Sperm quality after swim up and density gradient centrifugation sperm preparation with supplementation of alpha lipoic acid (ALA): A preliminary study (original) (raw)
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An Update of Sperm Preparation : A Review of Supplementation Substances to Improve Sperm Quality
Biomedical and Pharmacology Journal, 2018
One etiology of the unsuccesful intra uterine insemination (IUI) is sperm preparation. There are some limitations of sperm preparation utilization which may decrease fertilization rates leading to IUI failure. Nevertheless, there are some substances which suggested could improve the outcome of sperm preparation. This review aimed to summarize the substances that could improve the sperm quality, thus increasing success rate of IUI, as an update of sperm preparation. Several researches which have developed agreed that the supplementation of antioxidant, hormone and drugs could improve the quality of sperm. The addition of dithiothreitol and superoxide dismutase during centrifugation could prevent some harmful effects such as membrane plasma disruption caused by lipid peroxidation process. The supplementation of myoinositol before and after capacitation increased total and progressive motile sperm significantly. Astaxanthin decreased the generation of ROS in sperm. Furthermore, LH could increased intracellular Ca 2+ as a second messenger of signal transduction pathways during sperm capacitation, whereas prolactine prolonged human sperm motility and prevent caspase activation. In addition, the supplementation of pentoxifylline could enhance the motility of post-thaw sperm and its longevity in vitro.
Effect of In-Vitro Alpha Lipoic Acid Addition on Spermatozoa Motility in Sperm Preparation Process
Folia Medica Indonesiana
Infertility is a problem for husband and wife, in the last 20 years the number of infertile couples has tended to increase by around 6.5 million pairs. The infertile couple can use the intrauterine insemination method to obtain offspring if a conventional method approach cannot be performed. Insemination requires a sperm preparation stage in which there are centrifugation and resuspension procedures that tend to produce excess reactive oxygen species (ROS). Excessive ROS will damage the motility of the spermatozoa. This study aims to prove the addition of alpha lipoic acid (ALA) as an antioxidant in the process of sperm preparation to improve and maintain better sperm motility. This research is a laboratory study with an experimental research design. The sample consisted of 10 infertile men who visited the Andrology section of the Sayyidah Jakarta Mother and Child Hospital (RSIA), where each ejaculate from the patient would be divided into 3 groups namely (k1) fresh semen as a contr...
Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya, 2021
Freeze-drying technique is an alternative method on sperm preservation. However, this process can results in DNA damage. Adding antioxidants before freezing can reduce the detrimental effects of ROS on spermatozoa. Alpha lipoic acid (ALA) is one of the potent antioxidant that can be used to protect the sperm DNA. This study was aimed to determine the effect of ALA supplementation before freeze-drying on the DNA fragmentation index (DFI) of human spermatozoa. Nine semen samples were collected and evaluated for DFI (O1) then divided into 3 groups; freeze-drying without ALA (O2), freeze-drying with ALA 1,25 mg (O3) and freeze-drying with ALA 2,5 mg (O4).All three groups were stored at 4oC for 1 week. Sperm DFI were evaluated before and after freeze-drying. The result of this study showed a significant increase in sperm DFI in all three groups after freeze-drying (p<0,05) when compared to DFI before freeze-drying (14,67 ±3,295). However there was no significant difference between O2 ...
Efficacy of combination of multinutrients in reduction of sperm DNA fragmentation
International journal of health sciences
High Sperm DNA fragmentation can cause decreased fertility & implantation rate or can cause Recurrent implantation failure and if fetus born then can lead to Genetic abnormality in the fetus such as Down Syndrome, Edward syndrome or Patau’s syndrome. The objective of the study is to combine the peripheral data of the patients to evaluate the safety and efficacy of Combination tablet in reduction of Sperm DNA fragmentation index. The result shows that the factors affecting the DFI .The Institutional Ethics Committee approved the study procedure (Ref No: SRMC/RP/4505).
