The use of fucosphere in the treatment of dermal burns in rabbits (original) (raw)
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Preparation of fucoidan-chitosan hydrogel and its application as burn healing accelerator on rabbits
Biological & …, 2008
Burn healing includes a specific biological process related to the general phenomenon of growth and regeneration and the primary objective in burn care is the promotion of rapid wound healing with the best functional results. The principal function of a burn dressing is to provide an optimum healing milieu for natural healing and desirable burn dressing may, therefore, be characterized on the basis of its performance such as; (a) provision of adequate gaseous exchange, (b) provision and maintenance of a moist environment, (c) protection from infections and contamination, (d) absorption of wound fluids and (e) painless and easy removal. Hydrogels are ideal biopolymeric pharmaceutical forms for the treatment of skin wounds. They have low interfacial tension, high molecular and oxygen permeability, good moisturizing and mechanical properties that resemble physiological soft tissue. 3,4) For this reasons, polysaccharides, e.g. chitosan, are having hydrogel forming properties have been considered to be advantageous in its application as a wound dressing material. 5) Chitosan, [a (1→4) 2-amino-2-deoxy-b-D-glucan], a unique polysaccharide derived from deacetylation of chitin, has been used in wound treatment owing to its good biocompatibility, biodegradability and accelerated granulation. 6-8) On the other hand, fucoidan is a sulphated polyfucose polysaccharide and has attracted considerable biotechnological research interest since the discovery that it possessed anti-coagulant activity similar to that of heparin and also reported to possess other properties including antithrombotic, anti-inflammatory, anti-tumoral and anti-viral effects. 9,10) Many of these effects are thought to be due to its interaction with growth factors such as basic fibroblast growth factor (bFGF) and transforming growth factor-b (TGF-b). Fucoidan may, therefore, be able to modulate growth factordependent pathways in the cell biology of tissue repair. 11) Although a great number of studies on different pharmacological properties of fucoidan and chitosan are present, there is little information on the fucoidan-based system used in burn healing and its only limited with cell culture. The aim of this study was to prepare fucoidan-chitosan hydrogel and to investigate its treatment efficiency on dermal burns on rabbits.
AAPS …, 2007
The aim of this study was to develop chitosan film containing fucoidan and to investigate its suitability for the treatment of dermal burns on rabbits. Porous films, thickness between 29.7 and 269.0 μm, were obtained by the solvent dropping method.Water vapor permeability (3.3-16.6/0.1 g), the swelling (0.67-1.77 g/g), tensile strength (7.1-45.8 N), and bioadhesion (0.076-1.771 mJ/cm2) of the films were determined. The thinnest films were obtained with the lowest chitosan concentration (P G .05). The water absorption capacity of the films sharply increased with the freeze-drying technique. The film having the thickness of 29.7 μm showed the highest amount of moisture permeability (16.6 g/0.1 g). Higher chitosan concentration significantly increased tensile strength of the films (P G .05). Using higher concentration of lactic acid made films more elastic and applicable, and these films were selected for in vivo studies. Seven adult male New Zealand white rabbits were used for the evaluation of the films on superficial dermal burns. Biopsy samples were taken at 7, 14, and 21 days after wounding, and each wound site was examined macroscopically and histopathologically. After 7 days treatment, fibroplasia and scar were observed on wounds treated with fucoidan-chitosan film. The best regenerated dermal papillary formation, best reepithelization, and the fastest closure of wounds were found in the fucoidan-chitosan film treatment group after 14 days compared with other treatment and control groups. It can be concluded that fucoidan-chitosan films might be a potential treatment system for dermal burns and that changing formulation variables can modulate the characterizations of the films.
Chitosan topical gel formulation in the management of burn wounds
International Journal of Biological Macromolecules, 2009
Wound healing properties of chitosan with different molecular weight and degree of deacetylation ranges have been examined. The macroscopic image and histopathology were examined using chitosan, Fucidin ® ointment and to blank. The rate of contraction was evaluated by determination of the unclosed area as a function of time. The treated wounds were found to contract at the highest rate with high molecular weight-high degree of deacetylation chitosan-treated rats as compared to untreated, treated, and Fucidin ® ointment-treated rats. Wounds treated with high molecular weight chitosan had significantly more epithelial tissue (p < 0.05) than wounds with any other treatment and the best re-epithelization and fastest wounds closure were found with the high molecular weight chitosan treatment group. Histological examination and collagenase activity studies revealed advanced granulation tissue formation and epithelialization in wounds treated with high molecular weight chitosan (p < 0.05). High molecular weight with high degree of deacetylation chitosan samples therefore demonstrates potential for use as a treatment system for dermal burns.
