The early inflammatory response to bites of Leishmania major infected Phlebotomus duboscqi sand flies in naïve and pre-exposed mice. (94.15) (original) (raw)

The Journal of Immunology

Abstract

Pre-exposure to uninfected sand fly bites (USFB) protects against Leishmania major infection. Little is known about the initial immune response responsible for this protection. During blood feeding, the insect sand fly inoculates Leishmania parasites into the skin alongside saliva. Here, we explore the early inflammatory response in the skin of pre-exposed (PE) mice challenged with L. major-infected P. duboscqi flies. The relative expression of cytokines and chemokines by skin cells were investigated 2- 48h post-infection using a macroarray (Oligo GEArray®). There is a rapid and strong early expression of inflammatory genes in PE compared to naive mice following challenge. Two to twelve hours post-infection, naïve mice showed an increased expression of MCP-1, MCP-3 and eotaxin that attract macrophages and granulocytes while PE mice upregulated TCA-3, BRAK and I-TAC related to migration of NK cells, macrophages, granulocytes and activated T cells. Characterization of leukocytes by F...

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