Pollution-Induced Community Tolerance of Soil Microbial Communities Caused by the Antibiotic Sulfachloropyridazine (original) (raw)
Related papers
Soil Biology and Biochemistry, 2005
A method for the detection of the effects of antibiotics on soil microbial communities was optimised in the present study. We investigated the influence of measurement time and nutrient status on the pollution-induced community tolerance (PICT), using the sulfonamide sulfachloropyridazine (SCP) as model compound. The tolerance development in soil microcosms that were exposed to SCP under different conditions was compared with the background tolerance in SCP-unexposed microcosms. The tolerance of bacterial extracts from the soil microcosms was determined in Biolog w multiwell plates as the SCP sensitivity of a range of physiological processes. The background tolerance was not affected by soil nutrient amendments, but an influence of the inoculum density in the microtiter plates was observed. Still, the variance of the background tolerance was low, which is in favour of the usage of community tolerance measurements for a selective detection of a toxicant impact. In line with the assumptions of the PICT concept, soil amendment with SCP led to an increase in community tolerance. This tolerance development was enhanced upon additional soil amendment with fresh pig slurry, and less by alfalfa meal addition. Tolerance increases were observed after a soil exposure to SCP of only 7 days, possibly because nutrient input facilitates the fast adaptation of the soil microbial community. However, a further increase in exposure time led to variable changes in the observed tolerance. Prolonged tolerance detection in the microtiter plates (11 days) enabled a clearer differentiation between different soil treatments, as it better resolved the EC 50 values of processes with a high tolerance to bacteriostatic antimicrobial compounds. For the detection of antibiotic effects on soil microbial communities, it is therefore recommended to use nutrient amendments (possibly fresh pig slurry), to standardize the soil exposure time, and to extend the period of Biolog plate measurement beyond 7 days. q
International Journal of Environmental Research and Public Health
The toxicity exerted by the antibiotic sulfadiazine on the growth of soil bacterial communities was studied in two agricultural soils for a period of 100 days. In the short-term (2 days of incubation), the effect of sulfadiazine on bacterial growth was low (no inhibition or inhibition <32% for a dose of 2000 mg·kg−1). However, sulfadiazine toxicity increased with time, achieving values of 40% inhibition, affecting bacterial growth in both soils after 100 days of incubation. These results, which were here observed for the first time for any antibiotic in soil samples, suggest that long-term experiments would be required for performing an adequate antibiotics risk assessment, as short-term experiments may underestimate toxicity effects.
2014
Veterinary antibiotics such as sulfadiazine (SDZ) are applied with manure to agricultural soil. Antimicrobial effects of SDZ on soil microbial community structures and functions were reported for homogenized bulk soils. In contrast, field soil is structured. The resulting microhabitats are often hot spots that account for most of the microbial activity and contain strains of different antibiotic sensitivity or resilience. We therefore hypothesize that effects of SDZ are different in diverse soil microhabitats. We combined the results of laboratory and field experiments that evaluated the fate of SDZ and the response of the microbial community in rhizosphere, earthworm burrow, and soil macroaggregate microhabitats. Microbial communities were characterized by phenotypic phospholipid fatty acid (PLFA) and genotypic 16S rRNA gene patterns (DGGE) and other methods. Data was evaluated by principle component analyses followed by two-way ANOVA with post-hoc tests. Extractable SDZ concentrations in rhizosphere soil were not clearly different and varied by a factor 0.7-1.2 from those in bulk soil. In contrast to bulk soil, the extractable SDZ content was two-fold larger in earthworm burrows, which are characterized by a more hydrophobic organic matter along the burrow surface. Also, extractable SDZ was larger by up to factor 2.6 in the macroaggregate surface soil. The rhizosphere effect clearly increased the microbial biomass. Nonetheless, in the 10 mg SDZ kg −1 treatment, the biomass deceased by about 20% to the level of uncontaminated bulk soil. SDZ contamination lowered the total PLFA concentrations by 14% in the rhizosphere and 3% in bulk soil of the field experiment. Structural shifts represented by Pseudomonas DGGE data were larger in SDZ-contaminated earthworm burrows compared to bulk soils. In the laboratory experiment, a functional shift was indicated by a four-fold reduced acid phosphatase activity in SDZ-contaminated burrows compared to bulk soil. Structural and functional shifts after SDZ contamination were larger by a factor of 2.5 in the soil macroaggregate surface versus interior, but this relation reversed over the long-term under field conditions. Overall, the combined effects of soil microhabitat, microbial community composition, and exposure to SDZ influenced the microbial susceptibility towards antibiotics under laboratory and field conditions.
