Evaluation of Antimicrobial Potentiality of Aqueous Ethanolic Leaf Extract of Acacia Arabica Extract (original) (raw)
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Antimicrobial Activity of Bark extract from Acacia
Acacia jacquemontii was assessed for active principles to ascertain the rationale for its use in traditional medicine. Preliminary phytochemical screening of the stem bark extracts showed that it possessed the active principles -alkaloids, glycosides, saponins, terpenoids and tannins. The antimicrobial activity of the extracts was assayed against pathogenic strains of Bacillus cereus, Bacillus pumilus, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, S. pyrogenes, and Candida albcans using the agar diffusion method. The plant extract exhibited antimicrobial activity against all the test microorganisms. B. cereus and B. pumilus were the most susceptible to the plant extract while Candida albicans was the most resistant. The minimum inhibitory concentration of the stem bark extract of the plant ranged between 30 and 50 mg/ml while the minimum bactericidal concentration ranged between 35 and 60 mg/ml. A. jacquemontii could be a potential source of antimicrobial agents.
African journal of traditional, complementary, and alternative medicines : AJTCAM, 2016
In Europe, Acacia and Eucalyptus, originate large amounts of biomass, due to their need by industries and other biological control, that can be used to extract antimicrobial substances. Foliar aqueous, ethanolic and methanolic extracts of Acacia baileyana (Cootamundra wattle), Acacia dealbata (silver wattle), Acacia melanoxylon (black wattle) and Eucalyptus nicholii (narrow-leaved black peppermint) were assessed for antimicrobial activity against Escherichia coli, Bacillus cereus, Candida albicans and Candida parapsilosis, using the disc diffusion method. Ethanolic extracts from A. baileyana and A. dealbata showed significant (P< 0.05) antimicrobial activity. Concerning the microbial species tested, differences were found in A. baileyana (P< 0.01) and E. nicholii (P< 0.0001) extracts. These two extracts were effective mostly against B. cereus, followed by C. parapsilosis. According to the antimicrobial activity classification, eucalypt and Cootamundra and silver wattles ext...
Antibacterial activity of ethanolic and aqueous extracts of Acacia aroma Gill. ex Hook et Arn
Life Sciences, 2004
The purpose of the present study was to investigate the antibacterial activity of seven ethanolic extracts and three aqueous extracts from various parts (leaves, stems and flowers) of A. aroma against 163 strains of antibiotic multi-resistant bacteria. The disc diffusion assay was performed to evaluate antibacterial activity of the A. aroma crude extracts, against several Gram-positive bacteria (E.
Journal of Biomedical Research & Environmental Sciences, 2021
The antimicrobial activity about water extract of Acacia etbaica was examined by using agar well diffusion methods against five gram-positive and negative bacteria[Staphylococcus aureus (ATCC653-8), Pseudomonas aeruginosa MTCC2453, Bacillus cereus (ATCC6633), Escherichia coli MTCC739, and one local isolate (Staphylococcus epidermides)] in addition to Candida albicans (ATCC2019). this results designated that the water extract of Acacia etbaica possess antimicrobial efficacy against all tested microorganisms either (gram-positive and negative bacteria) or fungi (Candida albicans).Whereas the gram-positive bacteria (S. aureus, S. epidermides and B. cereus) with inhibition zones (21, 19.5 and 16.5) respectively was more sensitive than gram-negative bacteria (P. aeruginosa and E. coli) and C. albicans with inhibition zones 16 and 14.5 respectively. The antimicrobial effect was directly proportional with concentration of water extract where the highest inhibition zone at higher concentrat...
Advances in Microbiology, 2016
The ethanol, chloroform and acetone extracts of five species from Acacia (Acacia albidia stems, Acacia mellifera aerial parts, Acacia nubica aerial parts, Acacia seyal var. seyal stems and Acacia tortilis aerial parts) were investigated for their antimicrobial activity against two standard bacterial strains of Gram +ve bacteria (Staphylococcus aureus (ATCC 25923)), Gram −ve bacteria (Pseudomonas aeruginosa (ATCC 27853)) and standard fungi Candida albicans (ATCC 90028) using the agar-plate well diffusion method. The chloroform extract was inactive compared to ethanol and acetone extracts. But ethanol extracts showed the maximum antimicrobial activity against the test organism. Amongst the plant species screened, ethanol extract of Acacia seyal stems showed maximum inhibitory activity (38 mm) and (37 mm) against Staphylococcus aureus and Candida albicans, respectively. The ethanol, chloroform and acetone extracts of Acacia mellifera (aerial parts) did not show any activity against the test organisms. Cholorophorm and acetone extracts via DPPH, the radical scavenging activities were found to be 91 ± 0.03, 88 ± 0.01 and 85 ± 0.04, respectively. The results of phytochemical screening showed that all extracts of studied plant contain flavonoids, saponins, terpenoids, steroids, alkaloids, phenols and tannins. H. B. Abdllha et al.
Anti-bacterial Activity of Methanolic Fruits Extract of Acacia nilotica (L.)