Biomedical Materials, 2016
In the biomedical sector the availability of engineered scaffolds and dressings that control and reduce inflammatory states is highly desired, particularly for the management of burn wounds. In this work, we demonstrate for the first time, to the best of our knowledge,that electrospun fibrous dressings of poly(octyl cyanoacrylate) (POCA) combined with polypropylene fumarate (PPF) possess anti-inflammatory activity and promote the fast and effective healing of mild skin burns in an animal model. The fibers produced had an average diameter of (0.8±0.1) µm and they were able to provide a conformal coverage of the injured tissue. The application of the fibrous mats on the burned tissue effectively reducedof around 80% in the first 48 hours the levels of pro-inflammatory cytokines in comparison with not-treated animals,and enhanced skin epithelialization.Specifically, the skin appeared similar to naïve animals in term of thickness (no burn: 13.7± 1.4µm versus burn+POCA:PPF: 14.3± 2.5µm), and dermal cells (no-burn 1100 ± 112 cells/mm 2 vs. burn+POCA:PPF 1358 ± 255 cells/mm 2). Furthermore, the reduction of the epidermis thickness and cell density was of around 99and 85%, respectively, compared to that of the injured untreated animals. Altogether, the results demonstrate the suitability of the dressing in accelerating and effectively promoting the burn healing process. 1.Introduction In the last years there has been a growing research interest in new functional materials that can be applied in the biomedical field.[1,2] In particular, in the regenerative medicine area, the development of advanced systems for the efficient treatment of skin wounds is becoming crucial in order to face global demographic issues (ageing population, obesity and diabetes).[3,4] Among the different types of wounds, skin burn is considered one of the worst trauma.[5] Typically, this kind of injury is treated by the following main actions: prevention and control of possible infections, absorption of fluids, modulation of hypermetabolic response, wound care and surgery.[5] Depending on the degree of burns, there are different approaches of management that can be adopted, ranging from the promotion of spontaneous re-epithelialization using powders, creams or dressings (for superficial and epidermal burns) to the application of autologous skin grafts (for full-thickness burns).[6] In all the cases, the
Clinical Experience with Chitosan Matrix and Cultured Fibroblasts for Burns
Central Asian Journal of Global Health, 2014
Introduction. Burns are an important public health challenge due to the frequency of getting burns in day-to-day life, occupational hazards, and catastrophes. Treatment of burns is complex and is associated with high morbidity and mortality. Duration and complexity of burn treatment require finding new ways of curing and rehabilitating burns. The result of burn treatment plays a significant role in post-traumatic status of a patient and his or her consequent adaptation in society. Chitosan is a natural safe non-toxic product compatible with human tissues, characterized by hydrosorbid, anticoagulant, antibacterial, and wound healing features. The study aims to show a clinical application of chitosan-pectin scaffold with cultured human skin fibroblasts in the treatment of deep burns.Methods. The substrate was prepared by dissolving 3% chitosan in 0.5N acetic acid, which was then mixed with 3% solution of pectin dissolved in distillated water. Chitosan film was formed in a Petri dish ...
2011
Topical antibiotics like Sodium fusidate, Framycetin are used to treat burn wounds. But whether they influence healing of wounds is not known. The aim of the experiment to compare the effect of Sodium fusidate, Framycetin of Apex Laboratories Chennai, with that of available market preparations on experimentally induced burn wound healing in Wistar rats. Partial thickness burn wounds was inflicted, on Wistar rats under ketamine (50mg/kg/i.p), by pouring hot molten wax at 80 ◦ C into a metal cylinder of 300 mm 2 circular opening placed on shaven back of the rat. Animals with partial thickness burn wounds were divided into 7 groups (n=10). Group I did not receive any drug and served as control group. Group II, III, IV, V, VI and VII received topical creams of Sodium fusidate A, Sodium fusidate B, Fucidic acid, Framycetin A, Framycetin B, and Framycetin respectively, twice a day for 21 days or till complete healing whichever was earlier. Sodium fusidate B and Framycetin A significantly ...
A dermal substrate made of collagen-GA-chitosan for deep burn coverage: First clinical uses
Clinical Materials, 1994
Infection is thegreatest problem in bum patients ana' fopical antimicrobial agents must be chosen with great care, especially when cultured skin is grafted. We examined the cytotoxic effect of six antiseptics and six antibiotics commonly used on cultured human @rob&s and kerafinocytes. Cultured cells were exposed for 15 min to Hibitant? (chlorhexidine), Bisepfi&@ (chlorhexidine + benzalkonium chloride + benzylic alcohol), Benzalkonium Chloride, Yellow Betadine (polyvidone-iodine + nonoxinol), Betadine Scrub (polyvidone-iodine + quaferna y ammonium) and Green Betadine (polyvidone-iodine) and uiabilify ~a.5 determined using ihe MTT test. At therapeutic concentrations all the antiseptics are cytotoxic for fibroblasts and keratinocytes. Additionally the cells were exposed for 48 h to uancomycin, colistin, amikacin, imipeneme, pefloxmin, piperacillin and cell viability was determined using the MTT test. The concentration.5 of antibiotics corresponding to the plasma peak obtained after therapeutic appliction were not cytotoxic to the tested cells. The CD50 was much higher than the MlC from 725 to 875 times for keratinocytes and from 1400 to 5900 times for fibroblasts). These data suggest that commonly applied antiseptics must not be used before grafting cultured skin grafts. After grafting any infection can be controlled with topical applications of appropriafe antibiotics.
Topical antibiotics like sodium fusidate, framycetin and calcium mupirocin are used to treat burn wounds. But whether they influence healing of wounds is not known. Aim: to compare the effect of sodium fusidate, framycetin and calcium mupirocin of Apex Laboratories Chennai, on experimentally induced burn wound healing in male Wistar rats and compare with available market preparation namely, Fucidin cream 2% (ranbaxy), T Bact cream (GSK) & Soframycin cream (Sanofi-aventis). Method: Partial thickness burn wounds was inflicted, on Wistar rats under pentobarbitone anesthesia (30mg/kg/i.p), by pouring hot molten wax at 80 • C into a metal cylinder of 300 mm 2 circular opening placed on shaven back of the rat. Animals with partial thickness burn wounds were divided into 7 groups (n=10). Group 1 did not receive any drug and served as control group. Group 2, 3, 4, 5, 6, 7 received sodium fusidate, framycetin, calcium mupirocin, Fucidin 2% (ranbaxy), T Bact (GSK) and Soframycin (Sanofi-aventis) creams topically respectively twice daily for 21 days or till complete healing whichever was earlier.