Article Tools Print this article Indexing metadata How to cite item Email this article Email the author About The Authors Tri Eko Susilorini Department of Animal Production, Faculty of Animal Husbandry, University of Brawijaya Indonesia Kuswati Kuswati Department of Animal Production, Faculty of ... (original) (raw)
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Kacang goat is a small ruminant native from Indonesia and spread widely in several Southeast Asian countries, and has become part of the world's biodiversity. Growth Hormone (GH) in goat is a protein secreted from anterior hipophyse by somatrotop cells and its formation process under the control of GH gene. Its main function is to stimulate the growth of bone, muscle and fat metabolism. The purpose of this study is to identify the GH gene polymorphism in Kacang goat with Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) methods. A total of 168 blood samples from Kacang goat in two regions of Indonesia (Gorontalo and South Sulawesi province) were extracted by using DNA extraction kit to collect whole DNA genome. Amplification and genotyping of GH gene was performed using PCR-RFLP using the HaeIII restriction enzyme. The results obtained two kinds of genotypes, with the AA genotype frequencies (0.095) and AB (0.904). The frequency of allele A (0.547) and B (0.452) indicate a polymorphism in the GH A781G locus in Kacang goat. The observed (Ho) and expected (He) heterozygosity value were respectively 0.0904 and 0.496. GH alleles distribution in Kacang goat populations were not in Hardy-Weinberg equilibrium. Based on statistical analysis, there is no difference between body size of genotype AA and AB. The results of this study can be used as initial information in formulating a strategy for Kacang goat breeding by utilizing Marker Assisted Selection (MAS).
Polymorphism of the Growth Hormone Gene and Its Effect on Production and Reproduction Traits in Goat
Iranian Journal of Applied Animal Science, 2018
Growth hormone (GH) has an effect on a broad variety of physiological parameters such as lactation, reproduction, growth and metabolism. Thus, the aim of this study was to detect GH gene polymorphism and its association with breeding values of production and reproduction traits in Raini Cashmere goat. Breeding values were estimated using records on 26731 Raini Cashmere goats. To study GH gene polymorphism, 300 animals were selected based on their estimated breeding values (EBVs) for these traits. Then the animal’s genotype was determined using PCR-RFLP. The genotype frequencies for AA, AB and BB were 0.15, 0.85 and 0, respectively. The number of observed alleles, number of effective alleles, expected heterozygosity, observed heterozygosity, mean of heterozygosity, expected homozygosity, observed homozygosity, Nei’s index, Shanon’s index and Fixation index (Fis) were 2, 1.96, 0.49, 0.85, 0.49, 0.51, 0.15, 0.49, 0.69 and -0.74, respectively. Results showed that mean estimated breeding...
Research paper, 2022
In Iraq generally and Kurdistan region especially, goats are an important resource for meat and milk production. It is well known that growth hormone (GH) is involved in a variety of biological activities in livestock animals, including reproduction, growth, lactation, metabolism etc. The goal of this research was to reveal the polymorphism of GH gene in different Capra hircus breeds (native, Shami, Meriz and Kamori goat) and in wild mountain goat (Capra aegagrus) via PCR-RFLP technique and direct seqencing. GH1 (exon 2 and 3) and GH2 (exon 4) polymorphisms on the GH gene were investigated. In all breeds, GH1-Hae III/RFLP revealed only two genotypes (homozygous AA and heterozygous AB), with absent genotype BB. The genotype frequency was 0.886 for the AB genotype and 0.114 for the AA genotype; the allelic frequency was 0.558 for the A allele and 0.442 for the B allele. The average of observed heterozygosity was 0.882 and observed homozygosity was 0.118, indicating that the GH1 was polymorphic. The sequence data of GH1 gene of Meriz and wild goats revealed a SNP at the position 58 (C to T) of the wild goat that has led to a change in amino acid proline to serine. GH2 digestion by Hae III, on the other hand, was monomorphic with the CC homozygous genotype. The results of PCR-RFLP and SNP experiments from this investigation were evaluated as very useful in genotype analysis of local goat breeds/populations. Thus, DNA polymorphisms in the growth hormone gene could be used as reliable genetic markers in breeding programmes in this region.
Genetic polymorphism of five genes associated with growth traits in goat
African Journal of Biotechnology, 2012
Genetic polymorphism studies in domestic animals aim at evaluating genetic variations within and across breeds mainly for conservation purposes. In this study, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect polymorphisms of five candidate genes in four Egyptian and Saudi goat breeds (Barki, Zaribi, Ardi and Masri), to detect the genotype of GH, IGF1, POUIF1, MSTN and BMP15 genes in the goat breeds and their allele frequencies. Results of GH gene which encloses a Haelll endonuclease restriction site show four unique PCR-RFLP banding patterns (genotypes AA, AB, CC and CD). The frequencies of the A allele in the samples from the goat breeds varied from 0.410 to 0.620. While IGF-1gene revealed three fragments after digestion with Haelll with genotype AA, AB and BB and the frequencies of allele A varied from 0.432 to 0.731. Furthermore, PCR-RFLP of POUIF1 gene showed two fragments after digestion by Pst1 endonuclease with genotype TT and CC and the frequencies of allele T varied from 0.250 to 0.840. The MSTN gene revealed three fragments after digestion with DraI with genotype AA, BB and AB and the frequencies of allele A varied from 0.240 to 0.630. Meanwhile, the BMP15 gene revealed one fragments of 112 bp for AA after digestion with Hinf1 enzyme.
