Degradation and decolourization of textile dyes effluents (original) (raw)
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Biodegradation of textile dyes by bacteria isolated from textile industry effluents
Stamford Journal of Microbiology
A country like Bangladesh where textile industries are the main source of developing economy, pollution problem from such industries creates a huge risk for the environment. Textile industries discharge a huge amount of effluent containing various harmful chemicals including synthetic dyes that are very stable and threat to the living organisms. This study deals with the potential decolorization and biodegradation of Bemacron Yellow HP-2R (BY), Bemacron Red RS (BR) and Bemacron blue RS 01 (BB) dyes using bacteria isolated from textile effluent. The effluent and soil samples were collected from different locations of discharge point. Only two isolates were screened out after primary screening using dye supplemented nutrient agar media. Following colony morphology, physiology and biochemical analysis, they were presumptively identified as Bacillus sp. and Staphylococcus aureus. They were subjected to decolorization of 0.002 g/l BY, BR and BB dyes. Bacillus sp. showed superior decolori...
Biodegradation of Synthetic Dyes by Bacteria Isolated from Textile Industry Water Effluent
Journal of Advances in Biology & Biotechnology
Aims: Biodegradation of synthetic dyes by bacteria isolated from textile industry water effluent. Study Design: Different bacterial strains were isolated from the water effluent generated by textile industry. These bacteria were identified using morphological and molecular characterization and their ability to degrade synthetic dyes was evaluated. The dyes used in this study were methylene blue, malachite green, congo red, and methyl red. Place and Duration of Study: Samples were collected from textile industrial area of Mandideep, Raisen (Madhya Pradesh, India). All the experiments were conducted in Department of Microbiology, Barkatullah University, Bhopal (Madhya Pradesh, India) between January 2019 to June 2019. Methodology: Textile dye effluent was collected from industrial area of Mandideep, Raisen (Madhya Pradesh, India). The collected effluent sample was analyzed for its physical characteristics (pH, temperature, colour, odour, electrical conductivity (EC), total dissolved s...
2020
Textile industries are responsible for one of the major environmental pollution problems in the world, because they release undesirable dye effluents. Textile wastewater contains dyes mixed with various contaminants at a variety of ranges. Therefore, environmental legislation commonly obligates textile factories to treat these effluents before discharge into the receiving watercourses. The present study was an attempt to examine the Decolorization ability of Prominent isolates obtained from the Industrial Effluent Sample (Kocuria rhizophila and Bacillus cereus) for two different commercial Textile Grade Dyes that are ‘Perssian blue’ and ‘Reactive orange 13’. The primary parametric study was done and results were recorded. The isolates showed significant decolorization of both the dyes was identified by bacterial morphology, biochemical tests and also with of Automated Microbial Identification System (VITEK 2) instrument. Various physico-chemical parameters with respect to incubation...
International Journal of Advanced Scientific Research and Management
Pseudomonas aeruginosa and Brevibacillus choshinensis were enriched and isolated from effluent sample of a textile processing unit using Sterile Minimal medium containing 10% Glucose and incubated at 28 ± 2° C for 7 days. The isolates were identified using standard biochemical tests carried out by KEM Hospital, Pune, India. These isolates were checked for decolourisation of textile dyes and biological stain solutions. It was found that both the bacterial isolates were able to degrade the textile dyes upto 250 ppm concentration. Pseudomonas aeruginosa was further studied for decolourisation of biological stain solutions and was found to decolorize upto 100 ppm of the stain solution. Phytotoxicity of the textile dyes was estimated by measuring the relative changes in seed germination of three plants: Triticum spp. (Wheat), Vigna radiate (Moong seeds) and Brassica juncea (Mustards seeds). Radicle and Plumule growth (length) were recorded after 6 days of exposure to different concentrations of dyes. Toxicity of the degraded dye was also checked which indicated that after decolourization the toxicity of the dye reduced. This property of these bacterial isolates shows a potential that can be utilized for the bioremediation of various textile industrial effluents thus saving the ecosystem from harmful effects of various dyes.
Isolation and Characterization of Dye Decolorizing Bacteria from The Textile Dye Effluents
Journal of Advanced Zoology, 2023
The most significant challenge confronted by the textile industries is the discharge of dye effluents which contains toxic chemicals posing a considerable threat to environmental pollution. Biological method of treating effluents using bacteria is one of the renewed and accepted method in effluent treatment. The present study aims to isolate and characterise the dye decolorizing bacteria from the effluent samples of a dye industry from Chinnalapatti, Tamilnadu. The dye effluents were collected from the effluent plant of a leading dye industry and the Physiochemical parameters were analysed. The samples were plated in a Nutrient Agar and bacterial isolation were performed according to the standard microbiological procedures. The bacterial isolates identified by biochemical identification were tested for its efficiency to decolorize Methyl red and crystal violet dyes. Among the three isolates S1showed 98% decolorizing efficiency in reducing Methyl red dye and 92% efficiency in decolorizing crystal violet dye. Sample S2 showed 85% activity in decolorizing methyl red dye and 86% activity in decolorising Crystal violet dye. S3 showed a low decolorizing activity compared to S1 and S2. Both of these phenotypically identified isolates are subjected to molecular identification by 16s gene amplification and gene sequencing. The obtained FASTA sequence were analysed for the phylogenetic relationship and submitted to NCBI. The potential isolate that has the capacity to decolorise the dyes were identified as Bacillus Tropicus and Pseudomonas mosselli. This study proves that these bacterial strains can be used as a potential bioremediation agent in decolorizing the dye effluents.
