Additional file 1: Figure S1. of An improved pre-clinical patient-derived liquid xenograft mouse model for acute myeloid leukemia (original) (raw)

2017

Abstract

Immune profile of BM mononuclear cells from AML patients. a Mononuclear cells isolated from AML patients were immunolabeled with human CD45, CD34, CD38, CD33, and CD117 and analyzed using flow cytometry. Gating using CD45 and CD34 showed three subsets indicating of (i) CD45hiCD34− non-blast cells, (ii) CD45loCD34− blast cells, and (iii) CD45loCD34+ blast cells. Expression of CD38, CD33, and CD117 for each subset was shown. Frequency of CD33+CD117+ relative to total human CD45+ cells in each subset was shown. b Level of primary engraftment from poor responders. Newborn NSG pups were injected intrahepatically with 8.7 × 104–7.9 × 105 cells after sublethal irradiation. Level of engraftment in peripheral blood was determined at specified weeks post-engraftment and in spleen and BM at endpoint using event number of human CD45+ cells divided by the sum of human CD45+ cells and mouse CD45.1+ cells. Data are presented as mean % human CD45+ cells relative to total CD45+ cells ± SEM. (TIFF 32...

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