Examination of 2-methacryloyloxyethyl phosphorylcholine polymer coated acrylic resin denture base material: surface characteristics and Candida albicans adhesion (original) (raw)
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2021
Purpose: To evaluate surface roughness and Candida albicans adhesion of polyamide resin denture base coated with various concentrations of MPC polymer, 2-methacryloyloxyethyl phosphorylcholine. Materials and Methods: A total of eighty polyamide disks were prepared and divided into four groups (n = 20/group). Surfaces of specimens in three groups were treated with MPC in various concentrations (0.25 mol/L; 0.50 mol/L and 0.75 mol/L), whereas uncoated specimens were used as control. Surface roughness (SR) was evaluated using a profilometer. Incubation of the specimens with C. albicans was done overnight, and the count of adhered cells was estimated. Statistically, the following tests were done: analysis of variance, Pearson correlation coefficient test, post hoc Tukey test. Results: Upon PMPC coating, surface roughness values were decreased, with a significant difference between the groups. There was significant decrease in C. albicans count with the increase in the MPC polymer concen...
Materials, 2021
Candida albicans (C. albicans) biofilm is a common etiological factor in denture stomatitis. The purpose of this study was to investigate the effects of incorporating 2-methacryloyloxyethyl phosphorylcholine (MPC) as a protein repellent into a new high-impact denture acrylic (HIPA) resin on the surface roughness, solution pH, and C. albicans biofilm adhesion to the denture base. The new acrylic denture resin base was formulated by mixing MPC into HIPA resin at mass fractions of 1.5%, 3%, and 4.5%. Surface roughness was measured using a Mitutoyo surface roughness tester. C. albicans biofilm growth and viability were assessed via colony forming unit counts. The pH of the biofilm growth medium was measured using a digital pH meter. Adding MPC to the HIPA resin at percentages of 1.5% and 3% increased the roughness values significantly (p < 0.05), while adding 4.5% MPC resulted in no difference in roughness values to that of the control group (p > 0.05). All experimental groups dem...
Biomedical and Pharmacology Journal, 2018
The aim of this study was to evaluate the effect of modified heat cured polymethyl methacrylate denture base (PMMA) or varnish coating of the denture base on candida albicans adhesion and porosity. First experimental group was prepared by addition of 15% phosphoric acid 2-hydroxyethyl methacrylate ester with polymethyl methacrylate monomer. While second experimental group prepared by varnish layer coating after material processing, these groups were compared with the control one. Three groups of vertex acrylic resin used as control, mPMMA and varnish coated group (PPH Cerkamed Wojciech Pawlowski, Poland). The inverted light microscope was used to measure the candida albicans adherence after one week and one month of processing. Two-way ANOVA, LSD test, T- test and Dunnett T3 were performed on data. Modified Acrylic resin showed highly significant reduction in candida albicans adhesion and porosity, also for varnish coating group showed highly significant reduction in candida albican...
This study evaluated the surface roughness and Candida albicans adherence on denture base acrylic resins and silicone-based resilient liners with different surface finishes. Four commercial denture base acrylic resins (three heat polymerized and one room temperature polymerized) and five silicone-based liner materials (two heat polymerized and three room temperature polymerized) (10×10×2 mm) were tested in this study. The materials were processed against glass or plaster or finished with a tungsten carbide bur. Surface roughness measurements were made using a profilometer with an optical scanner probe. All specimens were ultrasonically cleaned in water for 15 s, autoclave sterilized, and contaminated with C. albicans solution for adherence assay evaluation. The materials processed against the glass surface showed significantly lower surface roughness values (0.11±0.1-1.66±1.1 μm) than those of the materials processed against the dental plaster (2.61±0.2-6.12±2.8 μm) or roughening with a bur (1.48±0.2-7.05± 1.2 μm; p<0.05, one-or two-way analysis of variance). Also, the materials processed against the glass surface showed lower C. albicans adhesion (mean ranks 120.36) than those of the materials processed against the dental plaster (mean ranks 139.77) or roughening with a bur (mean ranks 143.06), but the differences were not statistically significant (p>0.05, Kruskal-Wallis and Mann-Whitney). In all types of surface finishes, C. albicans adhesion on denture base acrylics was significantly less (mean ranks 90.18-90.40) than those of silicone liners (mean ranks 119.38-205.18; p<0.01, Kruskal-Wallis).
Polymers
Denture stomatitis is a multifactorial pathological condition of the oral mucosa that affects up to 72% of denture wearers. It is commonly seen on the palatal mucosa and characterized by erythema on the oral mucosa that are in contact with the denture surface. The aim of this study was to incorporate 2-methacryloyloxyethyl phosphorylcholine (MPC) and dimethylaminohexadecyl methacrylate (DMAHDM) into a high impact polymethylmethacrylate heat-cured denture base acrylic resin as a potential treatment for denture stomatitis. We used a comparative study design to examine the effect of incorporating MPC as a protein repellent agent and DMAHDM as an antifungal agent to prevent the adherence of Candida albicans to the denture base material. The dual incorporation of MPC and DMAHDM reduced C. albicans biofilm colony-forming unit by two orders of magnitude when compared to the control group devoid of the bioactive agents. Although the addition of MPC and DMAHDM alone or in combination signifi...