Effects of antioxidants on human sperm preparationtechniques
Int J Androl, 1994
The effect of two different sperm preparation techniques, Percoll gradient centrifugation and swim-up from a washed pellet were tested on the functional competence of the selected spermatozoa. Percoll gradient centrifugation brought about an improvement in sperm motility parameters such as curvilinear velocity and straight-line velocity, an increase in the rates of hyperactivation and the acrosome reaction and an increase in the percentage of motile spermatozoa after 24 h of incubation compared to the centrifugation, swim-up procedure. The effects of antioxidants such as dithiothreitol (DTT) o r reduced glutathione (GSH), and reactive oxygen species-scavenging enzymes such as catalase or superoxide dismutase (SOD) during the stage of centrifugation before the swim-up procedure were also studied. Though all of these agents prevented the fall in sperm motility after 24 h incubation, only DTT and SOD improved the rates of both hyperactivation and the acrosome reaction. GSH also improved the acrosome reaction, whereas catalase was without significant effect on the rates of hyperactivation or the acrosome reaction. These results indicate that Percoll gradient centrifugation selects spermatozoa with better functional competence than does centrifugation swim-up. The damage caused by the centrifugation can be prevented by the addition of antioxidants, suggesting that the differences noted with the Percoll gradient method was due to better protection against peroxidative damage due to the centrifugation of unselected spermatozoa.' However, the use of DTT is limited by virtue of the fact that this sulphydryl reducing agent leads to destabilization of the sperm chromatin. In contrast, GSH and SOD could have therapeutic potential.
Effect of antioxidant therapy on the sperm DNA integrity improvement; a longitudinal cohort study
International Journal of Reproductive BioMedicine
Background: The effect of antioxidant therapy on sperm DNA fragmentation index (DFI)and achieving natural pregnancy were under debate. Very few studies have showedthe rate of pregnancy rate after the antioxidant therapy due to ethical and technicallimitations.Objective: The aim of this cohort study was to determine the improvement rate ofsperm DFI and natural pregnancy rate after the antioxidant therapy in infertile men.Materials and Methods: 1645 infertile men were subjected for this study from May2015 to December 2017. The Spermogram and sperm DFI were assessed using WorldHealth Organization (WHO) 2010-based protocols and sperm chromatin structure assay(SCSA), respectively, in sperm samples before and after antioxidant therapy.Results: The total sperm DFI improvement rate was 38.9% in the total population.Sperm DFI improvement had close correlation with total motility (r= 0.731, p= 0.001)and progressive motility improvement (r= 0.885, p= 0.001); 16.8% of individuals whocompleted a...
ICSI in cases of sperm DNA damage: beneficial effect of oral antioxidant treatment
Human Reproduction, 2005
BACKGROUND: Most studies examining the use of ICSI for cases of elevated sperm DNA fragmentation report poor pregnancy and implantation rates. ICSI with testicular sperm samples has recently been suggested for these cases. Here we test a less invasive approach based on oral antioxidant treatment prior to ICSI with ejaculated spermatozoa. METHODS: Thirty-eight men with an elevated ($15%) percentage of DNA-fragmented spermatozoa in the ejaculate were treated with antioxidants (1 g vitamin C and 1 g vitamin E daily) for 2 months after one failed ICSI attempt. In 29 (76%) of these cases this treatment led to a decrease in the percentage of DNA-fragmented spermatozoa, and a second ICSI attempt was performed. Outcomes of the two attempts were compared. RESULTS: No differences in fertilization and cleavage rates or in embryo morphology were found between the ICSI attempts performed before and after the antioxidant treatment. However, a marked improvement of clinical pregnancy (48.2% versus 6.9%) and implantation (19.6% versus 2.2%) rates was observed after the antioxidant treatment as compared with the pretreatment ICSI outcomes. CONCLUSIONS: Oral antioxidant treatment appears to improve ICSI outcomes in those patiens with sperm DNA damage, in whom this treatment reduces the percentage of damaged spermatozoa.
