Sensitive Immunoluminometric Assay for the Detection of Procalcitonin (original) (raw)
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A new sensitive automated assay for Procalcitonin detection: LIAISON® BRAHMS PCT® II GEN
Practical Laboratory Medicine, 2016
and assessing its ability to discriminate between healthy subjects and patients with suspected infection. Methods: Diagnostic performance was evaluated on: a) 193 selected samples covering the assay range, whose procalcitonin levels were already evaluated with the B Á R Á A Á H Á M Á S PCT s KRYPTOR; b) prospective samples: 150 apparently healthy specimens obtained from a blood bank, 161 hospitalized patients (not with specific pathologies), 243 apparently healthy children. Results: The comparison of LIAISON s BRAHMS PCT s II GEN to the reference method B Á R Á A Á H Á M Á S PCT KRYPTOR yielded high correlation coefficients: slope of Deming fit equal to 1.04 (95% CI: 0.99-1.09) with an intercept equal to 0.05 (95% CI: À 0.09 to 0.19) and a high concordance (98.4% (95% CI: 95.5-99.7%)) at the 0.5 ng/mL cutoff. Moreover, the results obtained using prospective samples showed: (i) no samples with PCT concentration 40.5 ng/mL (cut-off) for the apparently healthy adults (highest value ¼ 0.033 ng/mL, 95th percentile and 97.5th percentile o 0.02 ng/mL); (ii) 2 samples 4 0.5 ng/mL for hospitalized patients (highest value¼ 0.715 ng/mL, 95th percentile: 0.054 ng/mL; 97.5th percentile: 0.088 ng/mL); (iii) 3 samples 4 0.5 ng/mL for the healthy children population (highest value ¼ 0.713 ng/mL, 95th percentile: 0.155 ng/mL; 97.5th percentile: 0.275 ng/mL). Conclusion: The fully automated LIAISON s BRAHMS PCT s II GEN agrees well with the reference method and is suitable for early diagnosis of sepsis, severe bacterial infection and guiding antibiotic therapy.
Procalcitonin as a marker of sepsis
International Journal of Antimicrobial Agents, 2002
Prompt diagnosis and treatment with appropriate antimicrobial chemotherapy is of paramount importance to reduce morbidity and mortality associated with sepsis. Inflammatory markers currently in use, such as C-reactive protein (CRP) do not reliably differentiate between the systemic inflammatory response and sepsis. Procalcitonin (PCT), a precursor of calcitonin, is a 116 amino acid protein that has been proposed as a marker of disease severity in conditions such as septicaemia, meningitis, pneumonia, urinary tract infection (UTI) and fungal and parasitic infection. In particular, serial measurements are useful in order to monitor response to therapy. Together with good clinical judgement and judicious use of antimicrobial agents, PCT should serve as a valuable adjunct in the diagnosis and management of sepsis.
https://www.ijrrjournal.com/IJRR\_Vol.8\_Issue.4\_April2021/IJRR-Abstract057.html, 2021
Sepsis is the occurrence of infection in association with the systemic inflammatory response syndrome (SIRS). Bacterial sepsis is one of the major illnesses that shows severe symptoms which leads to organ dysfunction. Procalcitonin (PCT) the precursor of the hormone calcitonin shows a huge impact on the diagnosis of sepsis caused by the bacterial infection. Many studies have also insisted the importance of PCT as a biomarker for sepsis. As early diagnosis is the key factor to overcome the difficulties of the sepsis, PCT acts a prominent diagnostic tool to acquire the desired results. This study focused on the role of PCT in correlation with supporting parameters of the vital organs such as renal markers, liver enzymes and pancreatic enzymes to reduce the organ dysfunction and its complications.
The Use of Procalcitonin in Clinical Practice
Proceedings of Singapore Healthcare, 2014
Procalcitonin (PCT) has emerged as the most promising marker of infectious inflammation. This development is critical to the practicing doctor dealing with febrile patients with suspected sepsis. An ideal biomarker would provide data for early diagnosis, differentiation of bacterial from non-bacterial causes of inflammation and information about the clinical course and prognosis of the disease. PCT is an early biomarker that is present within 3 to 4 hours of the triggering infection. An undetectable PCT level would efficiently rule out systemic infection. PCT may also be viewed as a marker of resolving infection as it has a half-life of about 22 hours, and its blood level correlates with bacterial load. Thus, PCT may be used as a clinical tool for early diagnosis, prognosis and therapeutic guide. Automated platforms with short assay times and service that is available 24 hours a day have enabled clinicians to obtain rapid reliable results for the early diagnosis and timely monitorin...
Procalcitonin (PCT) in patients with abdominal sepsis
Intensive Care Medicine, 2000
Background: To assess the accuracy of procalcitonin as a measure of severity in patients with septic abdominal illnesses and the sepsis syndrome, to compare measurements with those of other inflammatory mediators, and to predict outcome.¶Methods: We carried out a prospective clinical study from 246 patients with infective or septic episodes confirmed at laparotomy and 66 patients undergoing elective operations who acted as controls. Specimens of blood for measurement of cytokine concentrations determination were obtained daily from septic patients. In the control group specimens were obtained before operation, at the end of operation, and on each of the following days until normal recovery (day 10). Every two weeks up to 3 months for patients with metastases, who were being followed up.¶Results: Compared with other cytokines such as tumor necrosis factor αa and interleukin 6 procalcitonin was closely related to the development of infective and septic complications. 59 of 246 patients (24 %) with sepsis died. Procalcitonin concentrations preoperatively [median 2.05 compared with 4.2 ng/ml (p = 0.08)] (Mann-Whitney U-test) did not differ, but those on the days 1, 4 and at the end differed significantly [day 1: 4.9 compared with 13.8 ng/ml (p Conclusions: Procalcitonin is a new indicator of infection and sepsis. TNF and IL-6 concentrations always rise after major operations and fall in the absence of infection, indicating operative trauma. Procalcitonin is sensitive in detecting infective complications. Under routine conditions the procalcitonin concentrations seems to be valid, reproducible and detectable.
Clinical Chemistry and Laboratory Medicine (CCLM), 2016
Procalcitonin (PCT) is increasingly being used for the diagnostic and prognostic work up of patients with suspected infections in the emergency department (ED). Recently, B·R·A·H·M·S PCT direct, the first high sensitive point-of-care test (POCT), has been developed for fast PCT measurement on capillary or venous blood samples.This is a prospective, international comparison study conducted in three European EDs. Consecutive patients with suspicion of bacterial infection were included. Duplicate determination of PCT was performed in capillary (fingertip) and venous whole blood (EDTA), and compared to the reference method. The diagnostic accuracy was evaluated by correlation and concordance analyses.Three hundred and three patients were included over a 6-month period (60.4% male, median age 65.2 years). The correlation between capillary or venous whole blood and the reference method was excellent: rThis study found a high diagnostic accuracy and a faster time to result of B·R·A·H·M·S P...