Acacia nilotica (L.) is used to treat different ailments for instance cold, congestion, fever, gallbladder, hemorrhage, hemorrhoids, leucorrhoea, ophthalmic, sclerosis, small pox, intestinal pains and acute diarrhea. Other preparations are used for gargle, toothache, ophthalmic and syphilitic ulcers. The fruits of A. nilotica were tested against eight standard bacterial species: two Gram-positive bacteria viz, Enterococcus feacalis and Staphylococcus aureus, six Gram-negative bacterial strains Escherichia coli, Pseudomonas aeruginosa, Shigella Sonnei, Salmonella Para A, Klebsiella pneumoniae and Proteus mirabilis using the disc diffusion method. Antibacterial activity of fixed oil of A. nilotica dissolved in methanol (1:10), showed high activity against the Gram-negative bacteria (P. aeruginosa & E. coli) (18 & 14 mm). It also showed against Gram positive bacteria (S. aureus & B. subtilis) (14 & 13 mm) and against (C. albicans) (14 mm). This study conducted of A. nilotica fruits proved to have potent activities against antibacterial activity in vitro.
2015
This study was conducted to determine the in-vitro anti-microbial activity of Acacia etbaica, native plant to east African countries, against Staphylococcus aureus and Escherichia coli. To achieve this, methanol extract of leaf of Acacia etbaica was tested for its in-vitro antibacterial activity against Staphylococcus aureus and Escherichia coli using agar disc diffusion method at two different concentrations (500μg/disc and 1000μg/disc). The Minimum Inhibitory Concentration (MIC) of the plant crude extract was also determined using micro-dilution method in 96-well plates. Acacia etbaica showed significant antibacterial activity with mean zone of inhibition of 13.34 ± 1.04 mm and 11.13 ± 1.04 mm in diameter at a concentration of 1000μg of plant extract per disc against S. aureus and E. coli respectively. The MIC of the crude extracts of Acacia etbaica was determined to be 0.039mg/ml and 0.313mg/ml against S. aureus and E. coli respectively. The results suggest that the methanol extr...
In Vitro Antimicrobial Activity of Acacia catechu and Its Phytochemical Analysis
Indian Journal of Microbiology, 2010
Acacia catechu, commonly known as catechu, cachou and black cutch is an important medicinal plant and an economically important forest tree. The methanolic extract of this plant was found to have antimicrobial activities against six species of pathogenic and non-pathogenic microorganisms: Bacillus subtilis, Staphylococcus aureus, Salmonella typhi, Escherichia coli, Pseudomonas aeruginosa and Candida albicans. The maximum zone of inhibition (20 mm) was found to be exhibited against S. aureus. For this organism the minimum bactericidal concentration (MBC) of the crude extract was 1,000 μg/ml. The extract was found to be equally effective against gram positive and gram negative bacteria. The antimicrobial activity of the extract was found to be decreased during purification. The chemical constituents of organic plant extracts were separated by thin layer chromatography (TLC) and the plant extracts were purified by column chromatography and were further identified by Gas chromatography–mass selection (GC–MS) analysis. The composition of A. catechu extract had shown major components of terpene i.e. camphor (76.40%) and phytol (27.56%) along with other terpenes in minor amounts which are related with their high antibacterial and antifungal properties.
Journal of pharmacopuncture, 2013
Evaluations of the in-vitro anti-bacterial activities of aqueous extracts of Acacia catechu (L.F.)Willd, Castanea sativa, Ephedra sinica stapf and Shilajita mumiyo against gram-positive bacteria (Staphylococcus aureus, Streptococcus pneumonia) and gram-negative bacteria (Escherichia coli, klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa) are reasonable since these ethnomedicinal plants have been used in Persian folk medicine for treating skin diseases, venereal diseases, respiratory problems and nervous disorders for ages. The well diffusion method (KB testing) with a concentration of 250 μg/disc was used for evaluating the minimal inhibitory concentrations (MIC). Maximum synergistic effects of different combinations of components were also observed. A particular combination of Acacia catechu (L.F.) Willd, Castanea sativa, Ephedra sinica stapf and shilajita mumiyo extracts possesses an outstanding anti-bacterial activity. It's inhibiting effect on microorganisms ...
2011
Aim: The Objective of Our study is to investigate the invitro antibacterial activity of ethanolic bark extract of Acacia catechu willd (fabaceae) against Enteric pathogens. Materials and Methods: The inhibitory effect of ethanolic bark extract of Acacia catechu willd was tested against five Gram negative bacilli and one Gram positive cocci by using the Disc diffusion method and the MIC & MBC values were determined. Results: The ethanolic bark extract exhibited antibacterial activity against staphylococcus aureus and shigella dysentriae with minimum inhibitory concentration, 1 mg/ml and minimum bactericidal concentrations of 1mg/ml. Conclusion: The ethanolic bark extract of Acacia catechu willd was found to be bactericidal in action. *Corresponding author, Mailing address: Ms. Lakshmi.T Senior Lecturer, Pharmacology, Saveetha Dental College & Hospitals, Chennai. Email: lakshmi085@gmail.com Article History:-----------------------Date of Submission: 02-09-2011 Date of Acceptance: 25-09...