Polymers, 2022
Denture stomatitis is a common oral infection caused by Candid albicans. It occurs under removable dentures due to several causative and contributing factors. If not treated, it can lead to fatal systemic candida infections. Different materials and techniques have been used to treat denture stomatitis; however, no single treatment has succeeded. The purpose of this study was to review novel techniques for incorporating antimicrobial and protein repellent agents into denture acrylic resin materials and their effect on the adhesion of Candida albicans to denture base acrylic resin materials to prevent and/or treat denture stomatitis. A systematic review was conducted in which an electronic search was undertaken using different databases and relevant keywords. The literature search revealed numerous studies describing different antifungal materials incorporated into different denture acrylic resin materials. The investigated materials showed significant antimicrobial activity with slig...
Al-Rafidain Dental Journal, 2013
Aims: This study aims to measure the effect of three different types of polishing materials (pumice, pumice with soap solution and universal polisher paste) on the surface roughness and the growth of Candida Alicans (C.A) on two types of denture base materials (heat cured acrylic and flexible thermoplastic resin). Materials and Methods: Ninety (90) samples with dimensions (3cm length, 3cm width & 1cm thickness) were prepared & divided into two main groups (45 samples in each) according to the type of base material, heat cured & flexible. Each group was subdivided into three subgroups (15 samples in each) according to the type of polishing materials used in this study: Group1: Polished with pumice (control group), Group 2: Polished with (pumice with soap solution), Group 3: Polished with universal polisher paste. Roughness was measured to all samples by Profilometer Tester. After that the samples were incubated in media containing C.A. for 72 hr. at 300 C to count and compare the value of C.A. growth & adhesion.Results: the statistical analysis of roughness test between the heat cured & flexible showed a highly significant differences between samples polished with pumice, t-test=10.594, p<0.01, and significant differences for both groups polished by (pumice + soap solution) & (polishing paste), t-test= 4.651 & 3.173 with p<0.05 respectively. And for both types (Heat cured & Flexible), samples polished with pumice have the lowest number of viable cells count of C.A. (54, 67 respectively) Conclusions: The heat cured acrylic resin samples had a smoother surface than flexible samples. (Pumice) produced smoother surface than (universal polishing paste) and (pumice +soap solution), therefore, C.A. adhesion were higher than samples polished with pumice.
Journal of dentistry (Shiraz, Iran), 2017
Candida species are believed to play an important role in initiation and progression of denture stomatitis. The type of the denture material also influences the adhesion of candida and development of stomatitis. The aim of this study was comparing the adherence of candida albicans to the conventional and injection molding acrylic denture base materials. Twenty injection molding and 20 conventional pressure pack acrylic discs (10×10×2 mm) were prepared according to their manufacturer's instructions. Immediately before the study, samples were placed in sterile water for 3 days to remove residual monomers. The samples were then sterilized using an ultraviolet light unit for 10 minutes. 1×10(8) Cfu/ml suspension of candida albicans ATCC-10231 was prepared from 48 h cultured organism on sabouraud dextrose agar plates incubated at 37oC. 100 μL of this suspension was placed on the surface of each disk. After being incubated at 37oC for 1 hour, the samples were washed with normal saline...
2020
Background: PTFE is an anti-adherent and aesthetic material. The aim of this study was to evaluate, in vitro, the influence of PTFE coating on the biofilm formation between Polytetrafluoroethylene (PTFE) coated denture resin surfaces and uncoated denture resin surfaces. Materials and Methods: Two groups were tested [Group 1: control, pure polymethylmethacrylate(PMMA); Group 2: modified PMMA with PTFE coating. Thirty resin specimens for each group were polymerized, and four experimental subgroups for each surface type were devised, consisting of 2 and 4 days of incubation in C. albicans suspension. After incubation one set for sample was taken for colony counting whereas other set was used for crystal violet staining and UV spectrophotometer assay. Results: At day 2, the PTFE coated resin had statistically significantly lower levels of Candida than the uncoated group. The PTFE coated resin had statistically significantly lower levels of Candida accumulation at days 2 and 4 compared t...
Candida albicans adhesion on reinforced polymethylmethacrylate denture resin: effect of fibre architecture and exposure to saliva Background and objectives: Fiber-reinforced composites (FRC) are used to reinforce the prosthetic and restorative appliances. This may result in the exposure of the FRCs which may affect the adherence of microorganisms. This study evaluated the adhesion of Candida albicans to a denture base resin (N = 48), reinforced with E-glass FRCs with different architectures [unidirectional (n = 16) and woven (n = 16)], when exposed to either saliva or distilled water. Materials and methods: Specimens without FRC reinforcement served as control (n = 16). After fiber surfaces were exposed, half of the specimens (n = 8/per group) were treated with saliva, the other half was stored in distilled water prior to C. albicans adhesion. The adhered microorganisms were counted under an optical microscope and the data were analyzed. Results: When exposed to distilled water or saliva, specimens with unidirectional (5403.4 cells/cm 2 and 5013.4 cells/cm 2 , respectively) (p = 0.202), woven fibers (4053.5 cells/cm 2 and 3726.6 cells/cm 2 , respectively) (p = 0.283) and specimens without fibers (2250 cells/cm 2 and 2006.8 cells/cm 2 , respectively) (p = 0.423) showed C. albicans adhesion, being not significant. In general, all the specimens exposed to saliva showed 3582.2 cells/cm 2 C. albicans adhesion, while specimens exposed to distilled water showed 3902 cells/cm 2 C. albicans adhesion, yet being not significant (p = 0.436). Regarding fiber type, C. albicans adhesion was significantly affected by the exposed FRC architecture with more favorable results for woven fibers (p < 0.001). Conclusion: Polymethylmethacrylate without FRCs collected less C. albicans. The presence of saliva seems to reduce the adhesion.