A study of the antioxidant effect of alpha lipoic acids on sperm quality
Clinics, 2008
OBJECTIVE: Assisted reproductive techniques are useful in helping infertile couples achieve successful conception. Initial studies have shown that sperm cryopreservation, one step in assisted reproduction, causes a dramatic reduction in sperm quality. This has been attributed to, among other things, free radical activities. The aim of the present study was to minimize this oxidative attack by adding an antioxidant into the sperm microenvironment. Alpha lipoic acids were selected for this purpose for their efficient free radical scavenging properties and solubility in lipid and aqueous phases. METHODS: For this investigation, semen from six Boer bucks was pooled. Seminal analysis of the baseline prior to incubation of samples with different concentrations of Alpha lipoic acids (0.00625, 0.0125, 0.025, 0.05, 0.1 mmol/ml) was performed, and postseminal analysis was conducted after a one-hour incubation. The comet assay was used to observe the effect of Alpha lipoic acids on sperm DNA integrity. Statistical analysis using an unpaired t-test with a significance level of p<0.05 was then performed. RESULTS: Our results indicate that the sperm motility rate was improved after incubation with Alpha lipoic acids at a concentration of 0.02 mmol/ml. This concentration was also capable of reducing DNA damage. CONCLUSION: In conclusion, Alpha lipoic acids renders cryoprotection to sperm, thereby improving sperm quality.
Reduction of the Incidence of Sperm DNA Fragmentation by Oral Antioxidant Treatment
Journal of Andrology, 2005
Sperm DNA fragmentation is known to compromise male fertility. Previous findings have suggested the implication of oxidative stress in the etiology of this pathological condition. The present study was conducted to find out if the pathologically increased incidence of DNA fragmentation in ejaculated spermatozoa can be reduced by oral treatment with two antioxidants, vitamins C and E. Sixty-four men with unexplained infertility and an elevated (Ն15%) percentage of DNA-fragmented spermatozoa in the ejaculate were randomized between an antioxidant treatment (1 g vitamin C and 1 g vitamin E daily for 2 months) group and a placebo group. Sperm DNA fragmentation was evaluated by terminal deoxyribonucleotidyl transferase-mediated dUTP nick-end labeling assay before and after treatment. No differences in basic sperm parameters were found between the antioxidant treatment and the placebo group before or after treatment. However, the percentage of DNA-fragmented spermatozoa was markedly reduced (P Ͻ .001) in the antioxidant treatment group after the treatment (9.1 Ϯ 7.2) as compared with the pretreatment values (22.1 Ϯ 7.7). No difference in the pretreatment and posttreatment incidence of sperm DNA fragmentation was observed in the placebo group. These data show that sperm DNA damage can be efficiently treated with oral antioxidants administered during a relatively short time period.
P-037. Effect of antioxidant supplementation in vitro and in vivo on human sperm DNA integrity
Human Reproduction, 1997
means. Sperm morphology slides from 1988 were also randomly selected, mixed with a proportional number of slides from 1996 and re-evaluated. Results: Relatively uniform patterns were obtained between the three population groups with regard to age, sperm concentration and sperm morphology (percentage normal). Both age and sperm concentration showed an increase over the study period, whereas normal sperm morphology showed a strong decline over the same period, with correlation coefficients of-0.8505,-0.9521 and-0.9463 (P < 0.0001) for Blacks, Coloureds and Whites respectively. The mean ± SD percentage of morphologically normal spermatozoa, as calculated in 1996, was 2.9% lower than the original mean for 1988 (9.5 ± 5.5 and 12.4 ± 8.0% for 1996 and 1988 respectively). Conclusion: No significant decline was observed over the study period in the number of days of sexual abstinence or in the following semen parameters: motility, forward progression and volume. The most likely factors that may have caused the decline in the percentage of morphologically normal forms over the years are the changes in evaluation criteria and possible changes in environmental and socioeconomic factors.