Preliminary phytochemical screening for various secondary metabolites, quantitative and qualitative analysis of Yemeni brown seaweed Sargassum vulgare (original) (raw)
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Preliminary phytochemical analysis of the ethanolic extract of brown Seaweed Sargassum wightii
The big stores for the favorable algae are the Seas and Oceans and the study of seaweeds is called as phycology or ecology. The identification and isolation of new substances are growing from the source of marine organisms. The seaweeds live in salty water and are eukaryotic organisms considered as a good source of bioactive natural products. The present study investigated to explore the phytochemical constituents of the seaweed Sargassum wightii (Brown algae). The brown marine algae " Sargassum wightii " belongs to the family " Phaeophyceae ". Ethanol was used as a solvent system for the preparation of the extract of Sargassum wightii. The ethanolic extracts of Sargas-sum Wightii were undergone to the qualitatively phytochemical test by means of typical measures. Phytochemical analysis shows the presence of alkaloids, tannins, steroids, flavonoids, and carbohydrates, whereas proteins, free amino acids and saponins were found to be absent. The results of the study may lead a foundation for t he further studies on this marine algae Sargassum wightii.
Preliminary Phytochemical Analysis of the Crude Extract of Sargassum Wightii
2020
Seaweeds are marine macroscopic algae which form an important component of marine living organisms. In recent years, the secondary metabolites (phytochemicals) have been extensively investigated as a source of medicinal agents. Sargassum wightii growing in tropical and sub tropical regions have been known for a wide range of biological activities. The aim of the present study was to perform the phytochemical analysis of secondary metabolites both qualitatively and quantitatively of the three different extracts of Sargassum wightii. The preliminary phytochemical analysis revealed the presence of alkaloids, glycosides, steroids, tannins, flavonoids, proteins and phenolic compounds and saponins was found to be absent. Among the three different extracts, methanol extract showed the presence of maximum number (7) of compounds. The estimation of total phenolics, tannins and flavonoids were observed in different extracts of Sargassum Wightii. The results indicated that the maximum phenolic...
Journal of Oleo Science
logical activities of Sargassum extract or purified compounds include anticancer, antibacterial, antifungal, antiinflammatory, anticoagulant, antioxidant, hypoglycemic, hypolipidemic, and hepatoprotective 3. Sargassum genus is widely spread in various water areas in Indonesia. Also, it has promising biomass potential because of the good growth in a tropical area. Since the chemical composition of seaweeds is strongly influenced by their environmental conditions 5 10 , the evaluation of different marine habitats on seaweed chemical content is important to be conducted. This is related to the bioprospecting strategy for seaweed industry development, especially for future functional ingredient sources. This
2020
The demands for increasing anticancer drugs produced by natural sources with potential antioxidant and antimicrobial properties had led the path to mass production in pharmaceutical fields. This current investigation is implied to extract the potential crude secondary metabolites extract from brown algae Sargassum wightii Greville ex J.Agardh with maximum antimicrobial and antioxidant properties. The brown seaweed was collected from Kanyakumari, Tamil Nadu, India and taken for extraction process using various polar and non-polar solvents. The solvents which extracted maximum crude metabolites were taken to determine the preliminary antimicrobial analysis using clinical pathogens on which the crude extracts explored the highest zone of inhibition at least concentration. Further, the five different crude extracts were subjected to antioxidant assessment and the extract in different solvents manifested maximum activity at minimum concentration. The results of antimicrobial and antioxid...
2011
During our search program for bioactive compounds from Egyptian marine sources, we isolated a spatane diterpene tetraol (1), fucosterol (2) and linoleic acid (3) from the brown alga Sargassum subrepandum (Forsk) C.Ag. In addition to those compounds, the four hydrocarbons were detected by using GC-MS named heptadecane, 2,6,10,14-tetramethyl-hexadecane, nonadecane and heneicosane. The chemical structure of 1 is assigned here definitely to first time by spectroscopic analyses including mass spectrometry (EI-MS, HR/EIMS), 1D and 2D NMR experiments. Phytochemical study of the unsaponified fraction of the algal extract and analysis by GC-MS confirmed the existence of nine compounds, among them 4,4,7a-trimethyl5,6,7,7a-tetrahydrobenzofuran-2[4H]-one (6). In this article, we report as well the biochemical composition of Sargassum subrepandum (including ash, fibre, lipid, carbohydrate, total protein, and amino acids compositions) together with biological activities of its extract and the iso...
