Analgesic and Anti-Inflammatory Effects of Hydroalcoholic Extract of Salvia multicaulis on Male Rats (original) (raw)
Related papers
2012
Ethnopharmacological relevance: Salvia officinalis L. has been used as a traditional herbal medicine for gastric disturbances and inflammatory processes. This study investigated the toxicological, antinociceptive and anti-inflammatory effects of the hydroalcoholic extract (HE) from leaves of Salvia officinalis and its isolated compounds in mice. Materials and methods: Mice were treated with HE before the induction of nociceptive response by chemical agents (acetic-acid, formalin, glutamate, capsaicin and cinnamaldehyde). Total leukocytes and plasma extravasation induced by acetic acid and paw oedema induced by glutamate, capsaicin and cinnamaldehyde were also measured. The antinociceptive effect of carnosol and ursolic acid/oleanolic acid were evaluated on formalin and cinnamaldehyde models. Results: In the acute toxicity test the value of estimated LD50 for HE was 44.7579 g/kg. Oral administration of HE (10, 30 and 100 mg/kg) inhibited the number of writhings, total leukocytes and plasma extravasation induced by acetic acid. In the formalin test, HE reduced both neurogenic and inflammatory phases, effect that was affected by naloxone. The glutamate-, capsaicin-and cinnamaldehyde-induced nociception and paw oedema were reduced by HE at doses that did not affect the locomotor activity of mice in the open field test. Carnosol (10 mg/kg) and ursolic acid/oleanolic acid (30 mg/kg) inhibited the inflammatory phase of formalin and the nociception and mechanical allodynia induced by cinnamaldehyde. Conclusions: These results demonstrate that HE presents significant anti-inflammatory and also antinociceptive effects on chemical behavioral models of nociception that involves an opioid mechanism. In addition, carnosol and ursolic acid/oleanolic acid contained in this plant appears to contribute for the antinociceptive property of the extract, possibly through a modulatory influence on TRPA1-receptors. However, further studies regarding the precise site and the mechanism of action of HE and carnosol and ursolic acid/oleanolic acid merited exploring further.
Anti-inflammatory effects of different extracts from three Salvia species
Turkish Journal of Biology
Salvia L. species have been used for the treatment of various infl ammatory ailments in traditional medicine. In order to evaluate this ethnobotanical information, water, methanol, n-butanol, acetone, and chloroform extracts from 3 Salvia species (S. fruticosa, S. verticillata, and S. trichoclada) were screened for their anti-infl ammatory activity using in vivo experimental models in rats. For this purpose a carrageenan-induced infl ammatory paw edema model was used. All extracts demonstrated anti-infl ammatory activities; however, n-butanol extract of Salvia fruticosa (syn. S. triloba), which is known as Turkish sage, was found to be the most active. It can be expected that the active fl avonoids, phenolic acids, and terpenoids may be responsible for the anti-infl ammatory activity of these plants.
In vivo analgesic activity of Salvia wiedemannii Boiss. used in Turkish Folk Medicine
Planta Medica, 2011
The aerial part of Salvia wiedemannii Boiss. (Lamiaceae) has been used for treatment of peptic ulcers and relieving pain in Turkish folk medicine. To evaluate the analgesic effect of S. wiedemannii, tail flick and acetic acid-induced writhing tests were used in mice. The chloroform extract (500 mg/kg, i.p.) obtained from S. wiedemannii showed significant analgesic activity on tail flick assay, while water, ethanol and butanol extracts of the plant had no activity on the same test. Chloroform extract (500 mg/kg, i.p.) also inhibited number of writhings induced by acetic acid. Chloroform extract provided analgesic effects similar to morphine. Its effect was quick and durable. This in vivo study demonstrates that S. wiedemannii has strong analgesic effect as the public believed.
Anti-inflammatory activity of Salvia officinalis L
Journal of Medicinal Plants Research, 2012
This study evaluate the effects of sage hydroalcoholic extract (SE) and sage essential oil (SO) on the inflammatory response using an experimental model of acute inflammation and a leukocyte migration assay. In the carrageenan-induced pleurisy test, SE did not reduce the exudate volume and leukocyte migration to the pleura, but SE exerted a topical anti-inflammatory effect by significantly inhibiting croton oil-induced ear edema. All SO doses tested significantly inhibited leukocyte chemotaxis induced by casein and reduced the number of rolling, adhesion, and leukocytes migration to spermatic fascia after inflammatory stimulus. Our data demonstrated that SO has anti-inflammatory activity.
Analgesic Activity of Salvia wiedemannii Boiss. Used in Turkish Folk Medicine
acgpubs.org
The aerial part of Salvia wiedemannii Boiss. (Lamiaceae) has been used for treatment of peptic ulcers and relieving pain in Turkish folk medicine. To evaluate the analgesic effect of S. wiedemannii, tail flick and acetic acid-induced writhing tests were used in mice. The chloroform extract (500 mg/kg, i.p.) obtained from S. wiedemannii showed significant analgesic activity on tail flick assay, while water, ethanol and butanol extracts of the plant had no activity on the same test. Chloroform extract (500 mg/kg, i.p.) also inhibited number of writhings induced by acetic acid. Chloroform extract provided analgesic effects similar to morphine. Its effect was quick and durable. This in vivo study demonstrates that S. wiedemannii has strong analgesic effect as the public believed.