Soil Biology and Biochemistry, 2006
Veterinary medicines enter agricultural soils by the use of animal excrements as fertilizers. To study their impact on soil bacterial communities, microcosms containing orthic luvisol soil were spiked with the antimicrobial agents sulfadiazine (SDZ) and chlorotetracycline (CTC) at three different concentrations (1, 10, 50 mg kg À1 soil) and incubated for 48 days at 20 1C. The impact on the microbial respiratory activity was measured continuously in a respirometer (Sapromat). Changes in bacterial community structure were visualized by means of PCR-denaturing gradient gel electrophoresis (DGGE) of 16S rDNA derived from soil samples after 1, 7, 11 and 48 days. Additionally, growth inhibitory effects of SDZ and CTC on bacteria previously isolated from the same soil were tested in agar diffusion tests. In microcosms with soil and antibiotics only, no effects could be observed, either on respiratory activity or on bacterial population structure. Therefore, further incubations were conducted in the presence of an additional assimilable carbon source (5 g glucose kg À1 soil). In the presence of glucose, SDZ affected soil respiration as well as the bacterial community structure: Additional bands appeared and some bands already visible at the beginning of incubations increased in intensity. A clear relationship between SDZ concentrations and changes in DGGE patterns became visible. During 48 days of incubation, changes in DGGE patterns were minimal in microcosms with 50 mg SDZ kg À1 soil indicating an inhibition of strains, which were capable of growing on glucose in the presence of lower SDZ concentrations. Only a few soil bacterial isolates (5 out of 47 strains tested) were weakly inhibited by SDZ in agar diffusion disk tests. Contrastingly, CTC inhibited growth of 12 soil bacterial isolates significantly in disk tests, but no effects on soil respiration and bacterial community structure could be observed. In the presence of the soil matrix the growth inhibitory potential of CTC decreased due to adsorption or complexation. This was confirmed in growth inhibition experiments with soil suspensions and time-dependent sampling. r
Analysis, fate and effects of the antibiotic sulfadiazine in soil ecosystems
TrAC Trends in Analytical Chemistry, 2009
This review summarizes current knowledge about the interplay between fate and effects of the antibiotic sulfadiazine in soil ecosystems. In applying manure from antibiotic-treated animals to arable soils, sulfadiazine can reach the environment, but fate and transformation processes and the consequences for soil microorganisms and soil functions have not been studied adequately. Since antibiotics are explicitly designed to affect microorganisms, they are likely to affect ''non-target'' microbes in the soil ecosystem. Recent papers provide new insights into the disappearance dynamics of sulfadiazine, its effects on distinct microbial communities and the development of antibiotic resistance. ª
Journal of Hazardous Materials, 2021
Extracellular polymeric substances (EPS) play a dominant role in protective biofilms. However, studies exploring the underlying protective mechanism of EPS have mainly focused on activated sludge, whereas their positive roles in protecting soil microbes from environmental stress have not been elucidated. In this study, we revealed the response of soil bacterial communities to various dosages of sulfadiazine (SDZ) present in manure, with a special emphasis on the role of EPS. Sequencing analysis showed that the bacterial community demonstrated stronger symbiotic relationships and weaker competitive interaction patterns to cope with disturbances induced by SDZ. EPS was mainly composed of tyrosine-like and tryptophan-like substances, and moreover, carboxyl, hydroxyl and ether groups were the main functional groups. An adaptation mechanism, namely the enhanced secretion of tryptophan-like substances, could help alleviate the SDZ stress effectively in the biofilms occurring in soil that experienced long-term manure application. Furthermore, the existence of EPS weakened the accumulation of antibiotic resistance genes (ARGs) in soil. Our results for the first time systematically uncover the joint action of biofilm tolerance and ARGs in resisting SDZ stress, which enhances understanding of the protective role of EPS and the underlying mechanisms governing biofilm functions in soil environments.