Jurnal Perikanan Universitas Gadjah Mada
Sargassum polycystum is one brown seaweed with a rich alginate source. The previous study showed that S. polycystum could be used as a cosmeceutical raw material because of its natural attribute and safe. S. polycystum always grows in many coastal of Indonesia. This study aims to characterise brown seaweed (S. polycystum) as the raw material of the body scrub. The research method includes the extraction, phytochemical assay, antioxidant assay, and total phenolic content-the phytochemical screening-detected alkaloid and steroid compounds in S. polycystum extract. The antioxidant activity by using the CUPRAC method showed the ethanol extract was 53.55±1.07 µM/g and FRAP was 201.95±6.33 µM/g with total phenolic content was 177.647±21.39 Mg GAE/g. We compared the antioxidant capacity of the body scrub by adding brown seaweed of S. polycystum 7% (S7) used the CUPRAC method was 34.23±0.33 µM/g, and FRAP was 124.05±1.87 µM/g with total phenolic content was 416.31±78.44 Mg GAE/g. Kruskal Wa...
2013
The use of macroalgae has grown exponentially during the last decade. In addition to their value for human nutrition, seaweeds have multiple therapeutic applications. This work is aimed at providing information on the three least studied species of Sargassum so as to promote these algae to be potentially profitable from biotechnology and commercial perspectives, and also benefit public health. The proximate composition, total phenolic content, total antioxidant activity, alginic acid yield of three brown seaweeds, Sargassum myriocystum, S. plagiophyllum and S. ilicifolium were studied. The seaweeds were high in carbohydrate (33.38-40.21%) and low in lipid content (0.17-0.4%) on dry wt basis. Total phenolic content and total antioxidant activity were high in Sargassum plagiophyllum (28.66 ±3.05 mg /g) and S.ilicifolium (36.66 ± 9.86 mg /g). These seaweeds showed (12.6-15.0%) alginic acid yield. The characteristic peaks of alginic acid appeared at 3414.00, 3491.16, 3414.00, 1635.64, 1...
Food Chemistry Advances, 2024
Seaweeds possess considerable potential for developing and enhancing pharmaceutical drugs because of their wide variety as abundant sources of numerous bioactive secondary metabolites, making them well-suited for developing and enhancing pharmaceutical drugs. This study evaluated the antioxidant, secondary metabolites, total phenolic content, and total flavonoid content of various crude extracts (methanol, ethanol) and their fractions (50 % and 70 %) of Sargassum polycystum. Fourier transform infrared (FT-IR) analysis was also performed to identify the active chemical composition of the crude extracts. The results showed that various extracts contained varying concentrations of terpenoid, saponin, phlobatannin, cardiac glycosides, phenolic, and flavonoid. FT-IR analysis confirmed the presence of phenols, carboxylic acid, ketones, ethers, aromatics, amides, and sulfonates. 100 % methanolic extract contained the maximum concentrations of total phenols (80.13 mg of GAE/ g) and total flavonoids (38.33 mg of quercetin/g). 100 % methanol extracts exhibited higher antioxidant activity in the DPPH assay (78.32 %, IC50 = 1.59 mg/ml) and ABTS assay (69.82 %, IC50 = 2.39 mg/ml). The biofunctional activity of crude extracts depended on their extract and concentration. The present study demonstrates that the macroalgae S. polycystum contains noteworthy secondary metabolites and natural antioxidants, indicating their potential utilization in various pharmacological and functional food applications.