In Vivo Anti-inflammatory and Antinociceptive Activities of Salvia nilotica and Rosa abyssinica
Ethiopian Pharmaceutical Journal
In vivo anti-inflammatory and antinociceptive activity tests were carried out on the 80% methanol extracts and soxhlet fractions of Rosa abyssinica Lindley (Rosaceae) and Salvia nilotica Juss. Exjecr (Lamiaceae) using carrageenan-induced paw oedema and formalin test, respectively. It was observed that the 80% methanol extracts of both plants could significantly lower carrageenan-induced paw oedema at doses of 200 mg/kg, p.o. Among the solvent fractions, the acetone, chloroform and methanol fractions of S. nilotica exhibited anti-inflammatory activity, the strongest being that of the acetone fraction. On the other hand, only the acetone fraction of R. abyssinica was found to be active. The anti-inflammatory activities of the extracts and the fractions were comparable with that of the known anti-inflammatory drug, indomethacin at the tested concentrations. The results of the formalin test revealed that the acetone fractions of R. abyssinica and S. nilotica significantly inhibit formal...
Some effects of Salvia aegyptiaca L. on the central nervous system in mice
Journal of Ethnopharmacology, 2002
Salvia aegyptiaca L. is used for treating various unrelated conditions that include nervous disorders, dizziness, trembling, diarrhoea and piles. This work examines some effects of the crude acetone and methanol extracts of the plant given at single oral doses of 0.25, 0.5, 1 or 2 g/kg, on the central nervous system (CNS) in mice. The extracts were also tested for anti-inflammatory and antipyretic actions. Several models of nociception have been used to examine the analgesic effect of the extract. In treated mice, the extracts caused dose-related inhibition of acetic acid-induced abdominal constriction, and significantly reduced formalin-induced pain. Treatment with the extracts at doses of 0.5 and 1 g/kg significantly increased the reaction time in the hot-plate test. In treated mice both extracts caused significant and dose-related impairment of the sensorimotor control and motor activity. Treatment with both extracts did not significantly affect the rectal temperature of normothermic mice. The methanol extract (0.5 and 1.0 g/kg) did not affect the rectal temperature of hyperthermic mice, but the acetone extract was effective in significantly reducing the rectal temperature of hyperthermic mice, 0.5 and 1 h after administration of the extract at doses of 0.25 Á/2 g/kg. It is concluded that the crude methanol and acetone extracts of S. aegyptiaca have CNS depressant properties, manifested as antinociception and sedation. Both extracts have some anti-inflammatory and antipyretic actions. On the whole, the acetone extract appeared to be slightly more effective than the methanol extract in this regard. #
In this paper, three decoctions of different Danshen medication forms (crude SM pieces, SM formula granule, liquored SM pieces) were compared to explore their comparative analgesic and anti-inflammatory effect. In three mice models, the hot plate test and the writhing test were used to analyze their analgesic effect, and the ear edema test was carried out to assess their anti-inflammatory effect. Compared with the corresponding model control group, the results suggested that the three different SM decoctions all had definite effect in prolonging t he latency to licking a hind paw or jumping, reducing the writhing rate within 30 min and narrowing the deviation between the weight of ears. Furthermore, liquored SM pieces and SM formula granule had better analgesic and anti-inflammatory effect than crude SM pieces and no obvious differences appeared between liquored SM pieces and SM formula granule.
Spasmolytic, Anti-Inflammatory, and Antioxidant Activity of Salvia Gesneriflora Lindley
African Journal of Traditional, Complementary and Alternative Medicines
Background: Salvia gesneriflora Lindley is employed in traditional Mexican medicine for the treatment of several diseases. Materials and Methods: The chromatographic profile of three extracts (SgH, SgD and SgM) of this plant allowed the identification of 11 components in SgH, the presence of rosmarinic (1), chlorogenic (2) and caffeic (3) acids and quercetin glucoside (4) in SgM and of ursolic acid (5) in SgD. The spasmolytic (electrically induced contractions of guinea-pig ileum), anti-inflammatory (edema in mouse ear) and antioxidant potential (DPPH, ABTS and FRAP assays) of the extracts were evaluated. Results: SgM showed the highest percentage of relaxation (80.67 ± 1.633%) with no significant difference (p
Effects of Salvia officinalis L. extract on experimental acute inflammation
Revista medico-chirurgicală̆ a Societă̆ţ̜ii de Medici ş̧i Naturaliş̧ti din Iaş̧i
The aim of this study was to investigate the effects of Salvia officinalis L. tincture on acute inflammation induced with oil of turpentine. Oil of turpentine (i.m. 0.6 ml/100 g b.w.) was injected into male Wistar rats right hind paw. Salviae folium tincture and diclofenac as positive control (30 mg/100 g b.w.) were administrated i.p. The effects were evaluated by measuring total leukocyte count and differential leukocyte count expressed as a percentage, a test of in vitro phagocytosis and evaluation of nitric oxide synthesis by measuring the metabolites: nitrites and nitrates. Salvia officinalis tincture significantly reduced the total leukocyte and monocytes percentages and the activation of circulating phagocytes. NO synthesis had a slight decrease. Salvia officinalis tincture had a smaller inhibitory effect than diclofenac. Salvia officinalis tincture had antiinflammatory effects by reducing marrow acute phase response and NO synthesis.