A STUDY ON METHICILLIN RESISTANT COAGULASE NEGATIVE STAPHYLOCOCCI ISOLATED FROM MEDICAL INTENSIVE CARE UNIT AT A TERTIARY CARE CENTRE-INDIA (original) (raw)
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https://www.ijhsr.org/IJHSR\_Vol.10\_Issue.6\_June2020/IJHSR\_Abstract.052.html, 2020
Introduction: CONS are part of the normal skin flora increasingly recognized as significant nosocomial pathogens and often causes different infections associated with implanted devices, joint prosthesis and different indwelling devices. They are very difficult to treat as they are more resistant to commonly used antimicrobial agents than others. Because of that, present study was conducted with following aims and objectives. Aims and Objectives: To study antibiotic susceptibility profile of isolated species of CONS to various classes of antimicrobials using Kirby Bauer disc diffusion method followed by detection of methicillin resistance and inducible clindamycin resistance among the isolated species of CONS. Materials and Methods: 170 CONS strains isolated from clinically significant samples were identified by different conventional methods and antibiotic resistance pattern was detected by Kirby-Bauer Disk Diffusion method by using different antibiotic discs. Methicillin resistance and Inducible and constitutive clindamycin resistance (D test) was detected according to CLSI guidelines. Results: Among 170 CONS isolates, predominant isolated species were S. epidermidis (42.35%), S. haemolyticus (27.06%) and showed higher resistance to different antibiotics. 105(61.76%) isolates showed erythromycin resistance, out of which, 26 (24.76%) isolates were iMLS B. 113 (66.47%) isolates were MRCONS. Conclusion: Present study showed high prevalence of MRCONS, resistant to widely used antimicrobial agents. Hence, it is necessary to have regular surveillance of MRCONS which will be useful for selecting an appropriate antibiotic.
The aim of this study was to evaluate methicillin resistance detection methods currently used when studying coagulasenegative staphylococci (CoNS). The resistance to oxacillin of 142 strains from seven species of CoNS isolated from the Intensive Care Unit environments was tested. The methods used were: disc diffusion test with cefoxitin (FOX 30 ) and oxacillin (OX 1 ), oxacillin agar screen test with 6 mg/l of oxacillin (MHOXA), latex test for PBP2a (LA) and detection of mecA via PCR. One hundred and one isolates were methicillin-resistant in at least one of methods used, but only 74 were mecA-positive. Of the 68 mecA-negative strains: two were positive by OX 1 , the LA and MHOXA methods; three by the LA and MHOXA; and 22 only by OX 1 test. Most of these strains were from the novobiocin-resistant CoNS group. The results obtained for all tested strains using FOX 30 showed complete concordance with the presence of the mecA gene.
Journal of Applied Microbiology, 2001
Aims: To achieve reliable detection of methicillin resistance in clinical isolates of coagulasenegative staphylococci. Methods and Results: Strains (105) were evaluated by normatized antimicrobial susceptibility methods, and for the presence of the methicillin resistance-determining mecA gene, using the polymerase chain reaction. Correlation between phenotypic and genotypic methods was obtained in 87á6% of the samples. Six strains, classi®ed as methicillin-susceptible by phenotypic assays, revealed the presence of the mecA gene, indicating that methicillin resistance expression was probably repressed. Another seven isolates failed to show mecA ampli®cation after displaying methicillin resistance in phenotypic evaluations. The susceptibility of the methicillin-resistant isolates to other antimicrobial agents was variable. Conclusions: Genotypic determination of the mecA gene proved to be the most reliable method for detection of methicillin resistance. Signi®cance and Impact of the Study: Correct assessment of methicillin resistance, such as that attained through genotyping, is essential for de®ning therapeutic strategies, particularly when treating severely compromised patients.
