Isolation and screening of microalgae from agro-industrial wastewater (POME) for biomass and biodiesel sources (original) (raw)

Desalination and Water Treatment, 2016

Abstract

ABSTRACT Indigenous microalgae were isolated from agro-industrial wastewater in Johor, Malaysia and were screened for the greatest goal of mass cultivation in IPASA, UTM Skudai in order to determine their potential as biomass and biodiesel sources. Bold’s Basal Medium nutrient media recipes were used to isolate the largest number of colonies from each field sample. These nutrient instructions were altered in order to optimize the isolation and growth dynamics of particular colonies. The isolates were classified based on the morphological appearance of the culture and the microscopic cellular appearance of the isolated colonies. Isolates comprised several common green microalgae and cyanobacteria, in which one of the ideal candidates was nominated for further investigation. Lipid content was determined for particular strains that illustrated relatively quick growth. The results revealed that Chlamydomonas incerta achieved the highest lipid content of 42.6% of biomass after 2 days, as well as the highest lipid productivity of 0.197mg/L.d-1 when the initial COD concentration was 500 mg/L. C. incerta that showed the high growth rate, resistance to invasion, and contained adequate amount of lipid was examined for its potential as a sustainable biomass and biodiesel feedstock.

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