Applied microbiology and biotechnology, 2014
Sulfadiazine (SDZ) is an antibiotic frequently administered to livestock, and it alters microbial communities when entering soils with animal manure, but understanding the interactions of these effects to the prevailing climatic regime has eluded researchers. A climatic factor that strongly controls microbial activity is soil moisture. Here, we hypothesized that the effects of SDZ on soil microbial communities will be modulated depending on the soil moisture conditions. To test this hypothesis, we performed a 49-day fully controlled climate chamber pot experiments with soil grown with Dactylis glomerata (L.). Manure-amended pots without or with SDZ contamination were incubated under a dynamic moisture regime (DMR) with repeated drying and rewetting changes of >20 % maximum water holding capacity (WHCmax) in comparison to a control moisture regime (CMR) at an average soil moisture of 38 % WHCmax. We then monitored changes in SDZ concentration as well as in the phenotypic phospholi...
1ère conférence internationale d’Ecotoxicologie Microbienne, EcotoxicoMic, 2017
Arslan (Arslan et al. Dysbiosis in plant-endophyte partnership: repeated short exposures of sulfamethoxazole and trimethoprim at micro-concentrations can disturb the microbial community in soft rush, Juncus effusus) SESSION TROPHIC INTERACTIONS CLOSING Cheloni (Cheloni et al. Morphological plasticity in Chlamydomonas reinhardtii: an adaptive response to micropollutant stress) Crouzet (Nazaret) (Crouzet et al. Monitoring the impact of multi-stress contamination on an environmental opportunistic pathogen, Stenotrophomonas maltophilia: application to antibiotics and metals) Neury-Ormanni (Neury-Ormanni et al. Changes in the interactions between periphytic microalgae and micromeiofauna induced by environmental exposure to diuron and/or imidacloprid) Grosjean (Grosjean et al. Genome-wide analyses of rare earth elements responsive genes in microbial model organisms) Proia (Proia et al. Biofilm phosphorus uptake capacity responds to chemical and environmental stressors in river ecosystems) Pascoal (Pascoal et al. Impacts of silver nanoparticles on aquatic detritus-based food webs) Coffee Break Coffee Break Spina (Spina et al. Evaluation of the ecotoxicologycal impact of municipal wastewaters on wildlife: a case study in Tuscany) Coffee Break Chaumet (Chaumet et al. Transfer and distribution of diuron in biofilms and joint toxic effects) Rossi (Rossi et al. Interactive effects of pesticides and nutrients on microbial communities responsible of litter decomposition in streams) Gallois (Gallois et al. Insights into uranium tolerance of Microbacterium oleivorans A9 by proteogenomic analyses)
Effects of sulfadiazine-contaminated fresh and stored manure on a soil microbial community
European Journal of Soil Biology, 2011
The response of soil microorganisms to manure contaminated by veterinary antibiotics is not well understood. Therefore, a 57-d incubation experiment was performed to investigate effects of sulfadiazine (SDZ) contaminated manure on soil microorganisms. Manure was either obtained directly from medicated pigs or subsequently stored for six month. We hypothesized that SDZ-contaminated manure changes functions and structural composition of soil microorganisms and that manure storage reduces antibiotic effects. Rates of potential nitrification and N mineralization as well as PLFA (phospholipid fatty acid) patterns were determined. Addition of uncontaminated manure to soil affected N cycle processes and the microbial community structure, and effects differed between fresh and stored manure. The rates of potential nitrification, ammonification and N mineralization and ratios of bacteria/fungi and gramnegative/gram-positive bacteria derived from PLFA were lower in soil treated with stored compared to fresh, uncontaminated manure. SDZ-contaminated manure affected N cycle processes with slight differences due to manure storage, whereas PLFA tot and the derived bacteria/fungi ratio were only reduced by stored, contaminated manure. SDZ reduced rates of nitrification and N mineralization, while ammonification increased. Effects of SDZ on soil microorganisms were significant although the bioavailable SDZ fraction (CaCl 2-extractable) rapidly declined. We conclude that conventional manure storage is unsuited to reduce risks from sulfonamide antibiotics in the soil environment.