Phytochemical Screening and Toxicological Evaluation of Sargassum wightii Greville in Wistar Rats
Turkish Journal of Pharmaceutical Sciences, 2019
Bu çalışma, Sargassum wightii'nin farklı ekstrelerinde bulunan fitokimyasalları tanımlamak ve etanol ekstresinin toksisitesini değerlendirmek için yapılmıştır. Gereç ve Yöntemler: S. wightii'den total etanol ekstraksiyonu ve ardışık çözücü ekstraksiyonu yapılarak ekstreler üzerinde ön fitokimyasal tarama yapılmıştır. S. wightii'nin etanol ekstresinin (ESW) akut toksisitesi araştırılmıştır. ESW'nin subkronik toksisitesi, 100, 200 ve 400 mg/kg'lik dozlarla test edilmiştir. Hayvanlar, vücut ağırlığındaki ve yiyecek ve su alımındaki değişiklikler için gözlenmiştir. Çalışmanın sonunda, hayati organların relatif ağırlıkları, ardından histopatolojik incelemeler yapılmıştır. Çeşitli hematolojik ve biyokimyasal değerlendirmeler de yapılmıştır. Bulgular: S. wightii'nin fitokimyasal taraması, alkaloitler, karbonhidratlar, glikozitler, fenolik bileşikler ve tanenlerin varlığını ortaya koymuştur. ESW, akut toksisite çalışmasında mortalite veya terminal öncesi ölüme neden olmamıştır. Hayvanlar, vücut ağırlığındaki ve yem ve su tüketimindeki değişiklikler yönünden gözlenmiştir. Çalışmanın sonunda, hayati organların (beyin hariç) relatif ağırlıkları, yem ve su tüketiminde kontrol grubuna kıyasla anlamlı farklılıklar bulunamamıştır. Histopatolojik incelemede patolojik lezyonların olmadığını gösteren normal bir yapı izlenmiştir. Hematolojik ve biyokimyasal parametreler, yararlı olduğu düşünülen glukoz ve kolesterol seviyelerinde azalma haricinde, kontrol grubu ile de karşılaştırılabilir düzeyde bulunmuştur. Sonuç: S. wightii'de çeşitli fitokimyasalların varlığı, farklı hastalıkların tedavisi için potansiyel bir kaynak olabileceğinin kanıtıdır. ESW ile tedaviden sonra önemli bir toksik etki gözlenmemiştir. Bu nedenle, güvenli olduğu ve uzun süreli tedavide kullanılabilirliği önerilmiştir. Anahtar kelimeler: Sargassum wightii, fitokimyasal tarama, akut toksisite, subkronik toksiste Objectives: The present study was conducted to identify the phytoconstituents present in different extracts of Sargassum wightii and to assess the toxicity of its ethanol extract. Materials and Methods: Successive solvent extraction and total ethanol extraction of S. wightii were performed and preliminary phytochemical screening was carried out. The acute toxicity of ethanol extract of S. wightii (ESW) was studied. The subchronic toxicity of ESW was tested with doses of 100, 200, and 400 mg/kg. The animals were observed for changes in body weight and food and water intake. At the end of the study, the relative weights of vital organs were noted, followed by histopathological examinations. Various hematological and biochemical estimations were also carried out. Results: Phytochemical screening of S. wightii revealed the presence of alkaloids, carbohydrates, glycosides, phenolic compounds, and tannins. ESW did not induce any mortality or pre-terminal death in the acute toxicity study. There were no significant differences in body weight, relative weight of vital organs (except the brain), or food or water intake compared to the control group. The histopathological examination showed normal architecture, suggesting absence of pathological lesions. Hematological and biochemical parameters were also comparable to those of the control group except for reductions in glucose and cholesterol levels, which are postulated to be beneficial. Conclusion: The presence of various phytoconstituents in S. wightii is evidence that it could be a potential source for treating different ailments. No significant toxic effects were observed after treatment with ESW. Thus, it is proposed to be safe and can be recommended for long-term treatment.