An Evaluation of the Oral Microbiome and Potential Zoonoses of the Southern Thick-Tailed or Greater Galago ( Otolemur crassicaudatus ) (original) (raw)

As the southern thick-tailed or greater galago (Otolemur crassicaudatus) is ubiquitous throughout parts of southern Africa they are thought to be of little conservation risk. As such, this species is seldom studied and relatively little is known of their behavior and biology. This research partially addresses this lack of knowledge by investigating the oral microbiome for potential zoonoses. Real-time PCR was used for the detection of Mycobacterium tuberculosis and M. leprae whereas next generation MiSeq Illumina sequencing was conducted on the 16S ribosomal RNA gene (16S rRNA) with a universal primer set which amplified the variable V3 and V4 regions to quantify the amount of different bacterial taxa. Major bacterial taxa of each tested oral microbiome were isolated, and those with potential for causing disease in humans and domestic animals were identified. The results illustrated a potential core microbiome for all O. crassicaudatus consisting of three separate bacterial taxa: Ma...

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