Panacea Journal of Medical Sciences, 2023
Abstract Background: Coagulase negative staphylococci have been recognized as one of the important cause of hospital acquired infections in recent past. It has become difficult to manage these infections due to emergence of multidrug resistance to them. The aim of this study was to determine antibiotic sensitivity trend of all Coagulase negative Staphylococci isolates using modified Kirby- Bauer disk diffusion technique. Materials and Methods: To assess the antibiotic sensitivity for coagulase negative staphylococci, Kirby-Bauer disc diffusion method on Muller-Hinton agar was used. The study findings were interpreted on the basis of CLSI standards. All coagulase negative staphylococci isolates were given a predetermined panel of antibiotics. Methicillin-resistant coagulase negative staphylococci were identified using Cefoxitin disk. Results: The sensitivity to Linezolid, Vancomycin and Rifampicin was found in all 500 isolates of coagulase negative staphylococci in our study. However, they were resistant to Penicillin G (65%), Cefoxitin (56%), Ciprofloxacin (57%), Levofloxacin (32%), Gentamicin (21%), Erythromycin (67%), Clindamycin (60%), Cotrimoxazole (51%) Tetracycline (9%). Conclusion: As CoNS have emerged as an important agent of hospital acquired infections, hence there is need of identification, speciation and resistance pattern of isolates for better management of patients. Keywords: Coagulase Negative Staphylococci, Methicillinresistant coagulase negative Staphylococci, Antibiotic susceptibility
2008
Objective: To identify the Methicillin Resistant Coagulase Negative Staphylococci (MRCONS) and Methicillin Resistant Staphylococcus aureus (MRSA) from clinical specimens and their antimicrobial susceptibility patterns. Methodology: All strains were recovered from clinical specimens according to 0.5 McFarland standard inoculated on Muller Hinton agar. Antibacterial susceptibility was tested by disks diffusion method. Results: Of 346 Staphylococci, 250 (72.25%) were identified as methicillin resistance. From these strains, ninety three (37.2%) were MRSA and 157 (62.8%) identified as MRCONS. The highest rate of MRCONS identified in S. haemolyticus and the lowest rate of MRCONS belonged to S. intermedius and S. schleiferi. The susceptibility pattern of MRSA and MRCONS against other antimicrobial agents revealed that the lowest resistance was for vancomycin.
IP innovative publication pvt. ltd, 2019
Introduction: Coagulase negative staphylococci (CoNS) which were formerly regarded as contaminants of clinical samples, these undoubtedly need more recognition as their pathogenic potential is being increasingly understood and can cause serious human infections. They are causing problems to clinicians because of their drug resistance. Susceptibility testing should be done an isolate considered to be a cause of infection because of the resistance of these organisms to a wide spectrum of antimicrobial agents. Materials and Methods: Total One hundred strains of CoNS were isolated from 1486 clinical Samples processed (blood – 320, urine – 518, exudates – 522 and body fluids-126.Out of 100 CoNS were isolated from blood (35), urine (33), exudates (29), and body fluids (3) samples collected from both outpatients and inpatients of our Hospital. The organisms were identified and speciation was done by standard biochemical reactions. Antibiotic susceptibility testing was done by Kirby-Bauer disk diffusion method. Results: Out of total 100 strains of CoNS isolated, majority were in the age group of 15- 45 years (43%). Maximum isolates were from females 54(54%) than males 46 (46%). Maximum CoNS were isolated from blood (35%), followed by urine (33%), exudates (29%) and body fluids (3%). Most common species isolated was S. epidermidis (45%), followed by S. saprophyticus (22%), S. haemolyticus (13%), S. xylosus (5%), S. lugdunensis (4%), S. hominis (4%), S. capitis (4%) and S. cohnii (3%). Methicillin resistance was found in (61%) strains. Linezolid, Amikacin, Doxycycline, Gentamicin, Erythromycin, Norfloxacin and Ciprofloxacin were found to be the most effective antibiotics. Conclusion: S. epidermidis was the predominant species isolated. The most effective antibiotics were Linezolid and Amikacin.
Indian Journal of Medical Microbiology, 2016
established their role in infections of the bloodstream, urinary tract, surgical sites, prosthetic devices and shunts. Another concern is the rising incidence of methicillin-resistant (MR)-CoNS in hospitalised patients. [1] Resistance to β-lactams is determined by the mecA gene harboured on a mobile genetic element, i.e., staphylococcal chromosomal cassette mec (SCCmec). [2] This not only limits treatment options but also enables transfer of these resistance elements to other Staphylococci. It is imperative that the molecular characteristics of nosocomial MRCoNS isolates from India be elucidated to understand the mobilisation and evolution of these genetic elements Introduction Recent years have seen an increased recognition of coagulase-negative staphylococci (CoNS) as agents of hospital and community acquired infections. Previously dismissed as contaminants, these pathogens have now
JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH, 2022
Introduction: Coagulase Negative Staphylococci (CoNS) are common opportunistic pathogens. They are increasingly being recognised as nosocomial pathogens and are associated with multiple antimicrobial resistance mechanisms particularly methicillin resistance. Therefore, rapid and reliable identification upto the species level is necessary to predict the potential pathogenicity or antibiotic susceptibility of each clinical isolate. Aim: Isolation and speciation of CoNS from various clinical samples, and to determine their antibiotic susceptibility pattern. Materials and Methods: This study was a hospital-based crosssectional study carried out in the Department of Microbiology, Jawaharlal Nehru Institute of Medical Sciences (JNIMS), Imphal, Manipur, India, during the period from September 2017- August 2019. CoNS isolates were identified using conventional microbiological procedures and speciation was done following the scheme of Kloos and Schleifer. Antibiotic susceptibility was determ...