Knockout Rats via Embryo Microinjection of Zinc-Finger Nucleases (original) (raw)
This study explores the use of engineered zinc-finger nucleases (ZFNs) for generating knockout rats by inducing targeted mutations in specific genes. By injecting ZFN-encoding DNA or mRNA into rat embryos, the researchers achieved high rates of successful mutations in regards to the green fluorescent protein (GFP) and two endogenous rat genes, Immunoglobulin M (IgM) and Rab38. The findings demonstrate that ZFNs can effectively disrupt target genes without off-target effects, showcasing their potential for genetic modification in rat models.