nomenclature ambiguity is one of the main impediments in classification of mango. Under the morphological characterization the widely used keys are the ones developed by 'Bioversity International' which has 54 characteristics. Morphological characterization has assisted in the classification of cultivars but ambiguity in nomenclature persists due to its limitation by way of minor differences between the characteristics. Molecular markers are used to measure diversity at DNA level and are not much influenced by environmental conditions like morphological markers. Classification of cultivars and screening of cultivars for biotic and abiotic stress would help in selecting the parents for the hybridization program and development of recombinants resistant to various biotic and abiotic stresses. Breeding attempts for abiotic stress made in mango have centered on the rootstock improvement in few places where soil salinity is a problem. Screening of polyembryonic cultivars has shown the cultivars 'Olour' and 'Bappakkai' to be tolerant compared to others.