Bacterial decolorization is efficient and fast, but individual bacterial strains usually cannot degrade azo dyes completely and the intermediate products are often carcinogenic aromatic amines, which need to be further decomposed. Thus, treatment systems composed of mixed microbial populations achieve a higher degree of biodegradation and mineralization due to the synergistic metabolic activities of the microbial community and have considerable advantages over the use of pure cultures in the degradation of synthetic azo dyes. In the present study, dye house effluent was collected from a dyeing unit in Theco Silks, Thirubhuvanam region, Kumbakonam district, Tamil Nadu, India. It was refrigerated at 4°C and used without any preliminary treatment. Six different bacterial isolates viz., Bacillus odyssey, Bacillus thuringiensis, Bacillus subtilis, Bacillus cereus, Alcaligenes sp. and Nocardiopsis alba were isolated from the textile dye effluent sample. The bacterial isolates were isolated by Serial dilution technique (Pour plate method). The bacterial isolates were identified in genus level by the Colony morphology, Preliminary tests like Gram staining, Capsule staining, Endospore staining, Motility, Catalase and Oxidase, Plating on selective medias and by performing biochemical tests. The identified bacterial isolates were screened for its decolourization of Reactive dyes by Plate assay. The bacteria Nocardiopsis alba showed maximum zone formation when compared to other bacterial isolates.
Biodegradation of textile dye effluent through Indigenous bacteria
2020
The textile industry is considered as one of the major generators of toxic chemical wastewater in India. Dyes released by the textile industries pose a threat to environmental safety. Dye decolorization through biological means has gained momentum as these are cheap and can be applied to a wide range of dyes. The present study concentrates in the isolation, identification of indigenous bacteria namely D1, D2, D3 and D4 from textile dye effluent collected from the local textile dyeing shop located at Gurahakuan, Banda district, Uttar Pradesh, India, and evaluation of their ability to decolorize dyes sample. The isolated bacteria were identified through morphological and biochemical characteristics. Scanning electron microscope (SEM) analysis of isolated bacteria showed that all the bacteria appeared rod-shaped with size ranging from 1.33 to 2.84 µm. The physico-chemical analysis of dye effluent indicated the bluish-black color of the effluent having pH of about 8. The Biological Oxyg...
Asian Journal of Chemistry
Textile wastewater is the major problem in the world as production of colorant reached 8 × 10 6 kg per year, of which 10-15 % goes into effluents during different textile processes 1,2. These colorants are primarily designed to resist fading of colour in the presence of intense light and moisture conditions to make good quality fabric. Discharge of such dyes in wastewater is a great threat to the natural ecosystems because these are toxic as well as carcinogenic. Thus, there is a dire need to develop a cost effective strategy to deal with such dyes which are harmful to life 3,4. In this scenario, biological processes to treat such dyes could be environment friendly and cost effective procedures 5. For example, degradative enzymes can be utilized to degrade textile effluents. These enzymes have low energy requirement, minimum operation cost and eco-friendly nature which make them suitable for such treatments. Laccases are the enzymes which are used for biotreatment of dyestuffs. Information regarding effluent treatment by lacases is available in the literature but potential of other enzymes such as oxidases and reductases is yet to be explored 1 .
Bacterial Degradation of Synthetic Dye by Pseudomonas sp. Obtained from Dyeing Mill Effluent
American Journal of Pure and Applied Biosciences
Environmental pollution is one of the major concerns of today’s world. Due to rapid industrialization and urbanization, a large number of wastes are generated and discharged into the environment and causing major pollution problems. For obtaining reactive dye decolorizing bacterial isolates, effluent samples were collected from a dyeing mill. From bacterial pure culture, 10 isolates were selected for screening. Screening of these isolates for the capability to decolorize and degrade reactive dye was performed in a nutrient broth medium containing reactive dye. 6 isolates among these bacterial isolates showed dye decolorizing ability within 120 hours of incubation. These isolates were further identified based on cultural, morphological, and biochemical characteristics. These characteristics indicated that these six bacterial isolates were distributed to the bacterial genus of Bacillus (2 isolates), Pseudomonas (2 isolates), Aeromonas (1 isolate), and Alcaligenes (1 isolate). For the ...
Journal of Applied Sciences and Environmental Management, 2011
The evaluation of some microbial species for the decolourization and degradation of textile dye has been investigated. Six microbial strains were isolated from soil contaminated with textile waste effluents using the spread plate technique and the isolates were identified as bacterial isolates (Pseudomonas fluorescence, Pseudomonas nigificans, and Pseudomonas gellucidium) and fungal isolates ( Aspergillus niger, Proteus morganii and Fusarium compacticum} based on gram staining, morphological and biochemical tests. They were evaluated for their capability to remove colour and degrade dye, reduce chemical oxygen demand (COD) and biological oxygen demand (BOD) levels of textile waste effluents. The results revealed that all the bacterial and fungal isolates have a good potential to remove colour and degrade dye, reduce the COD and BOD levels of the textile waste effluents with percent colour removal, COD and BOD reductions between 39 and 48%, 74 and 97% and 77 and 95%, respectively. Binary mixed culture of Pseudomonas fluorescence and Aspergillus niger was efficiently utilized for the removal of different initial concentration (10, 15, 20, 25 and 30 mg/l) of dye from textile waste effluents. It had a higher percent decolourization than individual isolates. The stirred tank bioreactor was found to be very effective for efficient biotreatment of textile waste effluents. @JASEM