International Journal of Chemical Studies, 2020
The present investigation was carried out to study the occurrence of polymorphism in growth hormone (GH) gene in Assam Hill Goat and to associate polymorphic gene, if any, with body weights of the goats at 0 day, 3, 6, 9 and 12 months of age. A total of 256 blood samples were collected from male goats maintained at different field units and at the Goat Research Station, Assam Agricultural University, Burnihat under the “ICAR-All India Coordinated Research Project on Goat Improvement”. The body weights of the same animals were recorded at 0 day, 3, 6, 9 and 12 months of age. PCR-RFLP analysis with Hae III restriction enzyme revealed two genotypes, viz. AA (25.39%) and AB (74.61%) with the gene frequency 0.63 (A) and 0.37 (B). The genotype frequency for AA and AB were found to be 0.40 and 0.60 respectively. Chi-square test revealed that the population was not in Hardy-Weinberg Equilibrium. The mean body weight at 0 day, 3, 6, 9 and 12 months of age for AA and AB genotypes were recorde...
Iranian Journal of Biotechnology, 2009
The growth hormone gene could be an attractive candidate gene for milk production in goats. Single-strand conformation polymorphism was used to identify polymorphism at the goat growth hormone (gGH) gene. For this purpose, genotyping of 90 Talli goat breeds was performed. Nine conformational patterns were observed in exon 4 of the gGH gene, with frequencies of 27.7% for the homozygous pattern (AA) and 72.2% for all of other heterozygous patterns (A/B, A/C, A/B/C, A/B/D/E, A/B/C/F, A/C/F, A/B/E, A/B/F). The results showed that exon 4 of the GH gene in Talli goats is highly polymorphic.
Growth hormone (GH) is a gene that regulates milk production, reproduction, and growth in animals. Therefore, the purpose of this study was to identify GH gene variations and their associations with growth metrics in Sirohi and Assam hill goats. We identified two polymorphic loci (GH1-HaeIII & GH2-HaeIII) which were connected to growth attributes in 161 animals of Assam hill (n = 114) and Sirohi (n = 47) goats using the methods of PCR-RFLP. According to PCR-RFLP results, the GH gene's GH1-HaeIII locus contains two alleles (A and B) and two genotypes (AA and AB), whereas the GH2-HaeIII locus contains two alleles (C and D) and two genotypes (CC and CD). In the two goat breeds, the allele frequencies of A and B were 0.36 − 0.28 and 0.64–0.72, respectively, while the frequencies of alleles C and D were 0.71 − 0.70 and 0.29–0.30. The polymorphic loci were demonstrated to be in a state of Hardy-Weinberg disequilibrium in both breeds of goats (P < 0.05). The growth characteristics o...
Polymorphism of Growth Hormone (GH) Gene in Lakor Goat from Lakor Island of Southwest Maluku Regency
2020
Lakor goat survive in Lakor island in Southwest Maluku with high temperature and limited water. Growth trait in goat is interest to explore cause related with economic trait that encoded by growth hormone (GH) gene. The aim of this study was identify of polymorphism GH gene of Lakor goat in Lakor island. A total of 63 samples were collected from three locations (village) i.e Ketti Letpey (18), Werwawan-Yamluli (26), and Letoda (19). DNA was extracted from hair follicles. A 422 bp specific DNA fragment was successfully amplified and genotyped by PCR-RFLP method using Hae III enzyme. Results showed that polymorphism was found with two variant of genotypes (AA and AB) and two alleles (A and B). AB genotype was dominant in all of populations (93.7%) with A and B alleles were 0.53 and 0.47, respectively. Heterozygosity observed and expected value reached 0.502 and 0.498, respectively while Polymorphic Information Content was in moderate values (0.374). All of populations were in disequil...
SSCP typing of growth hormone gene and its association with birth weight in Black Bengal goat
The Indian journal of animal sciences
Growth hormone either directly or indirectly is the main regulator of postnatal somatic growth, stimulating anabolic process such as skeletal growth and protein synthesis and its deposition in tissues and organs. Therefore, genetic variation of growth hormone gene and its association with birth weight as an indicator of growth performance was investigated in Black Bengal goat. A 245 bp fragment (partial intron 1, exon 2 and partial intron 2) of growth hormone gene was analyzed for detection of polymorphism expected to be present at this locus. SSCP typing revealed 5 genotypes AA, AB, AC, AD and CC and consequently, 4 alleles A, B, C and D were identified. Least square analysis revealed that genotypes had significant effect on birth weight. Animals having AC genotype had highest birth weight whereas animals having CC genotype had lowest birth weight.
Polymorphism of growth hormone gene in three goat breeds in Egypt
Bulletin of the National Research Centre
Background: In Egypt, like other developing countries, goats are prime resources of meat. So, selection of goats for superior growth rate is advantageous. Growth hormone (GH) is the main regulator of animal growth, and encoded by GH gene that exhibits active gene variants improving growth. The objective of this study was to identify GH gene variants in three goat breeds (Barki, Damascus, and Zaraibi), via polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and gene sequencing analyses. Results: Three loci on GH gene named GH1, GH2, and GH6 polymorphisms were analyzed. GH1-HaeIII/RFLP showed only two genotypes (AB and BB) in all breeds, with absent AA genotype. Both Barki and Zaraibi exhibited the highest GC genotype frequency (0.95). GH2-HaeIII/RFLP produced only two homozygous genotypes AA in Damascus and BB in both Barki and Zaraibi, with the absence of AB genotype in the three breeds. However, digestion of GH6 by HaeIII was monomorphic; it exhibited different single-nucleotide polymorphisms (SNPs), detected by DNA sequencing, among the studied goat breeds. Conclusions: The revealed SNPs could be employed as useful markers, helping goat breeders in selection of goats for high growth performance. Further analyses with larger sample size are needed for investigating the relationship between the different genotypes and growth traits.